Klhl7 (NM_026448) Mouse Tagged ORF Clone

CAT#: MG218833

Klhl7 (tGFP-tagged) - Mouse kelch-like 7 (Drosophila) (Klhl7) transcript variant 1, (10ug)

Product Images

Circular map for MG218833

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Klhl7
• Synonyms: 2700038B03Rik; D5Ertd363e; SBBI26
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218833 representing NM_026448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTCCGGCGTGGAAAAGAGTAGCAAGAAGAAAACTGAGAAGAAACTTGCGGCTCGCGAAGAAG
  CCAAGCTGCTGGCGGGGTTCATGGGCGTCATGAACAACATGAGGAAACAGAGAACACTGTGTGACGTGAT
  CCTCACTGTCCAGGAAAGAAAGATCCCCGCTCACCGCGTTGTCCTGGCTGCAGCCAGTCACTTCTTTAAC
  TTAATGTTCACAACTAACATGCTTGAATCTAAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACCTGATA
  TTATTGAACAACTGGTGGAATTTGCTTATACTGCTAGAATTTCTGTGAATAGCAACAATGTTCAGTCTTT
  GTTAGATGCAGCAAATCAGTACCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAA
  GTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTTGCAGAGTGTCTGGACTGTCCTGAACTGAAAGCTA
  CAGCTGATGACTTCATTCATCAGCATTTCACTGAGGTTTACAAAACAGATGAATTCCTGCAGCTTGATGT
  CAAGCGAGTGACACATCTTCTCAGCCAGGACACCCTGACTGTGAGAGCAGAGGACCAGGTTTATGACGCT
  GCCGTGAGGTGGTTGAAATATGATGAGCCTAACCGCCAGCCATTCATGGTTGATATCCTTGCTAAAGTCA
  GGTTTCCTCTTATATCGAAGAATTTCCTAAGTAAAACAGTACAAGCTGAACCACTTATTCAAGATAATCC
  TGAATGCCTTAAAATGGTGATAAGTGGAATGAGGTACCATCTGCTGTCTCCTGAGGATCGAGAAGAACTT
  GCAGGTGGCACACGGCCTAGAAGAAAGAAACATGATTACCGCATAGCTCTGTTTGGAGGCTCCCAACCAC
  AGTCCTGTAGATATTTTAACCCAAAGGATTACAGTTGGACAGACATTCGCTGCCCCTTTGAAAAACGAAG
  AGATGCAGCATGTGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGTTCTCAGCTTTTCCCCATAAAG
  CGAATGGATTGCTACAATGTTGTAAAGGATAGCTGGTATTCCAAACTGGGCCCTCCAACACCTCGAGACA
  GCCTTGCTGCCTGTGCAGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCGGCCCT
  GTATTTGTTTGAATGCTATGATACAAGGACCGAGAGTTGGCACACGAAGCCCAGCATGCTGACTCAGCGC
  TGCAGCCATGGGATGGTGGAGGCCAATGGCCTCATCTATGTTTGTGGTGGAAGCTTAGGAAACAATGTTT
  CTGGGAGAGTGCTTAGTTCCTGTGAAGTGTATGATCCTGCCACAGAAACATGGACTGAGCTGTGTTCAAT
  GATTGAGCCCAGGAAGAATCATGGGCTGGTGTTTGTGAAGGACAAGATATTTGCTGTCGGTGGTCAGAAT
  GGTTTAGGTGGTCTGGACAATGTGGAATATTACGATATCAAGTTAAATGAATGGAAGATGGTGTCACCGA
  TGCCATGGAGGGGTGTCACGGTGAAGTGTGCAGCAGTTGGCTCTGTCATCTATGTCCTGGCAGGTTTTCA
  GGGTGTAGGCAGATTGGGGCATATCCTTGAGTATAATACTGAAACAGATAAGTGGATCGCCAACTCAAAA
  GTCCGGGCTTTCCCAGTCACAAGTTGTCTAATTTGTGTTGTTGATACTTGTGGAGCAAATGAAGAGACCC
  TTGAAACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218833 representing NM_026448
  Red=Cloning site Green=Tags(s)
  
  MATSGVEKSSKKKTEKKLAAREEAKLLAGFMGVMNNMRKQRTLCDVILTVQERKIPAHRVVLAAASHFFN
  LMFTTNMLESKSFEVELKDAEPDIIEQLVEFAYTARISVNSNNVQSLLDAANQYQIEPVKKMCVDFLKEQ
  VDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTDEFLQLDVKRVTHLLSQDTLTVRAEDQVYDA
  AVRWLKYDEPNRQPFMVDILAKVRFPLISKNFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREEL
  AGGTRPRRKKHDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIK
  RMDCYNVVKDSWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQR
  CSHGMVEANGLIYVCGGSLGNNVSGRVLSSCEVYDPATETWTELCSMIEPRKNHGLVFVKDKIFAVGGQN
  GLGGLDNVEYYDIKLNEWKMVSPMPWRGVTVKCAAVGSVIYVLAGFQGVGRLGHILEYNTETDKWIANSK
  VRAFPVTSCLICVVDTCGANEETLET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026448
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026448.3, NP_080724.2
• RefSeq Size: 3427 bp
• RefSeq ORF: 1761 bp
• Locus ID: 52323
• UniProt ID: Q8BUL5
• Cytogenetics: 5 10.67 cM
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination (By similarity).[UniProtKB/Swiss-Prot Function]