Wdtc1 (NM_199306) Mouse Tagged ORF Clone

CAT#: MG219000

Wdtc1 (tGFP-tagged) - Mouse WD and tetratricopeptide repeats 1 (Wdtc1), (10ug)

Product Images

Circular map for MG219000

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wdtc1
• Synonyms: adipose; adp; Gm695
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219000 representing NM_199306
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAAGTCAACATAACTAGAGACCTCATTCGTAGGCAAGTCAAGGAGCGGGGTGCCCTGAGCTTTG
  AACGGCGATATCATGTCACAGACCCCTTTATCCGACGGCTGGGCCTGGAGGCGGAGCTTCAGGGACACTC
  AGGCTGTGTCAACTGTCTGGAGTGGAATGAGAAAGGAGACCTGCTGGCGTCTGGTTCCGACGACCAGCAC
  ACCATTGTGTGGGACCCACTGCACCACAAGAAGCTGCTGTCCATGCACACAGGACACACAGCAAATATCT
  TCTCTGTCAAGTTCCTGCCTCATGCTGGAGACCGCATTTTGATCACGGGGGCAGCTGACTCCAAGGTGCA
  CGTCCACGACCTTACGGTAAAGGAGACGATCCACATGTTTGGAGACCACACAAACCGGGTGAAGCGCATT
  GCCACGGCGCCCATGTGGCCTAACACGTTCTGGAGCGCCGCAGAGGATGGGCTTATCCGCCAGTATGACC
  TTCGGGAGAACAGCAAACACTCAGAGGTGCTGATTGACCTAACAGAATACTGTGGCCCGATGGTGGAGGC
  CAAGTGCCTCACCGTCAACCCCCAGGATAACAACTGCCTGGCAGTAGGGGCCAGTGGGCCCTTTGTGAGG
  CTCTATGACATTCGAATGATCCACAACCACAGAAAGAGCATGAGGCAGAGCCCTTCCGCAGGCGTGCATA
  CCTTCTGTGACCGACAGAAGCCCCTTCCGGACGGTGCAGCGCAGTATTATGTCGCAGGTCACCTGCCGGT
  GAAGCTGCCTGACTATAACAGCCGTCTGAGAGTGCTGGTTGCCACCTATGTGACCTTCAGCCCCAATGGC
  ACAGAGCTGCTAGTCAACATGGGAGGAGAGCAGGTGTATCTGTTTGACTTGACTTACAAGCAACGGCCAT
  ATACCTTCCTCTTGCCTAGAAAATGCCACTCCGTGGAAGTCCAGAACGGCAAGATGTCTACTAACGGGGT
  GTCCAACGGCGTGTCGAATGGCCTACACCTTCACAGCAACGGCTTCCGGCTGCCAGAGAGCAAGGGGTGC
  ATCAGCCCCCAGGTGGAGCTGCCCCCATACCTGGAGCGTGTGAAGCAGCAGGCCAATGAAGCCTTCGCCT
  GCCAGCAGTGGACCCAGGCCATCCAGCTCTACAGCCAGGCTGTGCAGAAGGCCCCGCACAACGCTATGCT
  CTATGGCAACCGAGCTGCCGCCTACATGAAGCGCAAGTGGGACGGGGACCACTATGATGCCCTCCGGGAC
  TGCCTCAAGGCCATCTCCCTAAACCCTTGCCACCTGAAGGCACACTTCCGTCTGGCCCGGTGCCTCTTTG
  AACTGAAGTATGTGGCCGAGGCTCTGGAGTGCCTGGATGACTTCAAAGGGAAGTTCCCAGAGCAGGCCCA
  CAGCAGTGCATGTGACGCATTAGGCCGGGACATCACAGCTGCCCTTTTCTCCAAAAGTGATGGTGAGGAG
  AAGAAGGCAGCTGGTGGTGGTGGTGGTCCAGTTCGCCTTCGAAGCACAAGTCGCAAGGACTCCATCTCAG
  AAGACGAAATGGTGCTACGGGAACGGAGCTACGACTATCAGTTCCGCTACTGTGGGCACTGCAACACCAC
  CACGGATATCAAGGAGGCCAATTTCTTTGGCAGCAATGCTCAGTACATTGTCAGCGGCTCTGATGACGGC
  TCCTTCTTCATCTGGGAAAAGGAGACCACCAACCTCGTCCGAGTGCTGCAGGGGGATGAGTCCATTGTCA
  ACTGCTTGCAGCCCCATCCCAGCTACTGCTTCCTGGCCACCAGTGGCATCGACCCTGTTGTGCGACTGTG
  GAACCCACGACCAGAGAGTGAAGACCTCACAGGCCGAGTTGTGGAAGATATGGAGGGTGCATCTCAGGCC
  AACCAGCGGCGCATGAATGCCAACCCCCTGGAGGCGATGCTGCTGGACATGGGCTATCGGATCACAGGCC
  TGAGCAGCGGGGGCGCCGGGGCCTCTGATGACGAGGACAGCGCAGAGGGCCAGGTGCAGTGCCGGCCCAG
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219000 representing NM_199306
  Red=Cloning site Green=Tags(s)
  
  MAKVNITRDLIRRQVKERGALSFERRYHVTDPFIRRLGLEAELQGHSGCVNCLEWNEKGDLLASGSDDQH
  TIVWDPLHHKKLLSMHTGHTANIFSVKFLPHAGDRILITGAADSKVHVHDLTVKETIHMFGDHTNRVKRI
  ATAPMWPNTFWSAAEDGLIRQYDLRENSKHSEVLIDLTEYCGPMVEAKCLTVNPQDNNCLAVGASGPFVR
  LYDIRMIHNHRKSMRQSPSAGVHTFCDRQKPLPDGAAQYYVAGHLPVKLPDYNSRLRVLVATYVTFSPNG
  TELLVNMGGEQVYLFDLTYKQRPYTFLLPRKCHSVEVQNGKMSTNGVSNGVSNGLHLHSNGFRLPESKGC
  ISPQVELPPYLERVKQQANEAFACQQWTQAIQLYSQAVQKAPHNAMLYGNRAAAYMKRKWDGDHYDALRD
  CLKAISLNPCHLKAHFRLARCLFELKYVAEALECLDDFKGKFPEQAHSSACDALGRDITAALFSKSDGEE
  KKAAGGGGGPVRLRSTSRKDSISEDEMVLRERSYDYQFRYCGHCNTTTDIKEANFFGSNAQYIVSGSDDG
  SFFIWEKETTNLVRVLQGDESIVNCLQPHPSYCFLATSGIDPVVRLWNPRPESEDLTGRVVEDMEGASQA
  NQRRMNANPLEAMLLDMGYRITGLSSGGAGASDDEDSAEGQVQCRPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199306
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199306.4, NP_955010.1
• RefSeq Size: 4175 bp
• RefSeq ORF: 2034 bp
• Locus ID: 230796
• UniProt ID: Q80ZK9
• Cytogenetics: 4 D2.3
• Gene Summary: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.[UniProtKB/Swiss-Prot Function]