Gemin5 (NM_001166671) Mouse Tagged ORF Clone

CAT#: MG218616

Gemin5 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5) transcript variant 4, (10ug)

Product Images

Circular map for MG218616

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gemin5
• Synonyms: AA407055; AA407208; AI451603; BB194447; C330013N08
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218616 representing NM_001166671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCGGAGCCGCGGACGCTGCCGCCGTCCCCCAACTGGTACTGCTCACGCTGCAGCGACGCGGCGC
  CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGTCCGGGTGCGGGCGC
  GAGCCCAGGGGCGCCCCCGTTTCGAGTGGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC
  ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGACGATGGAACTGTGAAGGTTT
  GGGATGTAGAGACCAAGACTGTAGTGACAGAACACACACTCCATCAGCATACAATATCAGCACTGCATTG
  GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTCGTTTTCTGTTACTGGCTTAAC
  AGAAATGACAGCCAGCACCTCTTCACAGAGCCCAGGACCATATTCTGTCTGACGTGTTCACCTCATCACG
  AAAATCTAGTAGCCATTGGCTACAAAGATGGCATAGTGGTTATCATTGACATCAGTAAAAAGGGAGAAGT
  TATTCACAGACTTCGAGGCCACGATGATGAGATCCACTCCATAGCCTGGTGTCCTCTGTCTGGTGAAGAC
  TGCTTATCCATCAGCCAAGAGGAAAACTCGGAAGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAA
  CCCCCATCACAAAAGGCTGCTACTTAGCCACAGGAAGCAAAGATCAGACCATTCGAATCTGGAGCTGTTC
  CCGCGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTCCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACA
  GTTAAAGAACGGCTTTGGTTGACACTTCATTGGCCTAAGAACCAACCAACACAGCTGGTATCCAGTTGTT
  TTGGAGGTGAGCTGCTGCTATGGGACCTCACCCAGTCATGGAGGCGGAAGTACACGCTCTTCAGCACCTC
  AGCAGAAGGGCACAATCATTCCAGAATTGTCTTCAATCTGTGTTCTCTGAAGACTGAAGATGGCAAGCAG
  CTGCTACTGTCCACATCCATGGATAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGA
  CATTGCCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCTCCTGTGGACGTGGGCTCCCTGGCCAT
  AGGTGTTGGGGACGGCATGATCCGGGTGTGGAATACACTCTCCATAAAGAACAACTACGACGTGAAAAAC
  TTCTGGCAAGGAGTCAAGTCCAAGGTCACAGCGCTATGCTGGCACCCAAACAAGGAAGGCTGCTTAGCCT
  TCGGGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCAC
  GTATCATAAGAAGACCGTGTATAGGTTGGCTTGGGGGCCACCAGTCCCCCCCATGTCACTTGGAGGGGAA
  GGAGACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCGTTGTGCTACAGCATAACCCCTGGA
  AGCTCAGTGGAGAGGCCTTCGACATCAACAAACTCGTGAGGGACACCAACTCCATTAGATACAAACTGCC
  CGTGCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCCTGGCTCTTGGGAATGAAGATGGATCAATA
  GAAATATTCCAAGTCCCCAACTTGAGGCTGCTGTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCA
  TAGTCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGTCTCCTTGCTTCTGGCTCCAACAATGC
  AGTCATCTACGTGCACAACCTGAAAGCAGTCCTAGAAAGCAATCCTGACTCTCCAATAACCATCACAGAG
  CCCTACCGCACCCTCTCGGGGCACACAGCCAAGATTACCAGTCTGGCATGGAGCCCACATCATGACGGAA
  GGCTGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGGGAAGAACCTTTGTTCAA
  TTTCCGAGGACATCGAGGCCGGCTGCTATGTGTCGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCA
  GGTGCAGATGACTTCTGTGTCTACAGGTGGCTGACTTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGA
  AAAAATGTATTGAACTAGAGAAAAAACGGCTCTCTCAATTTAAGCCAAAGCTTAAAAAGAAGAAAAAACC
  TACCTTGCGACTGCCTGTAAAGCAGGATTCATCCGTTGGCAATGAAGATGAGAGTGTAAAGGAGAACTCT
  GGACCTGCTGAGAATGGCTTGTCGGACCAGGATGGTGAGGAGGAAGCCCAGGAGCCAGAGCTGCCTCCCT
  CTCCTGTAGTTTGTGTAGAACCTGTTTCCTGTACTGACATTTCCTCAGGCTTTGAAAAGTCAAAAGTCAC
  TGTTAGTAGCAAAGCCACCTCACTGAAAAAGGAGCCACCTAAAGAGAAGCCAGAAGCCTTGCTCAAGAAG
  AGGAAGGCTCGCTCCATGCTCCCTCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAG
  ACTGCTTGGTACTAGCAACTGCAACACACGCCAAAGCAGAACTAAATGAAGATGTGTCTGCCGACCTTGA
  GGAACGATTCCACTTGGGCCTTTTCACAGACAGGGCTACTCTATACAGGATGATGGAAACAGAAGGGAAA
  GGTCACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAAGGAGATCTGAAGGGTG
  TTCTCCAGGCTGCAGCAGAGAGAGGCGAGCTGACAGACAGTCTCGTGGCTGTGGCACCAGTGGCTGGCTA
  CAGTGTATGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGACCAGTACGTCAAGGCT
  GCCTCTTATCTGCTATCCATCCACAAAGTGTACGAAGCTGTGGAACTTCTCAAGTCAAACCATCTCTACA
  GAGAAGCTATTGCAGTTGCCAAGGCTCGCCTGCGCCCTGAGGACCCTGTTCTGAAGGAACTATACCTCAG
  CTGGGGGTCCATCCTGGAGAGAGATGGCCACTATGCTATTGCAGCCAAGTGCTACTTAGGAGCCACTTCC
  GCCTATGATGCAGCCAAAGTTCTGGCCAGAAAGGGAGATGCAGCCTCACTTAGAACAGCTGCAGAGCTGG
  CTGCCATAGCAGGAGAACATGAGCTGGCTGCTTCCCTGGCTCTTAGATGTGCCCAAGAGCTGCTTCTGGT
  GAAGAACTGGGTGGGTGCACAAGAAGCATTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTCTTC
  TGCCTCCTTGAACTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCTAGAGGTCAGAGGCCCCTCTTCCA
  TTTACCACCAGTGGGCCACAGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACCGGTGTGTGGCGCAGCGC
  CTTCAGTGTGGACACCCCGGAGCAGTGTCAGGCCGCCTTACAGAAGCTGCAGGATGTCAAGTACCCATCA
  GCAACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTGACCTTGGCAATGCTGA
  GCCAGCAGGCTGCTGCCTGGGAGGAGGCAGTGCCTGCTCTGCTGCAGGCTGTGGTTCGGAGCTATACCTC
  AGGGAACTTCACCCTCATGCAGGAAATCTACTCAGCCTTTCTCCCAGGTGGCTGTGACCACCTACGAGAC
  AAACTGGGTGACCTCTCTCCTGCCATGGCAGCTTACAAAAGCTTAGAGGCCTTTTGTATTTATGGGCAAC
  TGTATGAAGTCTGGTGGTCGCTCTGTGGGCCTGGCCCTGAATCAAGCGTCTGGGTGTTGTCAGCTGAAAG
  CACGGTCTCTGATAAACAGAGCAAGCCAGAGGACAGTGCCGGTGCTGAGGACATGGAACAGCCTCCAGGC
  CCGGGCCCGAGGCTCAGTGCAGAGAGTGAGCGTTTGCTGAGTGCCTGCAAGGAGCTTTTCTCAGAACGGC
  ATGCCAGCCTGCAGACCTCCCAGAGGACTGTAGCCGAAGTCCAAGAGACACTGGCAGAAATGATCCGCCA
  GCACCAAAAGAGTCAGCTCTGCAAGGCCACAACAAATGGTCCCAGCAGAGATGAACCCAGCAGAGATGAA
  CCCAGCCAGGAAGCAGAGCGGGCTCCTTCTCAGCCTCCGAGCCCAACCAGAGAAGAAAGAAATGCACCAG
  TGTCTCTCCCTGAATTAACCAGAAGGCTCACAGAGGCAAATGAGAGAATAGCAGAGTTTCCAGAGACTGT
  AAAGGCCTGGCCCTTTCCAGATGTGCTGGAGTGCTGTCTCGTCTTGCTTCACATTGGGTCCCAGTGTCCT
  GACGCTGTGGACCCTGAGATGCAGCAACAGGCCCAGGAGCTCCTTCACAAGTATGGGCACACTCGAGCTT
  ACAGAAGACACTGCCAGAGTCGGCACACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218616 representing NM_001166671
  Red=Cloning site Green=Tags(s)
  
  MKPEPRTLPPSPNWYCSRCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGF
  TFSHHPGQYNLCATSSDDGTVKVWDVETKTVVTEHTLHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLN
  RNDSQHLFTEPRTIFCLTCSPHHENLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLSGED
  CLSISQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPT
  VKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCSLKTEDGKQ
  LLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKN
  FWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGE
  GDRPSLTLYSCGGEGVVLQHNPWKLSGEAFDINKLVRDTNSIRYKLPVHTEISWKGDGKVLALGNEDGSI
  EIFQVPNLRLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPDSPITITE
  PYRTLSGHTAKITSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYS
  GADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPVKQDSSVGNEDESVKENS
  GPAENGLSDQDGEEEAQEPELPPSPVVCVEPVSCTDISSGFEKSKVTVSSKATSLKKEPPKEKPEALLKK
  RKARSMLPLSTSLDHRSKEELHRDCLVLATATHAKAELNEDVSADLEERFHLGLFTDRATLYRMMETEGK
  GHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKA
  ASYLLSIHKVYEAVELLKSNHLYREAIAVAKARLRPEDPVLKELYLSWGSILERDGHYAIAAKCYLGATS
  AYDAAKVLARKGDAASLRTAAELAAIAGEHELAASLALRCAQELLLVKNWVGAQEALGLHESLQGQRLVF
  CLLELLCRHLEEKQPLEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCQAALQKLQDVKYPS
  ATSNTPFRQLLLHVCHDLTLAMLSQQAAAWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRD
  KLGDLSPAMAAYKSLEAFCIYGQLYEVWWSLCGPGPESSVWVLSAESTVSDKQSKPEDSAGAEDMEQPPG
  PGPRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATTNGPSRDEPSRDE
  PSQEAERAPSQPPSPTREERNAPVSLPELTRRLTEANERIAEFPETVKAWPFPDVLECCLVLLHIGSQCP
  DAVDPEMQQQAQELLHKYGHTRAYRRHCQSRHT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166671
• ORF Size: 3720 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166671.1, NP_001160143.1
• RefSeq Size: 6105 bp
• RefSeq ORF: 3723 bp
• Locus ID: 216766
• UniProt ID: Q8BX17
• Cytogenetics: 11 B1.3
• Gene Summary: Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3' UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).[UniProtKB/Swiss-Prot Function]