Aldh3a1 (NM_001112725) Mouse Tagged ORF Clone

CAT#: MG217783

Aldh3a1 (tGFP-tagged) - Mouse aldehyde dehydrogenase family 3 subfamily A1 (Aldh3a1) transcript variant 2, (10ug)

Product Images

Circular map for MG217783

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aldh3a1
• Synonyms: Ahd-4; Ahd4; Aldh; Aldh3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217783 representing NM_001112725
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAATATCAGTAGCATCGTGAACCGGGCCCGTGATGCCTTTAACTCAGGCAAGACTCGACCGCTGC
  AGTTCCGGGTTGAGCAGCTGGAGGCGTTGCAGCGCATGATCAACGAGAACTTGAAAGGCATCTCCAAGGC
  GCTGGCCTCTAACCTGCGCAAGAATGAATGGACCTCCTACTACGAGGAGGTGGCTCACGTGCTGGACGAA
  ATCGATTTCACGATTAAGGGGCTCTCCGATTGGGCTGAGGACGAGCCTGTGGCAAAGACTCGTCAGACCC
  AGGAAGATGACCTCTACATCCACTCGGAGCCCCTGGGCGTGGTCCTTGTCATAGGTGCTTGGAACTACCC
  ATTCAACCTCACCATTCAGCCCATGGTGGGTGCCATCGCTGCAGGAAATGCTGTGGTCCTCAAGCCCTCG
  GAAGTGAGTGATCACATGGCAGACCTGCTTTCAACACTTATCCCTCAGTATATGGACAAGGATCTGTACC
  CAGTGATCAAAGGGGGTGTCCCTGAAACCACGGAGCTGCTCAAAGAGAAGTTTGACCACATCATGTACAC
  AGGGAGCACGGCTGTAGGAAAGATCGTCATGGCGGCTGCCGCCAAGCATCTGACCCCTGTCACCTTGGAG
  CTTGGAGGAAAGAGCCCTTGTTACGTAGACAAGGACTGTGATCTAGATGTGGCTTGCCGGCGTATTGCTT
  GGGGAAAGTTTATGAACAGTGGCCAGACCTGTGTGGCCCCAGACTACATCCTCTGTGACCCCTCCATTCA
  GAATGAAATTGTGGAGAAGCTCAAAAAGTCACTGAAAGATTTCTATGGGGAAGATGCTAAGCAGTCCCAT
  GACTATGGGAGGATCATCAACGACCGGCACTTCCAGCGGGTCATAAATCTGATTGACAGCAAGAAAGTAG
  CCCATGGAGGCACCTGGGATCAGCCTTCACGATACATAGCTCCCACCATCCTAGTGGATGTGGACCCCCA
  GTCCCCGGTGATGCAGGAGGAGATCTTTGGGCCGGTGATGCCCATTGTGTGTGTTCGAAGCTTGGATGAG
  GCCATTAAATTTATCAACCAGCGCGAGAAGCCCCTGGCACTCTATGTGTTCTCCAACAATGACAAGGTGA
  TCAAGAAAATGATCGCAGAGACATCAAGCGGTGGAGTGACAGCCAATGACGTCATTGTTCACATCACTGT
  GCCCACTTTGCCCTTTGGGGGTGTGGGGAACAGCGGCATGGGGGCCTACCATGGCAAGAAAAGCTTCGAG
  ACCTTTTCCCACCGCCGCTCTTGCCTGGTGAGGTCTCTGCGGAATGAAGAAGCTAACAAGGCCAGGTATC
  CCCCAAGCCCAGCCAAGATGCCCCGGCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217783 representing NM_001112725
  Red=Cloning site Green=Tags(s)
  
  MSNISSIVNRARDAFNSGKTRPLQFRVEQLEALQRMINENLKGISKALASNLRKNEWTSYYEEVAHVLDE
  IDFTIKGLSDWAEDEPVAKTRQTQEDDLYIHSEPLGVVLVIGAWNYPFNLTIQPMVGAIAAGNAVVLKPS
  EVSDHMADLLSTLIPQYMDKDLYPVIKGGVPETTELLKEKFDHIMYTGSTAVGKIVMAAAAKHLTPVTLE
  LGGKSPCYVDKDCDLDVACRRIAWGKFMNSGQTCVAPDYILCDPSIQNEIVEKLKKSLKDFYGEDAKQSH
  DYGRIINDRHFQRVINLIDSKKVAHGGTWDQPSRYIAPTILVDVDPQSPVMQEEIFGPVMPIVCVRSLDE
  AIKFINQREKPLALYVFSNNDKVIKKMIAETSSGGVTANDVIVHITVPTLPFGGVGNSGMGAYHGKKSFE
  TFSHRRSCLVRSLRNEEANKARYPPSPAKMPRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001112725
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001112725.1, NP_001106196.1
• RefSeq Size: 1597 bp
• RefSeq ORF: 1362 bp
• Locus ID: 11670
• UniProt ID: P47739
• Cytogenetics: 11 37.96 cM
• Gene Summary: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:25286108). Preferentially oxidizes aromatic aldehyde substrates (PubMed:11784860). Comprises about 50 percent of corneal epithelial soluble proteins (PubMed:11784860). May play a role in preventing corneal damage caused by ultraviolet light (PubMed:10376761).[UniProtKB/Swiss-Prot Function]