Map7 (NM_008635) Mouse Tagged ORF Clone

CAT#: MG217450

Map7 (tGFP-tagged) - Mouse microtubule-associated protein 7 (Mtap7), (10ug)

Product Images

Circular map for MG217450

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Map7
• Synonyms: E-MAP-115; MAP-7; mshi; mste; Mtap7; R75000; ste
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217450 representing NM_008635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCAGGGAGCTGGCGGCGACGGCCACAGGGGCGGCGACGGCGCTACGCACAGCGACCCAGCGT
  CTGATGGCTATAAAGTACAAGAGAAGAGAACTGCCCCCAGCCGCCCCACTTCTACAGTTTCAGGACAAAC
  TAGCAACCACTCAGGAAACAAACCAGATCCCCCACCTGTGCTACGGGTTGATGACCGGCAGCGGCTAGCC
  AGAGAACGCCGAGAGGAACGAGAAAAACAGCTAGCTGCACGAGAAACCGTCTGGCTAGAGAGAGAAGAAC
  GAGCCCGGCAGCACTATGAGAGACATCTGGAGGCGAGGAAGAAGAAGCTGGAGGACCAACGGCTGAAGGA
  GGAGCGGAGGAGGGCTGCGGTGGAGGAGAAGCGGAGGCAGAGGCTGGAGGAGGACAAGGAGCGCCATGAA
  GCTGTTGTCCGACGGACGATGGAAAGGAGCCAGAAGCCAAGGCAGAAGTCTAACCGCTGGTCCTGGGGAA
  GCCCTCTCCATGGGAGCTCGAGCATCCACAGTGGAGATCCAGACAGGCGCTCAGTTTCCACCATGAATCT
  TTCGAAACATGTTGATCCTGTCATTAGCAAGCGGCTCTCCTCCTCGTCTGCAACTTTGCTAAACTCTCCA
  GATAGAGCTCGCCGCCTGCAGCTCAGCCCCTGGGAGAGCAGCGTTGTTAGCAGACTTCTGACGCCCACAC
  ATTCGTTCCTGGCCAGAAGCAAAAGCACGGCCGCCTTGTCTGGAGACACAGCATCTTGCAGCCCCATCAT
  CATGCCCTTCAAAGCTGCACACTCTAGAAACCCAGTGGACCGACCAAAACTCTTTGTAACACCGCCTGAG
  GGCTCTGCACGAAGGAGGACCATTCATGGACTAGCGAGCCATAAAAGAGAGCGAGAAAGAGAACACGTTC
  CCTTCCACGTTTCCCCGGGCGCCCGCAGGACTCTGTCTCCATCTAATCTCAAAGCGAGGTCACCGGCTCC
  AGCCCGCCTTTGGCTCCCATCCAAGTCCATGCCTCATCTGCCTGGTACTCCCCGGCCTGCATCCTCCTTG
  CCTCCCGGCTCAGTCAGAGCTGCTTCCGCTCAGGCCCCCTCCTCCTCTCCTGGCAACATCCGGCCTGTCA
  AGAGAGAAGTGAAAGTGGAGCCTGAGAAGAAAGACCCTTTACCCGCAGTAAAGAGCAGGGTGCCATTAGT
  GAAGGTAGAGGAGGTCACAGTCGAAGAGGGGACACCCGTGAAGCCACCTGAGCCTGCTGCTCCAGCCTCG
  GCCCCCATTGCAACCCCAGCCCCTGCTCCAGCTACGGACCCGGCCCCAGTCCCTGCACCATCATCCACTG
  TGACTGTCGGTGTAGTTCCTAAGACTTCTGCAGGCACCACCGACCCAGAGGAGGCTACGAGGTTGCTGGC
  TGAGAAGAGGCGTCTAGCCAGAGAGCAGAGGGAGAAGGAGGAACGGGAGAGGAAGGAAAAGGAAGAGCTG
  GAGAGACAAAAGATAGAGGAATTGGCCCGTAGGGTGGCTGAAGAGCGAAGTCGCAGGGAAGAAGAAGCCC
  GCAGGCTGGAAGAGGAGCAGGCTCGAGAGAAAGAGGAGCTGGCGCTGCGTCTGGCTGAGGAGGAGCGGGA
  GCGGTGGGAGAGGGAGGAGGTGGAGCGCGTGCAGAAGCAGAAAGAAGAAGAAGCCCGAGCCCGGGAGGAG
  GCAGAGAGGGCTCGGCAGGAACGAGAGAAGCATTTCCAGAAAGAAGAGCAGGAACGGCTGGAGAGGAAGA
  AGCGACTTGAAGAGATTATGAGAAGAACCAGGAGGACAGAGACCGCTGATAAGAAAACCACTGAGCAAAG
  AAATGGTGACATAGCCAAAGGAGTTCTCACTGGAGAGCCAGAAGTACCTGCACTGCCGTGTATGGCCTCT
  TCAGGAAACGGAGAGTCTGCAGAGAGCCCACATGGAGTCGCTTTACAGCAATCAGAAGTGACCACAGAGA
  GTTCTCCAGATTTGGAAAAACAGCCAAATGAAAACGGAATGTCCATACAAAATGAAAATTTTGAAGAAGT
  TATAAACTTACCTGTTGGATCAAAAGCGTCCAGATTAGATGTCACCAATGAGAACCCAGAAATTCCTTTG
  AAACCAATTTTGGCCTTTAATGATGAAGGGACACTTGGGCCCCTACCTCAGGTGGATGGTGTGCAGACAC
  AACAGACCGCAGAAGTTATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217450 representing NM_008635
  Red=Cloning site Green=Tags(s)
  
  MAEQGAGGDGHRGGDGATHSDPASDGYKVQEKRTAPSRPTSTVSGQTSNHSGNKPDPPPVLRVDDRQRLA
  RERREEREKQLAARETVWLEREERARQHYERHLEARKKKLEDQRLKEERRRAAVEEKRRQRLEEDKERHE
  AVVRRTMERSQKPRQKSNRWSWGSPLHGSSSIHSGDPDRRSVSTMNLSKHVDPVISKRLSSSSATLLNSP
  DRARRLQLSPWESSVVSRLLTPTHSFLARSKSTAALSGDTASCSPIIMPFKAAHSRNPVDRPKLFVTPPE
  GSARRRTIHGLASHKREREREHVPFHVSPGARRTLSPSNLKARSPAPARLWLPSKSMPHLPGTPRPASSL
  PPGSVRAASAQAPSSSPGNIRPVKREVKVEPEKKDPLPAVKSRVPLVKVEEVTVEEGTPVKPPEPAAPAS
  APIATPAPAPATDPAPVPAPSSTVTVGVVPKTSAGTTDPEEATRLLAEKRRLAREQREKEERERKEKEEL
  ERQKIEELARRVAEERSRREEEARRLEEEQAREKEELALRLAEEERERWEREEVERVQKQKEEEARAREE
  AERARQEREKHFQKEEQERLERKKRLEEIMRRTRRTETADKKTTEQRNGDIAKGVLTGEPEVPALPCMAS
  SGNGESAESPHGVALQQSEVTTESSPDLEKQPNENGMSIQNENFEEVINLPVGSKASRLDVTNENPEIPL
  KPILAFNDEGTLGPLPQVDGVQTQQTAEVI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008635
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008635.2, NP_032661.2
• RefSeq Size: 3891 bp
• RefSeq ORF: 2193 bp
• Locus ID: 17761
• UniProt ID: O88735
• Cytogenetics: 10 9.75 cM
• Gene Summary: Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.[UniProtKB/Swiss-Prot Function]