Arfgef3 (NM_001033258) Mouse Tagged ORF Clone

CAT#: MG217542

D10Bwg1379e (tGFP-tagged) - Mouse DNA segment Chr 10 Brigham & Women's Genetics 1379 expressed (D10Bwg1379e), (10ug)

Product Images

Circular map for MG217542

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arfgef3
• Synonyms: 9630004D11; B930094H20Rik; BIG3; D10Bwg1379e; mKIAA1244
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217542 representing NM_001033258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGATCCTGAGGAAGCTGCAGAGAGATGCGTCCGGGAGCAAATACAAAGCCATCAAAGAGAGCT
  GCACCTGGGCCCTGGAAACCCTAGGTGGCCTGGACACTGTTGTGAAGATCCCTCCCCACTTGTTGAGGGA
  GAAATGCTTGCTGCCTCTCCAGTTGGCTTTGGAATCAAAAAATGTGAAGCTGGCCCAACATGCTTTGGCA
  GGAATGCAGAAGCTCCTGTCAGAAGAGAGATTTGTCTCCATGGAGACAGACTCCGATGAGAAGCAGCTGC
  TCAATCAGATCCTGAATGCAGTGAAAGTGACACCGTCACTCAATGAGGACCTGCAGGTGGAAGTGATGAA
  GGTTTTACTGTGCATCACCTACACCCCGACATTTGACATGAATGGGAGTGCCGTTCTGAAGATCGCAGAG
  GTGTGCATCGAGACCTACACGTGCAGCTGCCACCAGCGCAGTATCAACACTGCCGTGCGTGCAACGCTCA
  GTCAGATGCTGGGTGACCTGACTTTACAGTTACGGCAGAGACAGGAGAATACGATAATTGAAAACCCAGA
  TGCCCCACAGGAATTCAGGAGTCAAGGCTTAACAGTAGAGGCCCTTTGTGATGACGTCATCTCTGTGCTC
  GCAGTTCTGTGTGAGAAGCTCCAAGCCTCCATAAATGACAGCCAGCAGCTACAGCTTCTGTACCTGGAAT
  GCATCCTCTCCGTGCTCAGCAGCTCCTCTTCCTCCATGCACCTGCACAGGGGCTTCACAGACCTCATCTG
  GAAAAGCCTCTGCCCTGCTCTCGTGGTGATCCTGGGGAACCCTATTCACGACAAAACAATCACCTCTGCT
  CACAGCACCAGCACCAGCACCAGCATGGAGTCGGACTCTGCCTCTCTTGGTGTGTCTGACCACGGCCGGG
  GCTCAGGCTGCTCCTGCACTGCACCCACCCTCAGTGGGCCTGTGGCCCGCACCATCTATTACCTTGCAGC
  TGAGCTTGTCCGCTTAGTGGGGTCAGTGGACTCCATGAAGCCAGTGCTCCAGTCCCTCTATCACCGAGTG
  CTCCTGTATCCCCCACCCCAGCACCGAGTGGAAGCCATCAAGATCATGAAAGAGATACTTGGGAGCCCAC
  AGCGTCTGTATGACTTGGCAGGACCAAGCTCTATTGAGTCTGAACCAAGGAAAAGATCTATTTCCAAAAG
  AAAGTCTCACTTGGATCTTCTCAAACTCATCATGGACGGCATGACAGAAGCATGCATCAAAGGAGGCATT
  GAAGCCTGCTACGCTGCTGTGTCCTGTGTCTGCACCTTGCTTGGGGCTCTGGATGAACTCAGCCAAGGGA
  AAGGCTTGAATGACACTCAAGTGCAGCAGCTGCTTCTGCGCCTTGAAGAGCTGAGGGATGGGGCCGAGTC
  GAGCCGGGACTCCATGGAAATTAATGAGGCTGACTTCCGCTGGCAGCGGAGAGTGCTGTCTTCGGAGCAC
  ACTCCATGGGAGTCAGGCAATGAGAGGAGCCCTGACATCAGCATCAGTGTCACCACAGACACAGGCCAGA
  CTACCTTGGAAGGAGAATTGGGTCAGACGACACCAGAGGACCACAAGAATGGGCTCAAGTCACCAGCCAT
  TCAGGAAGGCAAGGGGACCATGGGCAAAGTGTCAGAACCTGAGGCTATAGACCAGCCAGATGTGGTTCAG
  AGAAGCCACACAGTCCCTTACCCCGATATCACCAACTTCCTGTCAGTGGACTGCAGGACAAGGTCCTACG
  GATCTAGGTATAGTGAGAGCAACTTCAGTGTAGATGACCAAGACCTGTCGAGGACTGAATTTGATTCCTG
  TGATCAGTATTCCATGGCGGCAGAAAAGGACTCAGGGAGGTCTGATGTGTCAGACATTGGGTCAGACAAC
  TGTTCCCTGGCTGATGAAGAACAGACCCCTCGGGACTACATAGGCCATCGGTCCCTTCGAACCGCTGCTT
  TGTCTCTCAAACTGCTAAAGAACCAGGAGGCAGACCAGCACAGCGCCCGACTGTTTATACAGTCCCTTGA
  GGGCCTCCTACCCCGGCTGCTGGCCCTGTCCAGTGTGGAAGAGGTGGACTCCGCCCTTCAGAACTTTGCC
  TCTACTTTCTGCTCAGGCATGATGCACTCTCCTGGCTTTGATGGAGGCAGTAGCCTGAGCTTCCAGATGC
  TGATGAATGCAGACAGCTTGTACACAGCGGCACACTGTGCCCTACTGCTCAACCTGAAGCTCTCCCACGG
  TGACTACTACCGGAAGCGACCAACCGTGGCACCAGGCATGATGAAAGAGTTCATGAAACAGGTGCAGACC
  AGCGGCGTGCTCATGGTCTTCTCCCAGGCCTGGCTCGAGGAACTGTACCATCAGGTGCTGGACAGAAACA
  TGCTGGGAGAAGCTGGCTACTGGGGCAGCCCTGAGGACAACAGCCTTCCCCTCATCACCATGCTCACCGA
  TATCGATGGTCTAGAGAGTAGCGCAATCGGTGGCCAGTTGATGGCGTCAGCTTCAGTGGAGTCACCTTTC
  ACCCAGAGCAGGAGACTTGATGACTCCACGGTAGCAGGTGTTGCATTTGCCCGTTATATTTTGGTTGGCT
  GCTGGAAGAACCTGATTGATACTCTGTCAACCCCCTTGACTGGTCGGATGGCAGGAAGCTCCAAGGGGTT
  GGCCTTCATCCTTGGAGCTGAGGGTATCAAAGAGCAGAACCAGAAAGAGCGAGATGCCATCTGCATGAGC
  CTTGATGGGCTAAGGAAAGCTGCGCGACTGAGCTGTGCACTCGGGGTCGCTGCTAACTGTGCCTCAGCTC
  TTGCCCAAATGGCAGCTGCATCCTGTGTCCAAGAAGAGAAAGAAGAAAGGCAATCCCAAGAGCCCAGTGA
  TGCCCTAGCACAAGTGAAACTAAAAGTGGAGCAGAAACTGGAGCAGATGGGGAAGGTGCAGGGGGTGTGG
  CTGCACACAGCCCACGTCTTGTGCATGGATGCCATTCTCAGCGTAGGCCTGGAGATGGGAAGCCACAACC
  CGGACTGCTGGCCACACGTGTTCAGGGTGTGTGAGTATGTGGGTACACTGGAGCACACCCACTTCAGCGA
  TGGTATCTCCCAGCCCCCTCTGACCATCCATCAGCCACAGAAGACATCTGGGAGCTCTGGCCTCCTTGGG
  GAAATTGAGTTTAAGAGCTCATCCCAGGAGCAGAGCCTGGAGCAGGGGCCTTCCCTGAACACAGCCCCTG
  TGGTCCAGCCACACTCCATCCAGGAGCTTGTTCGGGAATGTAGCCGTGGCCGGACCTCGGACTTCCGGGG
  AGGTAGTCTGAGTGGGAACAGTGCTGCCAAGGTGGTGCTCAGCCTTTCCACCCAGGCCGACAGGCTCTTT
  GATGACGCGACCGACAAGTTGAACTTAACAGCCTTGGGAGGGTTCCTCTACCAACTCAAAAAAGCATCTC
  AGTCCCAGCTTTTCCATTCGGTGACAGACACAGTCGATTACTCTCTGACAATGCCAGGTGAAGTCAAGTC
  TACCCAGGATCAAAAAAGCGCTCTCCACCTGTTCCGCCTGGGGGATGCCATGCTGAGGATTGTGAGGAGC
  AAAGCCCGGCCCCTGCTCCACGTGATGCGCTGCTGGAGCCTCGTGGCCCCGCACCTGGTGGAGGCTGCCT
  GCCATAAAGAACGCCATGTGTCTCAGAAGGCCGTTTCCTTCATCCATGATATCTTGACAGAGGTTCTCAC
  TGACTGGAGTGAGCCACCTCACTTCCACTTTAACGAAGCACTCTTCCGGCCCTTTGAGCGCATCATGCAG
  CTAGAGCTCTGTGATGAAGATGTCCAAGACCAGGTGGTCACCTCCATTGGTGAGCTGGTTGAGGTGTGCT
  CTGCACAGATCCAGTCGGGATGGCGACCCCTGTTCAGTGCTCTGGAGACCGTGCGCAGTGGGAACAAGTC
  AGAGGTGAAGGAGTACCTGGTTGGGGACTACTCTATGGGAAAAGGCCAGGCTCCTGTGTTCGATGTGTTT
  GAAGCTTTTCTCAATACCGACAACATCCAGGTCTTTGCTAACGCAGCTACTAGTTACATCATGTGCCTTA
  TGAAATTTGTCAAGGGGCTGGGGGAGGTAGACTGTAAAGAGATTGGAGACTGTGTTCCTGGAGCTGGAGC
  TACATCTACAGACCTGTGCCTGCCTGCCTTGGATTACCTCAGACGTTGTTCTCAGTTATTGGCCAAGATC
  TACAAAATGCCCTTAAAGCCAATATTCCTTAGTGGGCGACTTGCCAGTTTGCCACGAAGGCTTCAGGAGC
  AGTCGGCAAGCAGTGAAGATGGAATTGAATCAGTCCTGTCAGATTTTGATGATGACACTGGTCTGATAGA
  AGTCTGGATCATCCTGTTGGAGCAGCTAACAGCAGCTGTGTCCAACTGTCCAAGGCAACACCAACCACCC
  ACCTTAGATTTACTCTTTGAGCTGTTGAGAGATGTTACCAAAACACCTGGCCCTGGGTTTGGAATCTATG
  CAGTTGTTCACCTTCTCCTTCCTGTGATGTCTCTGTGGCTCCTCCGTAGCCACAAGGACCATTCCTACTG
  GGATGTGGCATCTGCTAACTTCAAGCACGCCATTGGTCTGTCCTGCGAGCTGGTGGTGGAACACATTCAA
  AGCTTTCTACACTCAGACATCAGGTATGAGAGTATGATCAACACCATGCTGAAGGATCTCTTTGAGCTAC
  TGGTAGTATGCGTGGCCAAGCCCACAGAAACCATCTCCAGGGTGGGCTGTTCCTGCATTAGGTACGTCCT
  CGTGACAGCAGGTCCCGTGTTCACGGAGGAGATGTGGAGACTTGCATGCTGTGCCCTTCAGGACGCCTTC
  TCAGCCACCCTCAAGCCAGTGAAGGACCTGCTAGGCTGCTTCCACGGTGGTACAGAGGGCTTCAGTGGGG
  AAGGCTGCCAGGTGCGCGTAGCAGCACCCTCTTCCTCCCCGAGTGCTGAGGCTGAGTACTGGCGCATCCG
  GGCCATGGCCCAGCAGGTGTTTATGTTGGACACCCAGTGCTCACCAAAGACTCCAAACAACTTCGATCAC
  GCTCAGTCCTGTCAGCTCATCATTGAACTGCCTCATGATGAGAAACCAAATGGACATGCCAAGAAAAGTG
  TCTCTTTCAGGGAGATTGTGGTTAGCTTGCTCTCGCACCAAGTTCTGCTCCAGAACCTGTATGACATTCT
  GCTGGAAGAGTTTGTCAAAGGCCCCTCTCCGGGAGAAGAGAAGACAGTCCAAGTACCAGACACCAAGCTG
  GCCGGCTTCCTCAGATACATTTCCATGCAGAACCTGGCTGTGATATTTGACCTGCTGCTGGACTCCTATA
  GGACTGCCCGGGAATTCGACACCAGCCCAGGGCTGAAATGCCTGCTGAAGAAAGTGTCTGGCATTGGAGG
  AGCAGCCAACCTCTACCGCCAGTCGGCCATGAGCTTTAACATTTACTTTCACGCCCTGGTTTGCGCGGTT
  CTCACCAACCAAGAAACTATCACAGCCGAGCAAGTAAAGAAGGTGCTATTCGAAGAGGAGGAGAGGAGCT
  CGGATTCCTCCCAACAGTGCTCGTCGGAGGATGAAGACATCTTTGAGGAGACTGCGCAGGTGAGCCCCCC
  ACGAGGCAAGGAGAAAAGGCAATGGCGGGCACGCCTGCCGTCGCTCAGTGTGCAGCCAGTAAGCAATGCA
  GACTGGGTGTGGCTGGTCAAGAGGCTGCACAAGCTGTGCATGGAGCTCTGTAACCACTACATCCAGATGC
  ACCTGGACCTGGAGAGCAGCTTGGAGGAGCCACTTACCTTCAAGAGCGACCCGTTCTTCATTCTCCCCTC
  CTTCCAGTCCGAATCGTCCACCCCATCAACAGGTGGCTTCTCCGGGAAAAACACCCCCTCGGAAGATGAC
  CGCAGGGAGCACCTGAGTGAGCCTCAGAGTCTGAGGGTCGGCAGTGGGGACATGCTGATGCTGCCTCCCA
  GCCCCAAGACAGAGAAGAAGGATCCTGGCCGCAAGAAAGAGTGGTGGGAGAGTGCAGGGAACAAGATTTG
  CACAATGGCCGCTGACAAGACCATCTCAAAGCTGATGACTGAGTACAAGAAGAGAAGGCAGCCACATAAC
  CTGCCCCCCTTCCCCAAGGAAGTCAAAGTAGATAAGAAAGGGGAGCCCTTGGGGCCCCGGGGTCCTGACT
  CCCCACTGCTTCAGCGGCCACAGCATCTCATAGACCAAGGGCAGATGCGCCATTCCTTCAGCGCAGGCCC
  AGAGCTGCTGAGGCAGGAGAAGAGACCTCGGTCAGGCTCCACAGGAAGCTCCCTTAGTGTCTCTGTACGG
  GATGCAGAAGCGCAGATCCAGGCGTGGACCAACATGGTGCTAACTGTTCTCAATCAGATTCAGATCCTTC
  CAGACCAGACCTTCACTGCTCTCCAGCCAGCTGTGTTCCCATGCATCAGCCAGCTGACCTGTCACGTGAC
  TGACATCCGAGTCCGTCAAGCTGTGAGGGAATGGCTGGGAAGAGTGGGCCGTGTCTATGACATCATCACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217542 representing NM_001033258
  Red=Cloning site Green=Tags(s)
  
  MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTVVKIPPHLLREKCLLPLQLALESKNVKLAQHALA
  GMQKLLSEERFVSMETDSDEKQLLNQILNAVKVTPSLNEDLQVEVMKVLLCITYTPTFDMNGSAVLKIAE
  VCIETYTCSCHQRSINTAVRATLSQMLGDLTLQLRQRQENTIIENPDAPQEFRSQGLTVEALCDDVISVL
  AVLCEKLQASINDSQQLQLLYLECILSVLSSSSSSMHLHRGFTDLIWKSLCPALVVILGNPIHDKTITSA
  HSTSTSTSMESDSASLGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVGSVDSMKPVLQSLYHRV
  LLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSIESEPRKRSISKRKSHLDLLKLIMDGMTEACIKGGI
  EACYAAVSCVCTLLGALDELSQGKGLNDTQVQQLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEH
  TPWESGNERSPDISISVTTDTGQTTLEGELGQTTPEDHKNGLKSPAIQEGKGTMGKVSEPEAIDQPDVVQ
  RSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDN
  CSLADEEQTPRDYIGHRSLRTAALSLKLLKNQEADQHSARLFIQSLEGLLPRLLALSSVEEVDSALQNFA
  STFCSGMMHSPGFDGGSSLSFQMLMNADSLYTAAHCALLLNLKLSHGDYYRKRPTVAPGMMKEFMKQVQT
  SGVLMVFSQAWLEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTDIDGLESSAIGGQLMASASVESPF
  TQSRRLDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMS
  LDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEERQSQEPSDALAQVKLKVEQKLEQMGKVQGVW
  LHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGISQPPLTIHQPQKTSGSSGLLG
  EIEFKSSSQEQSLEQGPSLNTAPVVQPHSIQELVRECSRGRTSDFRGGSLSGNSAAKVVLSLSTQADRLF
  DDATDKLNLTALGGFLYQLKKASQSQLFHSVTDTVDYSLTMPGEVKSTQDQKSALHLFRLGDAMLRIVRS
  KARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQ
  LELCDEDVQDQVVTSIGELVEVCSAQIQSGWRPLFSALETVRSGNKSEVKEYLVGDYSMGKGQAPVFDVF
  EAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGAGATSTDLCLPALDYLRRCSQLLAKI
  YKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVLSDFDDDTGLIEVWIILLEQLTAAVSNCPRQHQPP
  TLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSLWLLRSHKDHSYWDVASANFKHAIGLSCELVVEHIQ
  SFLHSDIRYESMINTMLKDLFELLVVCVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAF
  SATLKPVKDLLGCFHGGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQCSPKTPNNFDH
  AQSCQLIIELPHDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDILLEEFVKGPSPGEEKTVQVPDTKL
  AGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAV
  LTNQETITAEQVKKVLFEEEERSSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNA
  DWVWLVKRLHKLCMELCNHYIQMHLDLESSLEEPLTFKSDPFFILPSFQSESSTPSTGGFSGKNTPSEDD
  RREHLSEPQSLRVGSGDMLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTISKLMTEYKKRRQPHN
  LPPFPKEVKVDKKGEPLGPRGPDSPLLQRPQHLIDQGQMRHSFSAGPELLRQEKRPRSGSTGSSLSVSVR
  DAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIIT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033258
• ORF Size: 6510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033258.4, NP_001028430.1
• RefSeq Size: 8216 bp
• RefSeq ORF: 6513 bp
• Locus ID: 215821
• UniProt ID: Q3UGY8
• Cytogenetics: 10 7.79 cM
• Gene Summary: Participates in the regulation of systemic glucose homeostasis, where it negatively regulates insulin granule biogenesis in pancreatic islet beta cells (PubMed:24711543). Also regulates glucagon granule production in pancreatic alpha cells (PubMed:25737957). Inhibits nuclear translocation of the transcriptional coregulator PHB2 and may enhance estrogen receptor alpha (ESR1) transcriptional activity in breast cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]