Papd5 (NM_001164498) Mouse Tagged ORF Clone

CAT#: MG218918

Papd5 (tGFP-tagged) - Mouse PAP associated domain containing 5 (Papd5) transcript variant 2, (10ug)

Product Images

Circular map for MG218918

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Papd5
• Synonyms: 5730445M16Rik; 5830428A09
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218918 representing NM_001164498
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATCTGGGAGACGACCCAGGGGCTGAGGAACCTCTACTTCAACCACCACTGTCACAGCAGCGGCG
  CGAGCAGCGCGAGCGGCGGGAGCGGCAGCGGCCCCGGCAGCCCCGGCGGCACGGCCCCGGCCCCGGCCGG
  CATGTTCCGCTCGGGGGAGCGCCCACTGGGCGGCCTCGCCGTGCCCGCGGAGCAGCGGGACTTCCTGCCC
  CTGGAGACGACCAACAACAACAACAATCACCACCAGCCGGCGGCCTGGGCACGGCGGGCATCGGCGGGCC
  CCTCGGCGTCGCCGGTCCCATCGGCTCCCTCGTCCCCGCGACCGGCGGCCGCACTCCCCGCCTCCGAGTC
  TACCGACCCGGCCTCGGGCAGCAGCAACAAGAGGAAACGTGACAACAAGGCCAGCACCTACGGACTCAAC
  TACAGCCTGCTGCAGCCCAGCGGAGGGCGCGCGGCCGGAGGCGGACGGGCGGACGGCGGCGGGGGCGTGT
  ACAGCGGGACCCCGTGGAAGCGACGGAACTACAACCAGGGAGTCGTGGGTCTGCATGAAGAAATCAGTGA
  TTTTTATGAGTACATGTCTCCCAGACCCGAGGAAGAGAAGATGCGGATGGAGGTAGTGAGCAGGATCGAG
  AGTGTGATTAAAGAGCTCTGGCCCAGTGCTGATGTCCAGATATTTGGAAGTTTTAAAACTGGCCTGTATT
  TACCTACCAGTGACATCGATCTTGTGGTGTTTGGGAAGTGGGAGAACCTGCCTCTCTGGACCCTGGAAGA
  AGCCCTTCGGAAACACAAAGTCGCTGACGAGGATTCCGTGAAAGTTCTAGACAAAGCAACGGTTCCTATT
  ATTAAATTAACAGATTCTTTTACTGAAGTGAAAGTTGATATCAGCTTTAATGTCCAGAATGGCGTGAGAG
  CAGCAGACCTCATTAAAGATTTTACTAAGAAATATCCTGTATTGCCATACTTGGTTTTAGTATTGAAACA
  GTTCTTATTACAGAGGGACCTTAATGAAGTATTTACAGGTGGAATTGGTTCTTATAGTCTCTTTTTAATG
  GCAGTCAGTTTCCTTCAGTTACATCCCAGGGAAGATGCTTGCATCCCCAATACAAACTATGGTGTTCTCT
  TAATAGAGTTTTTTGAATTATATGGACGGCACTTCAATTACTTAAAGACTGGCATCCGGATAAAGGATGG
  TGGTTCCTATGTGGCCAAAGATGAAGTACAGAAAAATATGCTTGATGGCTACCGGCCGTCAATGCTTTAT
  ATCGAAGATCCTTTACAACCAGGTAATGATGTTGGAAGGAGTTCCTATGGGGCCATGCAGGTGAAACAGG
  CCTTTGATTATGCCTATGTGGTATTGAGTCATGCAGTGTCACCGATTGCAAAGTACTATCCCAACAATGA
  AACAGAGAGCATATTAGGTAGAATAATTAGAGTGACGGATGAAGTTGCCACGTATAGAGATTGGATATCA
  AAACAGTGGGGCTTGCAGAATAGGCCCGAGCCATCATGCAACGGAAATGGTGTTACCTTGATAGTAGATA
  CTCAGCAGTTAGATAAGTGTAATAATAATCTGTCTGAAGAAAAGGAAGCCCTTGGAAAATGTAGAAGTAA
  CGCCTCGGAACCTCTTAGTAAACACTCTTCAAACTCTTCATCAGGTCCAGTGTCCTCCTCCTCTGCCACG
  CAGTCCAGCTCTAGTGACGTCGATTCTGATGCAACGCCATGCAAAACCCCCAAACAGCTGCTCTGCCGTC
  CACCCACTGTCACCCGGGTAGGCTCACAGGATGTCTCCTTGGAGGTCTCTCAGGCAGTTGGGAAAATGCA
  GAGCACTCAAACCACTAACACACCCAACAACGCCAACAAATCACAGCATGGATCAGCAAGGCTCTTCCGT
  TCTTCCAGCAAAGGCTTCCAAGGTACAGCTCAAACCAGCCATGGGGCCTTGATGACAAGCAAACAGCATC
  AAGGCAAATCCAATACTCAGTATTACCATGGCAAAAAGAGGAGACACAAGAGGGACGCGCCCCTCTCAGA
  GCTTTGTAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218918 representing NM_001164498
  Red=Cloning site Green=Tags(s)
  
  MQIWETTQGLRNLYFNHHCHSSGASSASGGSGSGPGSPGGTAPAPAGMFRSGERPLGGLAVPAEQRDFLP
  LETTNNNNNHHQPAAWARRASAGPSASPVPSAPSSPRPAAALPASESTDPASGSSNKRKRDNKASTYGLN
  YSLLQPSGGRAAGGGRADGGGGVYSGTPWKRRNYNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVSRIE
  SVIKELWPSADVQIFGSFKTGLYLPTSDIDLVVFGKWENLPLWTLEEALRKHKVADEDSVKVLDKATVPI
  IKLTDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIGSYSLFLM
  AVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAKDEVQKNMLDGYRPSMLY
  IEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYYPNNETESILGRIIRVTDEVATYRDWIS
  KQWGLQNRPEPSCNGNGVTLIVDTQQLDKCNNNLSEEKEALGKCRSNASEPLSKHSSNSSSGPVSSSSAT
  QSSSSDVDSDATPCKTPKQLLCRPPTVTRVGSQDVSLEVSQAVGKMQSTQTTNTPNNANKSQHGSARLFR
  SSSKGFQGTAQTSHGALMTSKQHQGKSNTQYYHGKKRRHKRDAPLSELCR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164498
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164498.1, NP_001157970.1
• RefSeq Size: 4616 bp
• RefSeq ORF: 2043 bp
• Locus ID: 214627
• UniProt ID: Q68ED3
• Cytogenetics: 8 C3
• Gene Summary: Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (By similarity). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (By similarity).[UniProtKB/Swiss-Prot Function]