Krtap19-5 (NM_010676) Mouse Tagged ORF Clone
CAT#: MG217435
- TrueORF®
Krtap19 (tGFP-tagged) - Mouse keratin associated protein 8-2 (Krtap8-2), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010676" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Krtap19-5 |
Synonyms | Krtap8-2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217435 representing NM_010676
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTACTACGGCAGCTACTACGGAGGTCTGGGCTCTGGCATCCGAGGCTTTGGGAACCTGGGTTATG GCTATGGCTGTGGCTGTGGCTTTGGAGGTTACGGATATGGATCTGGCTATGGCAGATATGGATATGGCTA CCGCCGCCCCCTTTATTATGGAGGATATGGATTCTCCCGATTCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217435 representing NM_010676
Red=Cloning site Green=Tags(s) MSYYGSYYGGLGSGIRGFGNLGYGYGCGCGFGGYGYGSGYGRYGYGYRRPLYYGGYGFSRFY TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010676 |
ORF Size | 186 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010676.2, NP_034806.2 |
RefSeq Size | 482 bp |
RefSeq ORF | 189 bp |
Locus ID | 16704 |
UniProt ID | O08632 |
Cytogenetics | 16 C3.3 |
Gene Summary | In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208859 | Krtap19 (untagged) - Mouse keratin associated protein 8-2 (Krtap8-2), (10ug) |
USD 165.00 |
|
MR217435 | Krtap19 (Myc-DDK-tagged) - Mouse keratin associated protein 8-2 (Krtap8-2) |
USD 150.00 |
|
MR217435L3 | Lenti ORF clone of Krtap19 (Myc-DDK-tagged) - Mouse keratin associated protein 8-2 (Krtap8-2) |
USD 450.00 |
|
MR217435L4 | Lenti ORF clone of Krtap19 (mGFP-tagged) - Mouse keratin associated protein 8-2 (Krtap8-2) |
USD 450.00 |
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