Atp7b (NM_007511) Mouse Tagged ORF Clone

CAT#: MG218768

Atp7b (tGFP-tagged) - Mouse ATPase Cu++ transporting beta polypeptide (Atp7b), (10ug)

Product Images

Circular map for MG218768

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp7b
• Synonyms: Atp7a; tx; WND
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218768 representing NM_007511
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCAGGAAGAACTTGGCGTCTGTGGGGACGATGCCTGAACAGGAGAGACAGGTCACAGCCAAAG
  AGGCCAGTCGGAAAATCTTATCTAAACTTGCTTTGCCCGGCCGGCCCTGGGAGCAATCAATGAAGCAGAG
  CTTCGCCTTCGACAATGTTGGCTACGAAGGGGGTCTGGACAGCACCAGCTCGTCCCCAGCAGCCACTGAT
  GTGGTTAACATCCTGGGCATGACTTGTCATTCTTGCGTCAAGTCCATTGAGGACAGGATCTCCAGTCTGA
  AAGGCATTGTGAACATCAAGGTGTCCCTGGAACAAGGCAGCGCCACTGTCAGATATGTGCCGTCTGTCAT
  GAACCTGCAGCAGATTTGCCTTCAGATTGAGGATATGGGCTTTGAGGCCAGCGCTGCAGAGGGAAAGGCC
  GCCTCCTGGCCTTCCAGGTCCTCCCCAGCCCAGGAGGCGGTGGTCAAGCTCCGGGTAGAGGGCATGACCT
  GTCAGTCCTGTGTCAGCTCTATCGAGGGCAAGATCCGGAAACTGCAAGGGGTTGTGAGAATCAAAGTCTC
  CCTAAGCAACCAAGAGGCAGTCATTACCTATCAGCCTTACCTCATTCAACCCGAAGACCTCAGGGACCAC
  ATCTGTGACATGGGATTTGAAGCTGCCATCAAGAACAGAACAGCTCCCTTAAGGCTGGGACCAATTGATG
  TCAACAAGTTAGAAAGCACTAACCTAAAGAAAGAGACGGTCTCTCCTGTCCAGATTTCCAATCATTTTGA
  GACCCTGGGGCACCAGGGGAGCTACCTGGCCACCCTCCCACTAAGAATAGACGGCATGCACTGTAAATCA
  TGTGTTTTGAATATTGAAGGAAATATAGGCCAACTCCCGGGGGTTCAAAATATTCACGTGTCCTTGGAGA
  ACAAAACCGCCCAAATACAGTATGACCCTTCTTGTGTCACCCCCATGTTCCTGCAGACAGCCATCGAGGC
  ACTACCACCTGGGCACTTTAAAGTGTCTCTTCCCGATGGAGTAGAGGAGAATGAGCCCCAAAGTGGCTCC
  TCCCAGAGACACCAGGAGCAGGGCCCGGGCAGGACTGCGGTGCTCACCATCAGCGGCATTACCTGTGCCT
  CATCTGTTCAGCCTATCGAAGACATGCTGTCCCAGAGGAAAGGTGTGCAGCAAACATCCATCTCTTTGGC
  AGAAGGGACTGGAGCAGTTCTTTACGACCCCTCCATAGTCAGCTTGGACGAACTCCGAACGGCTGTAGAA
  GACATGGGATTTGAGGTGTCTGTGAATTCTGAAACCTTCACTATTAACCCTGTCAGAAACTTCAAATCTG
  GGAATTCTGTGCCACAAACCATGGGTGATATAGCAGGGTCTGTGCAAAAAATGGCTCCCGACACTAGAGG
  GCTCCCCACACACCAAGGTCCTGGCCACTCTTCAGAGACCCCATCATCCCCGGGAGCAACAGCATCACAG
  AAGTGCTTCGTACAGATCAAAGGCATGACCTGTGCGTCCTGTGTGTCTAACATAGAAAGGAGTCTCCAGA
  GACATGCTGGTATTCTCTCAGTGTTGGTCGCCTTGATGTCGGGAAAGGCAGAGGTCAAGTATGATCCGGA
  GATCATCCAGTCGCCCAGGATAGCTCAGCTCATCCAGGACCTGGGCTTCGAAGCGTCAGTCATGGAGGAC
  AACACAGTCTCTGAAGGTGACATCGAACTGATTATCACAGGGATGACCTGTGCTTCCTGTGTCCACAACA
  TAGAGTCCAAGCTCACAAGGACAAATGGCATCACTTACGCCTCTGTGGCCCTTGCCACCAGCAAAGCCCA
  TGTGAAGTTCGATCCTGAAATTGTTGGTCCCCGTGACATCATCAAGATCATTGAGGAAATTGGCTTTCAT
  GCTTCCCTGGCCCAGAGAAACCCCAACGCCCATCACTTGGACCACAAGACGGAAATAAAACAGTGGAAGA
  AGTCTTTCCTGTGCAGCCTGGTGTTCGGCATCCCCGTCATGGGATTGATGGTCTACATGTTAATCCCCAG
  CAGTACGCCTCAGGAGACGATGGTCCTGGACCACAACATCATCCCAGGACTGTCCGTTCTCAATCTCATC
  TTCTTCATCTTGTGTACCTTTGTCCAATTTCTGGGTGGGTGGTACTTCTACGTACAAGCCTACAAATCGC
  TGAGACACAGGTCCGCCAACATGGACGTACTCATCGTGCTCGCCACAACCATTGCCTATGCCTACTCCCT
  GGTCATCCTGGTGGTCGCCGTAGCCGAGAAGGCAGAGAAGAGCCCCGTGACCTTCTTTGACACGCCCCCC
  ATGCTCTTTGTGTTCATCGCCCTGGGACGGTGGCTGGAACACGTGGCCAAGAGCAAAACTTCAGAAGCCC
  TTGCAAAACTCATGTCACTCCAAGCCACAGAAGCCACAGTCGTGACCCTGGGTGAGGACAACTTAATCCT
  CAGAGAGGAGCAGGTGCCCATGGAGCTGGTGCAGCGAGGCGACGTCATCAAGGTTGTCCCTGGGGGCAAG
  TTCCCAGTGGATGGGAAAGTCCTCGAAGGCAATACCATGGCTGATGAGTCCCTCATCACAGGAGAGGCCA
  TGCCTGTCACTAAGAAACCTGGGAGCATAGTGATTGCTGGCTCTATTAATGCTCATGGCTCTGTGCTCCT
  TAAAGCTACCCATGTGGGTAATGACACAACTTTGGCTCAGATTGTAAAGTTGGTGGAAGAGGCCCAGATG
  TCAAAGGCTCCCATTCAGCAGCTGGCTGACCGGTTCAGTGGATATTTTGTCCCATTCATCATCATCATTT
  CAACCTTGACCCTGGTGGTGTGGATCGTCATTGGCTTTGTCGATTTCGGTGTGGTTCAGAAGTACTTTCC
  TAGCCCTAGCAAGCACATCTCGCAGACAGAGGTGATCATCCGCTTTGCCTTCCAGACGTCCATCACTGTG
  CTGTGCATCGCCTGCCCCTGCTCCCTGGGGCTGGCCACACCCACAGCAGTCATGGTGGGCACTGGGGTGG
  CTGCCCAGAACGGTGTCCTAATCAAAGGAGGGAAGCCTCTGGAGATGGCACACAAGATAAAGACCGTTAT
  GTTTGACAAAACGGGCACCATCACCCACGGGGTCCCCAGAGTCATGCGGTTCCTGCTGCTCGCAGACGTG
  GCCACACTCCCCCTCAGGAAGGTTCTGGCCGTGGTGGGCACCGCGGAGGCCAGCAGCGAGCACCCCTTAG
  GCGTGGCCGTCACTAAATACTGCAAAGAGGAACTTGGGACGGAGACCCTGGGATACAGCACAGACTTCCA
  GGCAGTGCCCGGCTGTGGAATTAGCTGCAAAGTTAGCAACGTGGAGGGCATCCTGGCTCGCAGTGATCTG
  ACTGCTCACCCTGTTGGAGTTGGCAACCCTCCCACAGGGGAAGGTGCAGGTCCCCAGACCTTCTCCGTGC
  TGATTGGAAACCGGGAATGGATGCGGCGAAACGGTTTAACCATCTCCAGTGACATCAGTGACGCCATGAC
  AGATCACGAGATGAAAGGACAGACGGCCATCCTGGTGGCCATTGATGGTGTGCTCTGCGGGATGATCGCC
  ATCGCAGATGCTGTCAAACCAGAGGCTGCCCTGGCTATCTACACCCTGAAAAGCATGGGTGTGGATGTGG
  CTCTGATCACAGGGGACAACCGGAAGACAGCCAGAGCCATTGCTACTCAGGTTGGCATCAACAAAGTCTT
  TGCGGAGGTACTGCCTTCTCACAAGGTGGCCAAGGTCCAGGAGCTTCAGAATGAAGGGAAGAAAGTCGCC
  ATGGTGGGAGATGGGGTGAATGACTCCCCAGCCCTGGCCCAGGCTGACGTGGGCATCGCCATCGGGACTG
  GCACAGATGTTGCCATCGAAGCAGCAGACGTGGTCCTGATCAGAAATGACTTATTGGACGTCGTGGCCAG
  CATTCATCTCTCCAAGAGGACCGTCCGGAGGATCCGGGTCAATCTGGTGCTGGCATTGATTTATAACATG
  GTTGGGATACCTATTGCTGCAGGTGTCTTCATGCCCATTGGCATCGTGCTGCAGCCGTGGATGGGCTCAG
  CAGCCATGGCTGCGTCCTCTGTCTCTGTGGTGCTCTCGTCTCTTCAGCTCAAGTGCTATAGAAAGCCCGA
  CCTAGAGAGATATGAGGCCCAGGCCCACGGCCGCATGAAGCCCCTGAGTGCCTCCCAAGTCAGCGTGCAC
  ATTGGCATGGATGACCGGCGTCGGGATTCTCCCAGGGCCACCGCGTGGGACCAGGTCAGCTACGTGAGCC
  AAGTGTCTCTGTCCTCCCTGACGTCAGACAGATTGTCTCGGCATGGCGGGGCAGCAGAGGACGGTGGCGA
  CAAATGGTCCCTGCTCCTGAGTGACAGGGATGAAGAGCAGTGCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218768 representing NM_007511
  Red=Cloning site Green=Tags(s)
  
  MDPRKNLASVGTMPEQERQVTAKEASRKILSKLALPGRPWEQSMKQSFAFDNVGYEGGLDSTSSSPAATD
  VVNILGMTCHSCVKSIEDRISSLKGIVNIKVSLEQGSATVRYVPSVMNLQQICLQIEDMGFEASAAEGKA
  ASWPSRSSPAQEAVVKLRVEGMTCQSCVSSIEGKIRKLQGVVRIKVSLSNQEAVITYQPYLIQPEDLRDH
  ICDMGFEAAIKNRTAPLRLGPIDVNKLESTNLKKETVSPVQISNHFETLGHQGSYLATLPLRIDGMHCKS
  CVLNIEGNIGQLPGVQNIHVSLENKTAQIQYDPSCVTPMFLQTAIEALPPGHFKVSLPDGVEENEPQSGS
  SQRHQEQGPGRTAVLTISGITCASSVQPIEDMLSQRKGVQQTSISLAEGTGAVLYDPSIVSLDELRTAVE
  DMGFEVSVNSETFTINPVRNFKSGNSVPQTMGDIAGSVQKMAPDTRGLPTHQGPGHSSETPSSPGATASQ
  KCFVQIKGMTCASCVSNIERSLQRHAGILSVLVALMSGKAEVKYDPEIIQSPRIAQLIQDLGFEASVMED
  NTVSEGDIELIITGMTCASCVHNIESKLTRTNGITYASVALATSKAHVKFDPEIVGPRDIIKIIEEIGFH
  ASLAQRNPNAHHLDHKTEIKQWKKSFLCSLVFGIPVMGLMVYMLIPSSTPQETMVLDHNIIPGLSVLNLI
  FFILCTFVQFLGGWYFYVQAYKSLRHRSANMDVLIVLATTIAYAYSLVILVVAVAEKAEKSPVTFFDTPP
  MLFVFIALGRWLEHVAKSKTSEALAKLMSLQATEATVVTLGEDNLILREEQVPMELVQRGDVIKVVPGGK
  FPVDGKVLEGNTMADESLITGEAMPVTKKPGSIVIAGSINAHGSVLLKATHVGNDTTLAQIVKLVEEAQM
  SKAPIQQLADRFSGYFVPFIIIISTLTLVVWIVIGFVDFGVVQKYFPSPSKHISQTEVIIRFAFQTSITV
  LCIACPCSLGLATPTAVMVGTGVAAQNGVLIKGGKPLEMAHKIKTVMFDKTGTITHGVPRVMRFLLLADV
  ATLPLRKVLAVVGTAEASSEHPLGVAVTKYCKEELGTETLGYSTDFQAVPGCGISCKVSNVEGILARSDL
  TAHPVGVGNPPTGEGAGPQTFSVLIGNREWMRRNGLTISSDISDAMTDHEMKGQTAILVAIDGVLCGMIA
  IADAVKPEAALAIYTLKSMGVDVALITGDNRKTARAIATQVGINKVFAEVLPSHKVAKVQELQNEGKKVA
  MVGDGVNDSPALAQADVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIHLSKRTVRRIRVNLVLALIYNM
  VGIPIAAGVFMPIGIVLQPWMGSAAMAASSVSVVLSSLQLKCYRKPDLERYEAQAHGRMKPLSASQVSVH
  IGMDDRRRDSPRATAWDQVSYVSQVSLSSLTSDRLSRHGGAAEDGGDKWSLLLSDRDEEQCI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007511
• ORF Size: 4386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007511.2, NP_031537.2
• RefSeq Size: 4711 bp
• RefSeq ORF: 4389 bp
• Locus ID: 11979
• UniProt ID: Q64446
• Cytogenetics: 8 10.78 cM
• Gene Summary: Copper ion transmembrane transporter involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.[UniProtKB/Swiss-Prot Function]