Adcy9 (NM_009624) Mouse Tagged ORF Clone

CAT#: MG218178

Adcy9 (tGFP-tagged) - Mouse adenylate cyclase 9 (Adcy9), (10ug)

Product Images

Circular map for MG218178

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adcy9
• Synonyms: AC9; ACtp10; AW125421; D16Wsu65e; mKIAA0520
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218178 representing NM_009624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCTCACCCCACCAGCAGCTGCTGCATCACCATAGCACCGAGGTGAGCTGCGACTCAAGCGGAG
  ACAGCAACAGCGTGAGGGTCAAGATCAACCCTAAGCAGCTGTCCTCCAACACCCACCCGAAGCACTGCAA
  GTACAGCATCTCCTCCAGCTGTAGCAGCTCGGGAGACTCAGGGGGCCTTCCCCGGAGGGTTGGCGGCGGG
  GGTCGCCTGCGCAGACAGAAGAAGCTGCCCCAGCTTTTTGAGAGGGCCTCCAGCCGGTGGTGGGACCCCA
  AATTCGACTCCATGAACCTGGAGGAGGCCTGCCTGGAGCGCTGCTTTCCGCAGACCCAGCGCCGCTTCCG
  GTACGCACTCTTTTATGTGGGCTTCGCCTGCCTTCTCTGGAGCATCTATTTCGCTGTCCACATGAAATCC
  AAAGTGATTGTCATGGTGGTCCCAGCTCTGTGCTTCCTGGTGGTGTGTGTGGGCTTTTTCCTGTTTACTT
  TCACCAAGCTGTACGCCCGGCATTATGCGTGGACCTCGCTGGCTCTCACCCTGCTGGTGTTCGCCCTGAC
  CCTGGCTGCGCAGTTTCAGGTTTGGACACCTCTGTCAGGACGTGTTGACAGCTCCAATCATACTCTCACG
  GCCACTCCGGCGGACACTTGCTTATCTCAAGTAGGAAGCTTCTCCATATGCATCGAAGTGCTCCTTTTGC
  TCTACACAGTCATGCAGTTACCTCTGTACCTGAGCTTGTTTTTGGGGGTGGTCTATTCTGTCCTTTTTGA
  GACCTTCGGCTACCACTTCCGAAACGAAGACTGCTACCCTTCTCCGGGCCCTGGGGCCCTGCACTGGGAG
  CTGCTGAGCAGAGCCCTGCTTCACGTGTGCATTCACGCTATCGGGATCCATCTGTTTGTCATGTCTCAGG
  TGAGGTCCAGGAGCACCTTTCTCAAGGTGGGACAATCCATTATGCACGGCAAAGATCTGGAAGTAGAGAA
  AGCCCTGAAAGAGAGGATGATTCATTCAGTGATGCCAAGAATCATAGCCGACGACTTAATGAAACAAGGG
  GACGAGGAGAGTGAGAATTCCGTCAAGAGGCATGCCACCTCCAGTCCCAAGAACAGGAAGAAGAAGTCCT
  CCATACAGAAGGCACCGATAGCATTCCGCCCCTTTAAGATGCAGCAGATTGAAGAAGTCAGTATTTTATT
  TGCAGACATTGTGGGTTTCACCAAGATGAGCGCCAACAAATCTGCGCATGCCTTGGTAGGCCTACTCAAT
  GACCTGTTCGGTCGCTTTGACCGCCTGTGTGAGCAGACCAAGTGTGAGAAGATCAGCACTCTGGGGGACT
  GTTATTACTGTGTGGCAGGGTGTCCGGAGCCCCGGGCAGACCATGCCTACTGCTGCATTGAAATGGGCTT
  AGGCATGATAAAAGCCATCGAGCAGTTCTGCCAGGAGAAGAAAGAGATGGTGAACATGCGTGTTGGGGTT
  CACACGGGGACTGTCCTGTGTGGCATCCTGGGCATGAGGAGGTTTAAATTTGATGTGTGGTCCAACGATG
  TGAACTTGGCTAATCTCATGGAGCAGCTGGGAGTGGCTGGCAAAGTTCACATATCTGAGGCCACTGCAAA
  ATACTTAGACGACAGGTATGAAATGGAAGATGGGAGAGTTATTGAGCGCCTTGGGCAGAGTGTGGTGGCT
  GACCAGTTGAAAGGTTTGAAGACATACCTGATATCGGGTCAGAGAGCCAAGGAGTCCCACTGCAGCTGTG
  CAGAGGCCCTGCTTTCTGGCTTTGAGGTCATTGACGACTCACGGGAGTCCTCAGGCCCTAGGGGACAGGG
  GACAGCATCGCCAGGGAGTGTCAGTGATTTGGCGCAGACTGTCAAAACCTTTGATAACCTTAAGACTTGC
  CCTTCTTGTGGAATCACATTTGCTCCCAAATCTGAAGCTGGTGCAGAAGGAGGAACTGTGCAAAATGGCT
  GTCAAGACGAGCCTAAGACCAGCACCAAGGCTTCTGGAGGACCCAACTCCAAAACCCAGAATGGACTTCT
  GAGCCCTCCTGCAGAGGAGAAGCTCACTAACAGCCAGACCTCCCTCTGTGAGATCCTGCAAGAGAAGGGA
  CGGTGGGCAGGGGTGAGCTTGGACCAGTCAGCCCTCCTCCCGCTCAGGTTCAAGAACATCCGTGAGAAAA
  CTGATGCCCACTTTGTTGATGTCATCAAAGAAGACAGCCTGATGAAAGATTATTTCTTCAAGCCGCCCAT
  CAATCAGTTCAGCCTGAACTTCCTGGACCAGGAGCTGGAGCGATCATATAGAACCAGCTACCAGGAAGAG
  GTCATAAAGAATTCTCCTGTGAAGACGTTCGCCAGTGCCACCTTCAGCTCCCTTCTGGATGTGTTTCTGT
  CAACCACCGTGTTCTTGATTCTCTCCATCACCTGCTTCCTAAAGTATGGAGCCACCGCCACCCCTCCCCC
  ACCGGCTGCCCTGGCCGTCTTTGGTGCAGACCTGCTGCTGGAGGTGCTTTCCCTCATAGTGTCCATCAGA
  ATGGTGTTTTTCCTAGAGGATGTCATGACATGCACAAAGTGGTTGCTGGAATGGATCGCTGGCTGGCTCC
  CTCGCCACTGCATTGGGGCAATCTTGGTGTCTCTTCCTGCCCTGGCTGTCTATTCACACATCACCTCTGA
  GTTTGAGACCAACATACATGTTACCATGTTCACTGGCTCTGCGGTGCTGGTGGCCGTTGTGCACTACTGT
  AACTTCTGCCAGCTCAGCTCCTGGATGAGGTCCTCCCTTGCCACCATCGTGGGGGCTGGGCTGCTGCTTC
  TGCTCCACATCTCCCTGTGTCAGGACAGTTCCATTGTGATGTCCCCCTTGGACTCAGCACAGAATTTCAG
  TGCCCAGAGGAACCCATGCAACAGCTCAGTGCTGCAGGACGGCAGGAGGCCGGCCAGCCTCATAGGCAAG
  GAGCTTATCCTCACCTTCTTCCTCCTGCTCCTCTTGGTCTGGTTCCTGAACCGGGAGTTCGAGGTCAGCT
  ACCGGCTGCACTACCATGGGGATGTGGAGGCCGACCTACACCGCACCAAGATCCAGAGCATGAGAGACCA
  GGCTGACTGGCTACTGCGGAACATCATCCCCTACCATGTGGCTGAGCAGCTCAAGGTCTCTCAGACCTAC
  TCCAAGAACCATGACAGCGGGGGAGTCATCTTTGCCAGCATTGTCAACTTCAGTGAATTCTATGAGGAGA
  ACTATGAGGGGGGCAAGGAGTGCTACCGTGTCCTCAACGAGCTGATCGGTGACTTCGATGAGCTCTTGAG
  CAAGCCGGACTATAATAGCATCGAGAAGATCAAGACCATCGGGGCCACATACATGGCAGCCTCAGGGCTG
  AACACGGCCCAGTGTCAGGAGGGTGGCCACCCACAGGAGCATCTGCGTATCCTCTTCGAGTTCGCCAAGG
  AGATGATGCGCGTGGTGGATGACTTCAACAACAATATGTTATGGTTCAACTTCAAGCTCAGGGTCGGCTT
  TAACCACGGACCCCTCACAGCAGGTGTCATAGGTACCACCAAGCTGCTGTATGACATCTGGGGGGACACC
  GTCAACATCGCCAGCAGGATGGACACCACTGGTGTGGAGTGCCGTATCCAGGTGAGCGAAGAGAGCTACC
  GTGTGCTGAGCAAGATGGGTTATGACTTTGACTACCGAGGGACCGTGAATGTCAAGGGGAAAGGGCAGAT
  GAAGACCTACCTTTACCCAAAGTGCACGGACAATGGAGTGGTTCCCCAGCACCAGCTGTCCATCTCCCCA
  GACATCCGAGTCCAGGTGGACGGCAGCATTGGGCGGTCTCCCACAGATGAGATTGCCAACTTGGTGCCTT
  CCGTTCAGTATTCGGACAAGGCTTCCCTGGGATCTGATGATAGCACACAGGCTAAGGAAGCTCGCCTGTC
  CTCTAAGAGGTCCTGGAGAGAGCCAGTCAAAGCAGAGGAAAGGTTTCCATTTGGCAAAGCCATAGAAAAG
  GACAGCTGTGAAGACATAGGAGTAGAAGAGGCCAGTGAACTCAGCAAGCTCAATGTCTCAAAGAGTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218178 representing NM_009624
  Red=Cloning site Green=Tags(s)
  
  MASSPHQQLLHHHSTEVSCDSSGDSNSVRVKINPKQLSSNTHPKHCKYSISSSCSSSGDSGGLPRRVGGG
  GRLRRQKKLPQLFERASSRWWDPKFDSMNLEEACLERCFPQTQRRFRYALFYVGFACLLWSIYFAVHMKS
  KVIVMVVPALCFLVVCVGFFLFTFTKLYARHYAWTSLALTLLVFALTLAAQFQVWTPLSGRVDSSNHTLT
  ATPADTCLSQVGSFSICIEVLLLLYTVMQLPLYLSLFLGVVYSVLFETFGYHFRNEDCYPSPGPGALHWE
  LLSRALLHVCIHAIGIHLFVMSQVRSRSTFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQG
  DEESENSVKRHATSSPKNRKKKSSIQKAPIAFRPFKMQQIEEVSILFADIVGFTKMSANKSAHALVGLLN
  DLFGRFDRLCEQTKCEKISTLGDCYYCVAGCPEPRADHAYCCIEMGLGMIKAIEQFCQEKKEMVNMRVGV
  HTGTVLCGILGMRRFKFDVWSNDVNLANLMEQLGVAGKVHISEATAKYLDDRYEMEDGRVIERLGQSVVA
  DQLKGLKTYLISGQRAKESHCSCAEALLSGFEVIDDSRESSGPRGQGTASPGSVSDLAQTVKTFDNLKTC
  PSCGITFAPKSEAGAEGGTVQNGCQDEPKTSTKASGGPNSKTQNGLLSPPAEEKLTNSQTSLCEILQEKG
  RWAGVSLDQSALLPLRFKNIREKTDAHFVDVIKEDSLMKDYFFKPPINQFSLNFLDQELERSYRTSYQEE
  VIKNSPVKTFASATFSSLLDVFLSTTVFLILSITCFLKYGATATPPPPAALAVFGADLLLEVLSLIVSIR
  MVFFLEDVMTCTKWLLEWIAGWLPRHCIGAILVSLPALAVYSHITSEFETNIHVTMFTGSAVLVAVVHYC
  NFCQLSSWMRSSLATIVGAGLLLLLHISLCQDSSIVMSPLDSAQNFSAQRNPCNSSVLQDGRRPASLIGK
  ELILTFFLLLLLVWFLNREFEVSYRLHYHGDVEADLHRTKIQSMRDQADWLLRNIIPYHVAEQLKVSQTY
  SKNHDSGGVIFASIVNFSEFYEENYEGGKECYRVLNELIGDFDELLSKPDYNSIEKIKTIGATYMAASGL
  NTAQCQEGGHPQEHLRILFEFAKEMMRVVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDT
  VNIASRMDTTGVECRIQVSEESYRVLSKMGYDFDYRGTVNVKGKGQMKTYLYPKCTDNGVVPQHQLSISP
  DIRVQVDGSIGRSPTDEIANLVPSVQYSDKASLGSDDSTQAKEARLSSKRSWREPVKAEERFPFGKAIEK
  DSCEDIGVEEASELSKLNVSKSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009624
• ORF Size: 4059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009624.3, NP_033754.2
• RefSeq Size: 4457 bp
• RefSeq ORF: 4062 bp
• Locus ID: 11515
• UniProt ID: P51830
• Cytogenetics: 16 2.42 cM
• Gene Summary: Adenylyl cyclase that catalyzes the formation of the signaling molecule cAMP in response to activation of G protein-coupled receptors. Contributes to signaling cascades activated by CRH (corticotropin-releasing factor), corticosteroids and by beta-adrenergic receptors.[UniProtKB/Swiss-Prot Function]