Spinkl (NM_183123) Mouse Tagged ORF Clone

CAT#: MG219298

Spinkl (tGFP-tagged) - Mouse RIKEN cDNA 9530002K18 gene (9530002K18Rik), (10ug)

Product Images

Circular map for MG219298

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spinkl
• Synonyms: 9530002K18Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219298 representing NM_183123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCAACATGGATCAAATTTCTTTTTATTCTGACTTTGGTGCTTCTTCCTTATTCTGTGTTTTCAG
  TGAATATCTTTGCAGGTCCAGAGAATGTTATTAAAGAGCCAAATTGCACCATGTATAAGAGTAAATCTGA
  GTGCTCCAATATCGCTGAGAACCCAGTTTGTGCAGATGATAGAAATACATATTACAATGAGTGTTACTTC
  TGCATAGAAAAAGTAGTGGAAAAACTTAAATATAGGTATCATGGTATTTGTATTTATAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219298 representing NM_183123
  Red=Cloning site Green=Tags(s)
  
  MSSTWIKFLFILTLVLLPYSVFSVNIFAGPENVIKEPNCTMYKSKSECSNIAENPVCADDRNTYYNECYF
  CIEKVVEKLKYRYHGICIYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183123
• ORF Size: 270 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183123.2, NP_898946.1
• RefSeq Size: 593 bp
• RefSeq ORF: 273 bp
• Locus ID: 77424
• UniProt ID: Q8CEK3
• Cytogenetics: 18 B3
• Gene Summary: Does not function as an inhibitor of trypsin, chymotrypsin, subtilisin or elastase. Binds sperm and enhances sperm motility. May act as a decapacitation factor, suppresses BSA-stimulated sperm capacitation and blocks sperm-oocyte interactions in vitro.[UniProtKB/Swiss-Prot Function]