Zc3h12c (NM_001162921) Mouse Tagged ORF Clone

CAT#: MG218239

Zc3h12c (tGFP-tagged) - Mouse zinc finger CCCH type containing 12C (Zc3h12c), (10ug)

Product Images

Circular map for MG218239

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zc3h12c
• Synonyms: A230108E06; C230027N18Rik; mKIAA1726
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218239 representing NM_001162921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGGCCCGGGCGCCGCCGAGGGAAACGGACCCACGGCTGTGATCCTGCCGCTCGGACCCTCGC
  GCGCTCGTGGGGAATACGGGGTGCTTTGCATTCAAGAATACAGGAAAAGCAGCAAAGTGGAGTCAAGTGC
  ACGGAACAGCTTCATGGGCCTGAAGGATCACCTGGGGCATGATCTTGGCCATCTTTATATGGAGAGCACT
  GACCCACAGATGAGTGCAGCTGTGCCTTGGCCTATGGTAGAGAAACCAACAATGGATACAGTCAATTCGG
  GGAAGGAAGGAAAAGGGGTGTCAGAAGAGAATGTGAGCTCCGGCGACTCTGAGGGAAGCACGAGTTCTGA
  TCATGAGTCAGAACAACTGAGTAGCCTTTCGGTAGAGCCATGCTCATTAACCAAGACTCACAGACAGCTA
  TGTCGCTCTCCCTGCTTAGAGCCTCGCCTACTGAAACACAGTGACATTTTGCAAGACTTTAAACCAGAAG
  AGTCCCAGACTCCATCTAAGGAAGTGAAGAAACCCCCTGATGTGGTTCGAGAATACCAAACAAAACTGGA
  GTTTGCACTTAAGTTAGGTTATTCTGAAGAACAGGTCCAGCTTGTGCTAAACAAACTTGGTACTGATGCT
  TTAATCAATGATATATTGGGAGAACTTGTCAAACTTGGAAATAAAAGTGAAGCCGATCAAACAGTTAGTA
  CTATTAACAGCGTAATGCGAGAAACCTCATCCCTGGAGTCTCAGAGGTCTGAATCTCCAATGCAAGAGGT
  TGTAGTAGATGACGGTGAGAATCTGAGACCAGTAGTTATTGATGGCAGCAATGTGGCAATGAGCCATGGA
  AACAAAGAAGTATTCTCGTGCCGAGGAATAAAATTGGCGGTGGATTGGTTTTTGGAAAGAGGCCATAAAG
  ATATCACGGTCTTTGTTCCTGCTTGGAGAAAAGAACAGTCCCGCCCTGACGCCCTCATCACAGATCAGGA
  AATTTTACGTAAACTAGAGAAGGAAAAAATCCTGGTATTCACACCATCACGGCGTGTCCAGGGCAGGCGA
  GTGGTGTGCTATGATGACAGATTCATCGTGAAGCTGGCTTTTGAGTCCGATGGTATAATTGTGTCCAATG
  ATAACTACAGAGATTTGGCAAATGAGAAACCAGAATGGAAGAAGTTCATAGATGAGCGGTTATTGATGTA
  TTCCTTTGTCAATGACAAGTTCATGCCCCCAGATGACCCTCTTGGCAGACATGGCCCAAGTCTGGATAAT
  TTTCTGAGGAAGAAACCCATTGTTCCTGAGCACAAAAAGCAGCCTTGTCCCTATGGAAAGAAGTGTACGT
  ATGGACACAAGTGCAAATACTACCACCCAGAAAGGGGCAGTCAGCCTCAGCGGTCAGTGGCTGATGAACT
  TCGTGCCATGTCTAGAAACACAGCAGCCAAAACTACAAATGAAGGGGGACTGGTGAAAAGCAACAGTGTT
  CCTTGCAGCACCAAGGCAGACAGCACTTCGGATGTTAAACGCGGTGCTCCAAAGAGGCAGTCAGACCCAA
  GCATAAGGACTCACGTCTACCAAGACATAGAGGAGAAGCTTCCCACCAAAAACAAGTTGGAAACCAGGTC
  TGTACCTTCCTTAGTTAGTATCCCGGCCACTTCTACTGCAAAACCCCAAAGCACTACACCTTTAAGCAAT
  GGCCTTCCATCTGGAGTTCATTTCCCACCTCAGGATCAAAGACCACAGGGACAATACCCTCCAATGATGA
  TGGCAACCAAAAATCATGGAACGCCAATGCCTTATGAACAGTATCCAAAATGTGACTCCCCTGTTGACGT
  TGGATATTATTCCATGTTGAATGCATATTCTAATCTGAGTATCTCAGGCCCCCGAAGTCCTGAAAGGCGT
  TTCTCACTAGACACTGATTACAGAGTAAACTCTGTGGCTTCAGACTGCAGCAGTGAAGGGAGCATGAGTT
  GTGGGAGCAGCGACTCCTACGTGGGGTACAATGACCGCTCCTATGTCAGCTCTCCTGACCCACAGCTGGA
  AGAGAGTCTCAAGTGTCAGCACATGCACCCCCACAGCCGCCTTAATTCCCAGCCCTTCCTGCAGAATTTC
  CACGATCCCTTAACCAGAGTCCAGAGTTACAGTCACGAAGAACCAAAGTTCCATCCCAAGCGTCCTCTTC
  CACACTTGGCTATGCATCTGCAGCACCCTGCCGTGGGTGCCCGCTCCAGCTGTCCTGGCGATTACCCCTC
  CCCACCAAGTTCAGCACATTCGAAGGCACCACACCTAGGGAGGTCCTTAGTGGCCACGAGAATAGACAGC
  ATTTCTGATTCCCGACTCTATGATAGTTCTCCTTCAAGACAAAGAAAGCCTTATTCTCGCCAGGAAGGCC
  TAGGAAGCTGGGGTAGGCCAAGCTATGGGCTGGAAGCATATGGCTATCGGCAGACGTACTCCTTACCCGA
  TAATTCCACGCCCCCATGCTATGAGTCGATCACCTTCCAGAGCCTGCCTGAGCAGCAGGAACCCACCTGG
  AGAATCCCCTACTGTGGAATGCCACACGATCCTCCAAGGTACCAGGACAACCGAGAGAAGATTTTTATCA
  ATTTGTGTAACATCTTCCCCCCTGACCTTGTGAGGCTCGTCATGAAAAGGAATCCTCATATGACGGATGC
  CCAGCAGCTCGCCGCAGCCATCTTAGTGGAGAAATCGCAGCTGGGTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218239 representing NM_001162921
  Red=Cloning site Green=Tags(s)
  
  MEAGPGAAEGNGPTAVILPLGPSRARGEYGVLCIQEYRKSSKVESSARNSFMGLKDHLGHDLGHLYMEST
  DPQMSAAVPWPMVEKPTMDTVNSGKEGKGVSEENVSSGDSEGSTSSDHESEQLSSLSVEPCSLTKTHRQL
  CRSPCLEPRLLKHSDILQDFKPEESQTPSKEVKKPPDVVREYQTKLEFALKLGYSEEQVQLVLNKLGTDA
  LINDILGELVKLGNKSEADQTVSTINSVMRETSSLESQRSESPMQEVVVDDGENLRPVVIDGSNVAMSHG
  NKEVFSCRGIKLAVDWFLERGHKDITVFVPAWRKEQSRPDALITDQEILRKLEKEKILVFTPSRRVQGRR
  VVCYDDRFIVKLAFESDGIIVSNDNYRDLANEKPEWKKFIDERLLMYSFVNDKFMPPDDPLGRHGPSLDN
  FLRKKPIVPEHKKQPCPYGKKCTYGHKCKYYHPERGSQPQRSVADELRAMSRNTAAKTTNEGGLVKSNSV
  PCSTKADSTSDVKRGAPKRQSDPSIRTHVYQDIEEKLPTKNKLETRSVPSLVSIPATSTAKPQSTTPLSN
  GLPSGVHFPPQDQRPQGQYPPMMMATKNHGTPMPYEQYPKCDSPVDVGYYSMLNAYSNLSISGPRSPERR
  FSLDTDYRVNSVASDCSSEGSMSCGSSDSYVGYNDRSYVSSPDPQLEESLKCQHMHPHSRLNSQPFLQNF
  HDPLTRVQSYSHEEPKFHPKRPLPHLAMHLQHPAVGARSSCPGDYPSPPSSAHSKAPHLGRSLVATRIDS
  ISDSRLYDSSPSRQRKPYSRQEGLGSWGRPSYGLEAYGYRQTYSLPDNSTPPCYESITFQSLPEQQEPTW
  RIPYCGMPHDPPRYQDNREKIFINLCNIFPPDLVRLVMKRNPHMTDAQQLAAAILVEKSQLGY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162921
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162921.2
• RefSeq Size: 6133 bp
• RefSeq ORF: 2712 bp
• Locus ID: 244871
• UniProt ID: Q5DTV4
• Cytogenetics: 9 A5.3
• Gene Summary: May function as RNase and regulate the levels of target RNA species.[UniProtKB/Swiss-Prot Function]