Ddit4l (NM_030143) Mouse Tagged ORF Clone

CAT#: MG219940

Ddit4l (tGFP-tagged) - Mouse DNA-damage-inducible transcript 4-like (Ddit4l), (10ug)

Product Images

Circular map for MG219940

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ddit4l
• Synonyms: 1700037B15Rik; 1700108M02Rik; REDD2; RTP801L; Smhs1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219940 representing NM_030143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCAACGGGCAGTTTGAGCAGTAAGAACCCGGCCAGCATTTCAGAGTTGTTGGACGGTGGCTATC
  ACCCTGGGAGTCTGCTAAGTGATTTCGACTACTGGGATTATGTTGTCCCTGAGCCCAACCTCAACGAGGT
  GGTATTTGAAGAGACAACATGCCAGAATTTGGTTAAAATGTTGGAGAACTGTCTGTCCAGATCAAAGCAA
  ACCAAACTTGGTTGCTCTAAGGTCTTGGTCCCTGAGAAACTGACCCAGAGAATTGCTCAAGATGTCCTGC
  GGCTCTCGTCCACGGAGCCCTGCGGCCTTCGGGGTTGTGTTATGCACGTGAACTTGGAGATTGAAAATGT
  ATGTAAAAAGCTGGATAGGATCGTGTGTGATGCTAGCGTGGTGCCGACCTTTGAGCTCACGCTTGTGTTT
  AAGCAGGAGAGCTGCCCGTGGACCAGCCTCAAGGACTTCTTCTTTAGCAGAGGTCGCTTCTCCTCAGGCC
  TTAAGCGAACTCTGATTCTCAGCTCAGGATTTCGACTTGTGAAGAAAAAACTGTACTCCCTGATTGGAAC
  GACAGTCATTGAAGAGTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219940 representing NM_030143
  Red=Cloning site Green=Tags(s)
  
  MVATGSLSSKNPASISELLDGGYHPGSLLSDFDYWDYVVPEPNLNEVVFEETTCQNLVKMLENCLSRSKQ
  TKLGCSKVLVPEKLTQRIAQDVLRLSSTEPCGLRGCVMHVNLEIENVCKKLDRIVCDASVVPTFELTLVF
  KQESCPWTSLKDFFFSRGRFSSGLKRTLILSSGFRLVKKKLYSLIGTTVIEEC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030143
• ORF Size: 579 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030143.4, NP_084419.2
• RefSeq Size: 2721 bp
• RefSeq ORF: 582 bp
• Locus ID: 73284
• UniProt ID: Q8VHZ5
• Cytogenetics: 3 G3
• Gene Summary: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.[UniProtKB/Swiss-Prot Function]