Gramd2 (NM_001033498) Mouse Tagged ORF Clone
CAT#: MG219980
- TrueORF®
Gramd2 (tGFP-tagged) - Mouse GRAM domain containing 2 (Gramd2), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_001033498" in other vectors (6)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Gramd2 |
Synonyms | BC064463; Gramd2a |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219980 representing NM_001033498
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACATTTCCCTCTAGCAACCAACAAATGCACGGGAAGATGGCACCTCTGAAGAGCCATGTGCCCT GCACGGAGAAACCAGGAAAGGTCCAGGAGCCTCCAGATGATGGCAGTCTGCACTGGTCAGAAGGCTCAAA GGGTGAAGACATAAAGAAGTATAGCCGGGAAGGGACACTACGGAGCAAATACAACCAACAATACCACAAG CTGTTTAAAGATATCCCGTTGGAGGAGGTGGTCCTCAAAGTGTGTTCCTGTGCCCTCCAGAGAGACCTCC TTCTCCATGGTCGGCTCTATATCTCTCCCAACTGGCTTTGCTTCCATGCCAGCCTCTTTGGCAAGGATAT CAAGGTGGTCATTCCTGTGGTATCTGTGCAATTGATCAAAAAACACAAGATGGCACGACTCCTTCCCAAT GGACTGGCCATCACCACAAACACCAGCCAGAAGTATGTCTTTGTGTCTCTGCTTTCCCGGGACAGTGTAT ATGACATGCTGAGGAGGGTCTGCACTCACCTGCAGCCTTCCAGCAAGAAGAGTCTGAGTATAAGGAAATT TCCGGAGGAAGCCGAGTGCGAGTCCCCGGAAGTCCTTATCCCCGAGATGAAGTGGAGAAAAGCATGCTCA GCCCCCGCATCCCTGTCACTCCCAGACAGCATCTCTTGTATCTCTCAGATCCCGACAGACTCCACAGATA GCTGCTTCCCCTCCAGGAAGCCTCCAGGGTCTGAGGCCGTCTGTGAGAAAGATGCATTGGAGGAAGAGCC CAGCACGGACCAGGAACTGAGGCTGTGGGATTCTCGGCTCCTCAAGGTCATCTTTGTGATGATCTGCTTT CTAGTCCTGTCCTCGTCCTATCTGGCATTCCGAATCTCCAGGCTGGAACAGCAATTATGTTCCCTGAGCT GGGGCAGTCCACTCCCAAGGGACAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219980 representing NM_001033498
Red=Cloning site Green=Tags(s) MDTFPSSNQQMHGKMAPLKSHVPCTEKPGKVQEPPDDGSLHWSEGSKGEDIKKYSREGTLRSKYNQQYHK LFKDIPLEEVVLKVCSCALQRDLLLHGRLYISPNWLCFHASLFGKDIKVVIPVVSVQLIKKHKMARLLPN GLAITTNTSQKYVFVSLLSRDSVYDMLRRVCTHLQPSSKKSLSIRKFPEEAECESPEVLIPEMKWRKACS APASLSLPDSISCISQIPTDSTDSCFPSRKPPGSEAVCEKDALEEEPSTDQELRLWDSRLLKVIFVMICF LVLSSSYLAFRISRLEQQLCSLSWGSPLPRDR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001033498 |
ORF Size | 936 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033498.2 |
RefSeq Size | 1368 bp |
RefSeq ORF | 939 bp |
Locus ID | 546134 |
UniProt ID | Q3V3G7 |
Cytogenetics | 9 B |
Gene Summary | Participates in the organization ofendoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215137 | Gramd2 (untagged) - Mouse GRAM domain containing 2 (Gramd2), (10ug) |
USD 330.00 |
|
MR219980 | Gramd2 (Myc-DDK-tagged) - Mouse GRAM domain containing 2 (Gramd2) |
USD 300.00 |
|
MR219980L1 | Lenti ORF clone of Gramd2 (Myc-DDK-tagged) - Mouse GRAM domain containing 2 (Gramd2) |
USD 600.00 |
|
MR219980L2 | Lenti ORF clone of Gramd2 (mGFP-tagged) - Mouse GRAM domain containing 2 (Gramd2) |
USD 600.00 |
|
MR219980L3 | Lenti ORF clone of Gramd2 (Myc-DDK-tagged) - Mouse GRAM domain containing 2 (Gramd2) |
USD 600.00 |
|
MR219980L4 | Lenti ORF clone of Gramd2 (mGFP-tagged) - Mouse GRAM domain containing 2 (Gramd2) |
USD 600.00 |
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