Pcnx3 (NM_144868) Mouse Tagged ORF Clone

CAT#: MG218715

Pcnxl3 (tGFP-tagged) - Mouse pecanex-like 3 (Drosophila) (Pcnxl3), (10ug)

Product Images

Circular map for MG218715

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pcnx3
• Synonyms: mKIAA4073; Pcnxl3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218715 representing NM_144868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCTCAGGTGTTGCAGATCCTGCGCCAGGGGGTGTGGGCCTCGCTCACTGGGGGATGGTTCTTCG
  ATCCGCATCAGAGCACCTTCTCCAACTGCTTCCATCTCTATGTCTGGATCTTCTTGCTCATCTTTCCCTT
  CTTGCTGTACATGGTCCTGCCCCCCAGCCTGATGGTGGCTGGAGTGTACTGCCTGGTGGTAGCTGTCATC
  TTTGCTACCATCAAGACTGTGAACTATCGCCTGCATGCCATGTTCGACCAGGGCGAGATTGTGGAGAAAC
  GCAACTCTACCATGGGGGAGCAGGAAGAAGAGGCTGCCCAGGGGGAGAGCAGTCTCCCAAGGGACCCTGG
  TGTGGAGATGACGGTGTTCCGGAAGGTGAGCTCCACGCCCCCTGTACGCTGCAGTTCCCAGCATTCTGTG
  TTTGGCTTCAACCAGGTTTCGGAGTTGCTGCCCCGGATGGAGGATTCTGGGCCCCTCAGAGACATCAAGG
  AGCTGGTACGGGAACAGGGCAGCAACAACGTGATCGTGACCTCTGCCGATCGAGAAATGCTGAAGCTCAG
  CTCCCAAGAGAAACTGATTGGAGACCTTCCCCAGACACCTCCAGGGGTTGTTCCAGACCCCTCTCTCCCC
  AGTACGGATTCTTCCGAGCGTTCTCCCATGGCTGGAGATGGTGTCCCCTGGGGTGGGAGCGGTGTGGCTG
  ACACTCCCATGAGCCCTTTACTGAAAGGGAGCCTCAGCCAGGAGCTAAGCAAGAGCTTTCTGACCCTGAC
  CCGGCCTGACCGGGCCCTAGTGAGGACCAGTAGTCGGCGGGAACAATGTCGGGGAACTGGAGGCTACCAG
  CCCCTGGACCGGAGGGGCTCAGGTGACCCCATGCCCCAGAAAGCTGGCTCTTCGGATTCCTGCTTCAGTG
  GCACTGACAGGGAGACTCTGAGCAGCTTCAAGAGTGAGAAGACTAACTCTACACACCTCGACAGTCCCCC
  TGGTGGGCATGCTCCCGAGGGCAGCGACACAGACCCTCCTTCTGAGGCTGAGCTGCCTGCCTCCCCAGAT
  GCTGGGGTCCCCTCAGATGACACACTTCGTTCCTTTGACACAGTCATTGGAGCAGGGACACCACCGGGCC
  AAACGGAGCCGCTCCTGGTTGTGCGGCCCAAGGACTTGGCCCTGCTTCGGCCTAGCAAGCGGCGGCCCCC
  CATGCGAGGACATTCCCCACCTGGTCGTACCCCAAGACGGCCCTTGCTTGAGGGCTCAGGCTTCTTTGAA
  GATGAAGATACCAGTGAAGGTAGTGAGCTGAGTCCAGCATCCAGTCTCCGGTCTCAGCGCCGCTATAGCA
  CTGATAGCTCCTCGTCTACTTCTTGCTACTCCCCCGAGAGCTCCCAGGGTGCAGCAGGGGGTCCTCGGAA
  GCGGCGGGCCCCTCATGGGGCCGAAGAAGGAACTGCTGTGCCCCCTAAGCGACCCTATGGGACCCAGCGG
  ACGCCCAGTACTGCCAGTGCCAAAACTCATGCCCGTGTGTTGAGCATGGATGGGGCAGGGGGTGATGTCT
  TACGGGCACCCCTGGCGGGCTCCAAGGCTGAGCTGGAGGCCCAGCCAGGAATGGAGCTGGCTGCTGGTGA
  GCCTGCTGTGTTGCCTCCTGAAGCCCGGAGGGGACCCGCTGCCAACCAGCCTGGCTGGCGGGGGGAGCTG
  CAGGAGGAAGGTGCTGTAGGGGGAGCACCTGAGGAAACAGGTCAGCGGGAATGCACAAGCAATGTGAGGA
  GAGCTCAAGCTATCCGGAGACGACACAATGCAGGCAGCAACCCTACGCCTCCAGCCTCTGTCATGGGCTC
  ACCACCTAGCAGCCTGCAGGAGGCTCAGCGGGGCCGGGCTGCTTCCCACTCCAGGGCACTGACTCTGCCC
  TCCGCTCTGCACTTTGCCTCTTCCCTGTTGCTCACCCGAGCTGGCCCCAACGTCCATGAAGCCAGCAATT
  TTGATGACACCTCTGAGGGTGCTGTGCACTATTTCTACGACGAGAGTGGTGTACGACGGTCGTATACCTT
  TGGCCTAGCTGGAGGTGGCTACGAGAACCCTGTGAGTCAGCCAGGCGAGCAGGCAGCCAATGGAGCCTGG
  GACCGTCACTCACATTCCTCCAGCTTCCACTCAGCTGATGTGCCTGAAGCCACGGGAGGCTTGAACCTGT
  TGCAGCCAAGGCCAGTAGTTCTTCAGGGTATGCAGGTGCGCAGGGTGCCCCTGGAAATCCCAGAGGAGCA
  GACACTGATGGAGGAGGCACCACCCCGGGCCCAGCACAGCTACAAGTACTGGTTTCTTCCTGGCCGTTGG
  ACCTCTGTGCGCTACGAACGGCTGGCCCTGCTAGCTCTGTTGGACCGGACACGTGGGGTAATGGAGAACA
  TTTTCGGTGTTGGATTGAGCAGCCTGGTTGCCTTCCTGGGATACCTGTTGCTGCTCAAGGGCTTCTTCAC
  TGACATCTGGGTCTTCCAGTTCTGTCTGGTCATCGCCTCCTGTCAGTACTCCCTGCTCAAGAGCGTGCAG
  CCTGATGCAGCATCCCCAATGCACGGCCACAACTGGGTGATTGCATACAGCCGGCCTGTCTACTTCTGTA
  TCTGCTGTCTACTCATCTGGCTGCTGGATGCCCTGGGGACCGCTCAGCCCTTCCCACCTGTCTCTCTGTA
  CGGCCTCACACTCTTCTCTGCCTCTTTCTTCTTTTGTGCCCGAGATGTGGCCACTGTGTTCACCTTATGC
  TTTCCGTTCGTCTTCCTCCTGGGCCTCCTGCCCCAGGTCAACACCTGCCTCATGTACCTCCTGGAGCAGA
  TAGACATGCATGGCTTTGGAGGCACAGCTGCCACCAGCCCACTCACTGCGGTCTTCAGCCTCACGCGAAG
  CCTGCTGGCTGCTGCCCTGCTTTATGGCTTCTGCCTTGGGGCCATCAAGACACCTTGGCCAGAGCAGCAC
  GTCCCTGTCCTCTTCTCAGTCTTCTGTGGCCTCCTGGTGGCAATGTCCTACCATCTGAGCCGGCAGAGCA
  GCGACCCCACCGTTCTCTGGTCTCTAGTCCGGAGTAAACTCTTCCCTGAGCTAGAGGAGCGGAGCCTAGA
  GACAGCCCGGGTCGAACCCCCAGACCCACTGCCAGAGAAGATGCGTCAGTCAGTGCGGGAAGTCTTGCAC
  TCCGACCTGGTGATGTGTGTGGTGATCGCCGTGCTCACCTTTGCCGTCAGTGCCAGCACCGTCTTCATTG
  CCCTGAAGTCAGTTCTGGGCTTCGTGTTATATGCGCTGGCAGGAGCCGTGGGCTTCTTCACGCATTACCT
  GCTGCCACAGCTGCGCAAACAGCTCCCCTGGTTCTGCCTCTCACAGCCTGTGCTGAAGCCACTGGAGTAT
  AGCCAGTATGAAGTGCGAGGCGCTGCCCAGGTGATGTGGTTTGAGAAGCTCTACGCTGGCCTGCAGTGTG
  CTGAGAAGTACCTCATCTACCCTGCCGTGGTCCTCAACGCTCTCACAGTGGACGCCCACACAGTCGTCAG
  CCACCCAGACAAATTCTGCCTCTACTGCCGGGCCTTGCTGATGACTGTGGCGGGGCTGAAGCTGCTGCGC
  TCGGCCTTCTGTTGCCCACCCCAGCAGTACCTGACCTTGGCCTTCACTGTCCTCCTCTTCCACTTCGACT
  ACCCGAGGCTCTCACAGGGCTTCCTGCTTGACTACTTCCTCATGTCTCTGCTCTGCAGCAAGCTTTGGGA
  CCTGCTGTACAAGCTGCGTTTTGTACTGACCTACATTGCACCATGGCAGATCACCTGGGGCTCAGCCTTC
  CATGCCTTTGCCCAGCCCTTCGCTGTACCACACTCAGCTATGCTGTTCCTTCAGGCCCTGCTCTCAGGGC
  TCTTTTCTACCCCACTCAACCCCCTGCTGGGCAGCGCAGTCTTCATCATGTCCTACGCAAGGCCCCTCAA
  GTTCTGGGAGAGGGACTACAACACTAAACGTGTGGACCATTCTAACACTCGCCTAGTGACACAGCTGGAC
  CGGAACCCAGGCGCTGATGACAACAACCTCAACTCCATCTTCTATGAGCACTTGACACGCTCGCTGCAGC
  ACACGCTGTGTGGGGATCTGGTGCTGGGCCGCTGGGGCAATTATGGCCCTGGAGACTGCTTTGTCCTGGC
  CTCTGACTACCTCAACGCGCTAGTGCACCTCATTGAGGTTGGCAATGGCCTCATCACCTTCCAGCTTCGT
  GGCCTTGAGTTCCGGGGCACATACTGCCAGCAGCGTGAGGTGGAGGCCATCACGGAAGGTGTGGAGGAAG
  ACGAGGGCTGCTGTTGCTGTGAGCCTGGTCACCTGCCCCGGGTCCTGTCCTTCAATGCTGCCTTTGGGCA
  GCGCTGGCTGGCCTGGGAGGTGACAGCCAGCAAGTACGTGCTGGAAGGCTACAGCATCAGTGACAACAAC
  GCAGCCTCCATGCTGCAGGTGTTTGACCTCCGCAAGATCCTCGTCACTTACTATGTCAAGAGCATCATCT
  ACTATGTGAGCCGCTCTCCAAAGCTAGAGACCTGGCTGAACCATGAGGGCATCGCCGCTGCCCTCCGGCC
  TGTGCGAGCCCTTGGTTACGCAGACTCGGACCCCACCTTCTCACTGAGTGTTGACGAGGACTACGACCTC
  CGGTTGTCTGGCCTCTCCCTGCCATCCTTCTGCGCTGTCCACCTCGAGTGGATCCAGTACTGTGCCTCTC
  GGCGCAGCCAGCCCGTGGACCAGGACTGGAATTCACCGTTGGTTACACTGTGCTTTGGCCTGTGTGTGCT
  GGGTCGCCGGGCCCTGGGAACAGCGTCACACAGTATGTCTGCCAGCCTGGAGCCCTTCCTCTATGGCCTG
  CACGCCCTGTTCAAGGGGGACTTCCGCATCACCTCTCCCCGTGACGAGTGGGTCTTTGCCGACATGGATC
  TGCTTCACCGAGTGGTAGCCCCTGGGGTTCGCATGGCCCTCAAGCTTCACCAGGACCATTTCACATCTCC
  TGATGAGTATGAGGAACCAGCAGCCCTGTATGACGCCATCGCGGCCAATGAGGAGCGGCTAGTCATCTCA
  CATGAGGGGGACCCTGCCTGGCGCAGTGCCATCCTCAGCAACACCCCCTCCCTGCTGGCGCTGCGCCATG
  TCATGGACGACGCTTCTGACGAGTACAAGATCATCATGCTCAACAGGCGCCACCTCAGCTTCCGAGTCAT
  CAAGGTAAACCGAGAGTGTGTGCGTGGGCTGTGGGCTGGGCAGCAACAAGAGCTGGTGTTCCTGCGCAAC
  CGCAACCCTGAGCGCGGCAGCATCCAGAATGCCAAGCAGGCACTCCGCAACATGATCAATTCCTCCTGCG
  ACCAGCCACTGGGATATCCCATCTACGTGTCACCTCTTACCACATCTCTGGCTGGTAGCCACCCCCAACT
  GCGGGCGCTGTGGGGTGGTCCTGTCAGCCTGGGTGCCATTGCCCGATGGCTCTTGCGCAGCTGGGAGAGA
  CTTCATAAGGGCTGTGGCGCTGGCTGTAATAGCGGAGGGAATGTGGATGACTCGGACTGTGGTGGGGGTG
  GCGGCCTGACCTCCCTCAGCAATCATCCGCCCTTGGCACACCCTACGCCTGAAAATGCAGCAGGCAGCAG
  TGAGCAGCCCCTCCCACCAGGTCCTAGCTGGGGACCACGGCCTTCCCTCAGTGGCTCTGGTGATGGGCGG
  CCCCCTCCTCTGCTGCAGTGGCCTCCCCCTCGGCTCCCTGGACCACCCCCTGCTTCACCTGCTCCCACTG
  AGGGTCCCCGGCCCTCAAGACCTTCTGGCCCTGCTCTCCTCAATTCTGAGGGACCCAGTGGGAAGTGGAG
  TCTGGGGGGTCGGAAGGGACTGGGAGGACCTGATGGGGAGCCAGCCTCAGGGAGCCCCAAAGGAGGCACC
  CCCAAATCTCAGGCCCCTCTAGACCTCAGCCTCAGTCCTGATGTCAGCTCTGAGGCCTCACCTGCCAGAA
  CCACCCAGGACCTTCCTTGCTTGGACAGCAGTATTCCTGAGGGTTGCACACCCTCCGGTGCCCCAGGTGA
  CTGGCCTGTCCCTGCTGAGGAACGCGAGAGCCCGGCTGCCCAGCCCTTGCTGGAGCATCAGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218715 representing NM_144868
  Red=Cloning site Green=Tags(s)
  
  MGSQVLQILRQGVWASLTGGWFFDPHQSTFSNCFHLYVWIFLLIFPFLLYMVLPPSLMVAGVYCLVVAVI
  FATIKTVNYRLHAMFDQGEIVEKRNSTMGEQEEEAAQGESSLPRDPGVEMTVFRKVSSTPPVRCSSQHSV
  FGFNQVSELLPRMEDSGPLRDIKELVREQGSNNVIVTSADREMLKLSSQEKLIGDLPQTPPGVVPDPSLP
  STDSSERSPMAGDGVPWGGSGVADTPMSPLLKGSLSQELSKSFLTLTRPDRALVRTSSRREQCRGTGGYQ
  PLDRRGSGDPMPQKAGSSDSCFSGTDRETLSSFKSEKTNSTHLDSPPGGHAPEGSDTDPPSEAELPASPD
  AGVPSDDTLRSFDTVIGAGTPPGQTEPLLVVRPKDLALLRPSKRRPPMRGHSPPGRTPRRPLLEGSGFFE
  DEDTSEGSELSPASSLRSQRRYSTDSSSSTSCYSPESSQGAAGGPRKRRAPHGAEEGTAVPPKRPYGTQR
  TPSTASAKTHARVLSMDGAGGDVLRAPLAGSKAELEAQPGMELAAGEPAVLPPEARRGPAANQPGWRGEL
  QEEGAVGGAPEETGQRECTSNVRRAQAIRRRHNAGSNPTPPASVMGSPPSSLQEAQRGRAASHSRALTLP
  SALHFASSLLLTRAGPNVHEASNFDDTSEGAVHYFYDESGVRRSYTFGLAGGGYENPVSQPGEQAANGAW
  DRHSHSSSFHSADVPEATGGLNLLQPRPVVLQGMQVRRVPLEIPEEQTLMEEAPPRAQHSYKYWFLPGRW
  TSVRYERLALLALLDRTRGVMENIFGVGLSSLVAFLGYLLLLKGFFTDIWVFQFCLVIASCQYSLLKSVQ
  PDAASPMHGHNWVIAYSRPVYFCICCLLIWLLDALGTAQPFPPVSLYGLTLFSASFFFCARDVATVFTLC
  FPFVFLLGLLPQVNTCLMYLLEQIDMHGFGGTAATSPLTAVFSLTRSLLAAALLYGFCLGAIKTPWPEQH
  VPVLFSVFCGLLVAMSYHLSRQSSDPTVLWSLVRSKLFPELEERSLETARVEPPDPLPEKMRQSVREVLH
  SDLVMCVVIAVLTFAVSASTVFIALKSVLGFVLYALAGAVGFFTHYLLPQLRKQLPWFCLSQPVLKPLEY
  SQYEVRGAAQVMWFEKLYAGLQCAEKYLIYPAVVLNALTVDAHTVVSHPDKFCLYCRALLMTVAGLKLLR
  SAFCCPPQQYLTLAFTVLLFHFDYPRLSQGFLLDYFLMSLLCSKLWDLLYKLRFVLTYIAPWQITWGSAF
  HAFAQPFAVPHSAMLFLQALLSGLFSTPLNPLLGSAVFIMSYARPLKFWERDYNTKRVDHSNTRLVTQLD
  RNPGADDNNLNSIFYEHLTRSLQHTLCGDLVLGRWGNYGPGDCFVLASDYLNALVHLIEVGNGLITFQLR
  GLEFRGTYCQQREVEAITEGVEEDEGCCCCEPGHLPRVLSFNAAFGQRWLAWEVTASKYVLEGYSISDNN
  AASMLQVFDLRKILVTYYVKSIIYYVSRSPKLETWLNHEGIAAALRPVRALGYADSDPTFSLSVDEDYDL
  RLSGLSLPSFCAVHLEWIQYCASRRSQPVDQDWNSPLVTLCFGLCVLGRRALGTASHSMSASLEPFLYGL
  HALFKGDFRITSPRDEWVFADMDLLHRVVAPGVRMALKLHQDHFTSPDEYEEPAALYDAIAANEERLVIS
  HEGDPAWRSAILSNTPSLLALRHVMDDASDEYKIIMLNRRHLSFRVIKVNRECVRGLWAGQQQELVFLRN
  RNPERGSIQNAKQALRNMINSSCDQPLGYPIYVSPLTTSLAGSHPQLRALWGGPVSLGAIARWLLRSWER
  LHKGCGAGCNSGGNVDDSDCGGGGGLTSLSNHPPLAHPTPENAAGSSEQPLPPGPSWGPRPSLSGSGDGR
  PPPLLQWPPPRLPGPPPASPAPTEGPRPSRPSGPALLNSEGPSGKWSLGGRKGLGGPDGEPASGSPKGGT
  PKSQAPLDLSLSPDVSSEASPARTTQDLPCLDSSIPEGCTPSGAPGDWPVPAEERESPAAQPLLEHQY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144868
• ORF Size: 6084 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144868.3, NP_659117.2
• RefSeq Size: 7281 bp
• RefSeq ORF: 6087 bp
• Locus ID: 104401
• UniProt ID: Q8VI59
• Cytogenetics: 19 A