Tas2r106 (NM_207016) Mouse Tagged ORF Clone

CAT#: MG220117

Tas2r106 (tGFP-tagged) - Mouse taste receptor type 2 member 106 (Tas2r106), (10ug)

Product Images

Circular map for MG220117

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tas2r106
• Synonyms: mGR06; mt2r44; T2R06; Tas2r6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220117 representing NM_207016
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACTGTAGCAGAAGGAATCCTCCTTTGTTTTGTAACTAGTGGTTCAGTCCTGGGAGTTCTAGGAA
  ATGGATTTATCCTGCATGCAAACTACATTAACTGTGTCAGAAAGAAGTTCTCCACAGCTGGCTTTATTCT
  CACAGGCTTGGCTATTTGCAGAATCTTTGTCATATGTATAATAATCTCTGATGGATATTTAAAATTGTTT
  TCTCCACATATGGTTGCCTCTGATGCCCACATTATAGTGATTTCTTACATATGGGTAATTATCAATCATA
  CAAGTATATGGTTTGCCACCAGCCTCAACCTCTTCTATCTCCTGAAGATAGCAAATTTTTCTCACTACAT
  CTTCTTCTGCTTGAAGAGAAGAATCAATACAGTATTTATCTTTCTCCTGGGATGCTTATTTATATCATGG
  TCAATTGCTTTCCCACAAACAGTGAAGATATTTAATGTTAAAAAGCAGCACAGAAATGTTTCCTGGCAGG
  TTTACCTCTATAAGAATGAGTTCATTGTAAGCCACATTCTTCTCAACCTGGGAGTTATATTCTTCTTTAT
  GGTGGCTATCATTACATGCTTCCTATTAATTATTTCACTTTGGAAACATAACAGAAAGATGCAGTTGTAT
  GCCTCAAGATTCAAAAGCCTTAACACAGAAGTACATGTGAAAGTCATGAAAGTTTTAATTTCTTTTATTA
  TCCTGTTAATCTTGCATTTCATAGGGATTTTGATAGAAACATTGAGCTTTTTAAAATATGAAAATAAACT
  GCTACTTATTTTGGGTTTGATAATTTCATGCATGTATCCTTGCTGTCATTCATTTATCCTAATTCTAGCA
  AACAGTCAGCTGAAGCAGGCTTCTTTGAAGGCACTGAAGCAATTAAAATGCCATAAGAAAGACAAGGACG
  TCAGAGTGACATGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220117 representing NM_207016
  Red=Cloning site Green=Tags(s)
  
  MLTVAEGILLCFVTSGSVLGVLGNGFILHANYINCVRKKFSTAGFILTGLAICRIFVICIIISDGYLKLF
  SPHMVASDAHIIVISYIWVIINHTSIWFATSLNLFYLLKIANFSHYIFFCLKRRINTVFIFLLGCLFISW
  SIAFPQTVKIFNVKKQHRNVSWQVYLYKNEFIVSHILLNLGVIFFFMVAIITCFLLIISLWKHNRKMQLY
  ASRFKSLNTEVHVKVMKVLISFIILLILHFIGILIETLSFLKYENKLLLILGLIISCMYPCCHSFILILA
  NSQLKQASLKALKQLKCHKKDKDVRVTW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207016
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207016.1, NP_996899.1
• RefSeq Size: 927 bp
• RefSeq ORF: 927 bp
• Locus ID: 387341
• UniProt ID: Q7M724
• Cytogenetics: 6 63.52 cM
• Gene Summary: Putative taste receptor which may play a role in the perception of bitterness.[UniProtKB/Swiss-Prot Function]