Gen1 (NM_177331) Mouse Tagged ORF Clone

CAT#: MG218623

Gen1 (tGFP-tagged) - Mouse Gen homolog 1 endonuclease (Drosophila) (Gen1), (10ug)

Product Images

Circular map for MG218623

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gen1
• Synonyms: 5830483C08Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218623 representing NM_177331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGTGAATGACTTATGGCAAATTCTAGAGCCTGTAAAGCAACATATCCACTTGCAAGATCTTAGTG
  GGAAAACCATTGCCGTTGATTTGAGTCTCTGGGTATGCGAGGCACAGACAGTGAAGAAAATGATAGGAAC
  TGTCAAGAAGCCCCACCTCAGGAACCTATTTTTTCGTATTTCATATTTAACTCAAATGAATGTAAAACTG
  GTGTTTGTAATGGAAGGGGAGCCACCAATGCTGAAAGCTGATGTCATAAGCAAGAGGACTCAGACTCGTT
  ACGGGCCTTCTGGAAAATCAAGGTCTCAGAAAACAGGGAGATCACATTTTAAGTCAGTCTTACGAGAGTG
  CCTTGAAATGCTCGAGTGCCTGGGAATGCCCTGGGTCCAGGCCGCCGGGGAAGCTGAGGCCATGTGTGCT
  TACCTTAATGCCAGTGGCCATGTGGATGGCTGCCTCACCAACGACGGCGATGCTTTCCTTTATGGAGCCC
  AGACTGTTTACAGAAATTTCACGATGAACACAAAGGATCCCCATGTTGACTGTTACACGATATCATCTAT
  CAAGAGTAAGCTAGGCTTGGATCGAGACGCTCTGGTTGGACTGGCCGTACTTCTGGGTTGTGATTACCTT
  CCAAAGGGAGTGCCTGGAGTTGGAAAGGAACAAGCATTAAAACTTTTGCAGATTTTCAAAGGTCAAAGTT
  TGCTTCAGAGGTTTAACCAGTGGATTGAAGACCCTTGCTACTCTGTTCCACAATCAGCACCAAAAAAAGT
  GGTTCACTGCTCTGTGTGTTCCCACCCAGGTTCGCCAAAGGATCATGAACGTAATGGATGCATATTATGT
  AAAAGTGATAAATACTGTGAACCACATGATTATGATTACCTCTGCCCTTGTGAATGGCATCAGACAGACC
  ATAATAGGCATCTAAGTGAAATAGAAAACAATATTAAAAAGAAAGCTTGCAGTTGCGAAGGATTTCCATT
  CCATGAGGTCATCCAAGAGTTCCTTTTGAATAAGAATAAAATGTTGAAACCGATCACATACCAAAGACCT
  GATTTATTATTGTTTCAGAGATTTACTGTTCAAAAAATGGAGTGGCCCAGTCACTATGCATGTGAAAAAT
  TGTTGGTGCTTTTGACCCGCTATGACATGATAGAAAGAAAACATGGTAGAAAGACCTCCAATCAACTACA
  GCCAATTAGAATTGTTAAACCCCGAGTCAGAAATGGAGTCCATTGTCTTGAAATAGAATGGGAAAAGCCT
  GAACATTATGTGGTGGAAGATGGAGACCCTGGAAAACTGAGCCTCCTTACAATGGAAGAAGCGTCCCTGT
  TTGAAGCAGCCTATCCTGATGCTGTGGCTGTCTACCAGAAACAGCTGTCAGAGACCAAAGGAAGGAAACA
  GAAAAGTATGAAAAATAAGCCTAAAGGAAGCCATTTACCGGAAGCAGATGATGTGATCAATTCTCAGTCA
  CTTATGACTTTAAAACCTACATCTAAAGCCTTCCCCAAGCAAAATCCCAAAATTAATTTGGAAAATTCTC
  CAGATCCTATTTTAGCACAGGAATCTACTTCCCCCTCATTGAATAGTTTTGTTTCCCCTGAAAATGCTCC
  CTGTTTGAATTTACAAGAACAGTTAGTGCCATCTCCCAGAACTTTGGCTATAAAGCAAAGTAAAGATGTC
  AGTCATTTTCTAGTTTCAGAATGTAGTCAGCCCAGCTCTTCCTCCCATGACATCTCTGTGATTACTGATC
  TGCAGTTGAGTACCATTGATTGGGCGGGTACTTCTTTTAGTAATTCTCCAGCAGTTCAAAGAAACACTTT
  CTCTCAAGACTTAGCATCAGAATCTGAGTCATCGGCCATCCTTCCTGACTTTGAACAGCTGTCATATGAA
  TCAGAACAAGGGACCTCAGACAGTGAAGGGTCCGGTAGAGACCTTCAGCAGAGCAACCCTGAAGAGCAGC
  TCCTTTCTGGCATCAGTGCTTTACATCTTCATGATTTGCCTCTAAAAGAAAGAATACGTATCAAATCTTC
  ATGCCCTCAGTATAATGTAGGAGCAGATGCTGGCCTGGAAAGTTTGCCCCTAAAATTGAAAGGCTCTTGT
  ATTGCTTACAGTAGCTCTGATGGCTCATCCAATTTTTCAAAGGATCTTACAGGGGTATATCTTCACAAAG
  AGTCCAGAAACTCTAAAGTTCTAGACAGCCGACTCCAAGAAAACTGCGGGGCGAATACTTCCTTACCTTA
  TTCTTTCAGTGACAAAGCAGTAAAGACTTCCAGTCTTCAAGTTGGGCTGCCAACTGCTGCTATACCTCAT
  AATCCAAGAGTTGCTGTGAAAACTACTAAGAACCTTGTCATGAAGAATAGTGTTTGCCTCGAGAGAGATT
  CCTCAGATGAAGACAATGCTCCAGGGTCCTGGAAATCAAAGTATACTGCTCCAGAAATGAAACACAGTTC
  TCAGAAGCACAGCCTAGTCCATGTCAGAGACAGTACTCACAACAAACTCAGAAATCCTAAAGTGGAATCT
  AAAGAAACCAAACTGTGTAATGAGTCTTTTAAAACAGCTGAAGATGAAGAAAACGGGTTCTCAGATCTAG
  GGAGAAGTCCTCAGAGTTTTCGACCATGTCATGACAAAGATGAGAACTCTACTGCCTCCTGGGAAAATCC
  TCTACCTTTACGCCAGAGGTTAAAACTCAGGTTCCAGAACACTCAAAGTGGATTTTATAATACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218623 representing NM_177331
  Red=Cloning site Green=Tags(s)
  
  MGVNDLWQILEPVKQHIHLQDLSGKTIAVDLSLWVCEAQTVKKMIGTVKKPHLRNLFFRISYLTQMNVKL
  VFVMEGEPPMLKADVISKRTQTRYGPSGKSRSQKTGRSHFKSVLRECLEMLECLGMPWVQAAGEAEAMCA
  YLNASGHVDGCLTNDGDAFLYGAQTVYRNFTMNTKDPHVDCYTISSIKSKLGLDRDALVGLAVLLGCDYL
  PKGVPGVGKEQALKLLQIFKGQSLLQRFNQWIEDPCYSVPQSAPKKVVHCSVCSHPGSPKDHERNGCILC
  KSDKYCEPHDYDYLCPCEWHQTDHNRHLSEIENNIKKKACSCEGFPFHEVIQEFLLNKNKMLKPITYQRP
  DLLLFQRFTVQKMEWPSHYACEKLLVLLTRYDMIERKHGRKTSNQLQPIRIVKPRVRNGVHCLEIEWEKP
  EHYVVEDGDPGKLSLLTMEEASLFEAAYPDAVAVYQKQLSETKGRKQKSMKNKPKGSHLPEADDVINSQS
  LMTLKPTSKAFPKQNPKINLENSPDPILAQESTSPSLNSFVSPENAPCLNLQEQLVPSPRTLAIKQSKDV
  SHFLVSECSQPSSSSHDISVITDLQLSTIDWAGTSFSNSPAVQRNTFSQDLASESESSAILPDFEQLSYE
  SEQGTSDSEGSGRDLQQSNPEEQLLSGISALHLHDLPLKERIRIKSSCPQYNVGADAGLESLPLKLKGSC
  IAYSSSDGSSNFSKDLTGVYLHKESRNSKVLDSRLQENCGANTSLPYSFSDKAVKTSSLQVGLPTAAIPH
  NPRVAVKTTKNLVMKNSVCLERDSSDEDNAPGSWKSKYTAPEMKHSSQKHSLVHVRDSTHNKLRNPKVES
  KETKLCNESFKTAEDEENGFSDLGRSPQSFRPCHDKDENSTASWENPLPLRQRLKLRFQNTQSGFYNT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177331
• ORF Size: 2724 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177331.5
• RefSeq Size: 3302 bp
• RefSeq ORF: 2727 bp
• Locus ID: 209334
• UniProt ID: Q8BMI4
• Cytogenetics: 12 A1.1
• Gene Summary: Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates.[UniProtKB/Swiss-Prot Function]