Fads6 (NM_178035) Mouse Tagged ORF Clone

CAT#: MG219099

Fads6 (tGFP-tagged) - Mouse fatty acid desaturase domain family member 6 (Fads6), (10ug)

Product Images

Circular map for MG219099

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fads6
• Synonyms: BC044804; OTTMUSG00000021749
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219099 representing NM_178035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCGTTCGTAGTGCGCCTCCGGGGGACGGCGCTGCTGAGGCGCTGCTGAAAGAGCTGGAGCGTC
  AGGTGCAGGATGTGGTGCGGGCGAGCTCGTGGTGGGAGCGCCACGGTGTGGACTGCGCCATCCTCGCTCT
  CAGCCTGCTCGCCTTGCCGGCAGGGTTCCTGTGCCTACGCGCCCATAATATCCTGGCCTTTGCCACGGGC
  ATCACAATCTTGGGTGTGTGCCATTATACACTCACCGTCAAGGGCAGCCACCTGGCCACTCACAGTGCCC
  TCACAGAGTCCAAACGCTGGAGCAAGATCTTGATGATTTTCTTTTTGGAGGTGTGCACAGCCTTTTCTGC
  AGAGTTCGCCAAATTCAACCACGTGAATCTGCACCACGTTTACACCAACGTGGTGGGTCTGGGCGATTCA
  AGCACGTGGAAGGTGCCTCTCCTCAACCGCTACGTTTACATGTTCCTTGGGCCGCTTTTGGTCCCCATCA
  TAACGCCGCTGGTAGCTCTTGAGCATTTGAGGAAGGAAGAGCCGAGGACTGCACTGCGGACTTTGGGCTT
  CATCTGCCTGGGCCTTTACTCTCAGTACTGGCTGTTCATGAATGTGTCAGGCTTCAAGAACCCCAGTTCA
  GCACTGGCCTGCATGCTGCTCACCAGATCCCTGCTGGCCCACCCTTATCTCCATGTCAATATCTTCCAGC
  ACATCGGACTGCCTATGTTCTCCCCGGACAAGAAGCCCAGGAGGATCCACATGATGACCCTGGGGGTTCT
  GAACCTGCCACGGCAGCTGGTGTTGGACTGGGCATTCGGCCACTCCCTCATCAGCTGCCACGTGGAGCAC
  CACCTCTTCCCTTGGCTCTCTGACCACATGTGCTTGAAGGTGAAGCCCTTGGTGTCGAAGTTTCTCCACG
  AGAAGCAGCTGCCTTACAATGAAGACTCATACCTGGCTCGATTCCAGCTGTTCCTCAGCCGCTATGAGGA
  GTTCATGGTGCATGTCCCGCCCATCACTGAGCTGGTGGGGGTGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219099 representing NM_178035
  Red=Cloning site Green=Tags(s)
  
  METVRSAPPGDGAAEALLKELERQVQDVVRASSWWERHGVDCAILALSLLALPAGFLCLRAHNILAFATG
  ITILGVCHYTLTVKGSHLATHSALTESKRWSKILMIFFLEVCTAFSAEFAKFNHVNLHHVYTNVVGLGDS
  STWKVPLLNRYVYMFLGPLLVPIITPLVALEHLRKEEPRTALRTLGFICLGLYSQYWLFMNVSGFKNPSS
  ALACMLLTRSLLAHPYLHVNIFQHIGLPMFSPDKKPRRIHMMTLGVLNLPRQLVLDWAFGHSLISCHVEH
  HLFPWLSDHMCLKVKPLVSKFLHEKQLPYNEDSYLARFQLFLSRYEEFMVHVPPITELVGVQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178035
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178035.5
• RefSeq Size: 1257 bp
• RefSeq ORF: 1029 bp
• Locus ID: 328035
• UniProt ID: Q80UG1
• Cytogenetics: 11 E2