Epc2 (NM_172663) Mouse Tagged ORF Clone

CAT#: MG217228

Epc2 (tGFP-tagged) - Mouse enhancer of polycomb homolog 2 (Drosophila) (Epc2), (10ug)

Product Images

Circular map for MG217228

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Epc2
• Synonyms: 5830499L14; D2Ertd694e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217228 representing NM_172663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAACTCTCCTTCCGAGCGCGGGCGCTGGACGCCGCCAAACCGCTGCCCATCTACCGCGGCAAGG
  ACATGCCTGATCTCAACGACTGCGTCTCCATCAACCGGGCCGTGCCCCAGATGCCCACCGGGATGGAGAA
  GGAGGAGGAATCGGAACATCACCTACAGCGAGCTATTTCAGCGCAGCAAGTATTTAGAGAAAAAAAAGAG
  AGCATGGTCATTCCAGTTCCTGAGGCAGAGAGCAACGTCAACTATTACAATCGCTTGTACAAAGGGGAGT
  TCAAACAGCCCAAGCAGTTCATTCATATTCAGCCTTTTAACCTAGACAACGAGCAACCAGATTATGATAT
  GGATTCAGAAGATGAGACATTATTAAATAGACTTAACAGAAAAATGGAAATTAAACCTTTGCAATTTGAA
  ATTATGATTGACAGACTTGAAAAAGCCAGTTCTAACCAGCTTGTAACACTTCAAGAAGCAAAACTGCTGC
  TAAATGAAGATGATTATCTTATTAAAGCTGTATATGACTACTGGGTGAGAAAACGAAAGAACTGCAGGGG
  GCCATCCCTCATTCCCCAGATTAAGCAAGAGAAAAGAGATGGCTCTACCAACAATGATCCTTACGTTGCC
  TTTCGGAGGAGAACAGAGAAAATGCAGACTCGGAAGAACCGTAAGAATGATGAAGCTTCTTATGAAAAGA
  TGCTGAAGCTGAGGCGGGAGTTCAGCAGAGCCATAACAATTTTGGAAATGATTAAGAGAAGAGAAAAAAC
  CAAACGGGAATTATTGCACTTAACACTGGAAGTTGTGGAAAAAAGATATCATTTGGGAGACTATGGGGGT
  GAAATCCTTAATGAAGTGAAAGTTAATAGATCAGAAAAAGAGTTATATGCCAGTCCAGCAACCCTTCATA
  ATGGAAATCATCACAAAGTTCAAGAATGTAAAACTAAGCACCCTCATCATTTGTCTTTGAAAGAAGAGGC
  TTCTGATGTGGTTCGTCAAAAGAAGAAGTACCCAAAGAAGCCTAAGGCAGAGGCTGGGATAGCCCCTCAG
  CAGCCCACTCCTGAGACATTGCCTGTGATCAATAAGAGTGACATTAAGCAATATGACTTTCAGAGCTCAG
  ACGAAGATGAATTTCCACAGGTGCTGTCTCCAGCTTCAGAGGCTGAGGAGGAGAATGACCCTGACGGTTC
  CTGTGCTTTCAGAAGACGGGCAGGATGCCAGTATTACGCTCCGCGTTTGGACCAAGCTAATAACCATATG
  TGTGAAAATTCAGAGTTGGCAGACTTGGATAAGTTGAGGTATAAGCATTGTCTTACGACACTTACAGTTC
  CAAGAAGATGTATAGGATTTGCAAGGAGGCGAATTGGCAGAGGTGGCAGGGTCATAATGGACCGAATATC
  CACAGAACATGACCCCGTCCTGAAACAGATAGACCCTGAGATGCTGAACGGGTTTTCAAGCTCTTCCCAA
  ACTATAGACTTTTCTTCTAATTTCTCTCGGACCAATGCTTCCAGTAAACCTTGTGAAAATAGACTGTCTC
  TTTCTGAAATATTAAGCAATATCAGATCATGTCGACTACAGTGTTTCCAGCCAAGGCTACTAAATGTACA
  GGACATTGATAGTGAAGAATGTACCTCAAGAAAACCAGGGCAGACTGTGAGCAGTAAAAGAGTTTCTGCA
  GCATCCGTAGCTTTATTGAACACCAGCAAGAACGGCATATCAGTAACAGGGGGCATCACAGAAGAGCAGT
  TTCAGACACATCAACAGCAGCTAGTTCAAATGCAAAGGCAGCAACTTGCGCAGCTTCATCAGAAACAACA
  ATCCCAGCATTCCTCACAGCAGACACATCCAAAAGCACAGGGCTCAAGCACCTCTGACTGTATGTCTAAA
  ACACTTGACTCAGCCAGCGCCCACTTTGCTGCATCTGCAGTGGTCAGTGCTCCTGTTCCAAGTCGCAGTG
  AGGGATCCAAGGAACAGAACACTGGCCACAACAATATGAATGGTGTTGTCCAGCCTTCAGGACCCTCTAA
  AACATTATATTCCACCAATATGGCTTTATCATCCAGCCCAGGGATTTCAGCTGTACAGCTTGTAAGGACA
  GTTGGCCACACTACTACAAACCACTTGATCCCAGCGTTGTGCACAAGCAGTCCTCAGACACTTCCCATGA
  ACAATTCCTGCCTGACCAATGCAGTGCACCTCAATAACGTCAGTGTTGTTTCTCCTGTCAATGTGCATAT
  CAATACACGGACTTCAGCACCATCGCCAACAGCCTTAAAACTTGCCACAGTTGCTGCCAGCATGGACAGA
  GTGCCAAAGGTTACTCCCAGCAGTGCCATCAGCAGCATAGCCAGAGAGAACCATGAACCAGAAAGACTGG
  GTTTAAATGGACTAGCAGAGACAACAGTAGCTATGGAGGTGACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217228 representing NM_172663
  Red=Cloning site Green=Tags(s)
  
  MSKLSFRARALDAAKPLPIYRGKDMPDLNDCVSINRAVPQMPTGMEKEEESEHHLQRAISAQQVFREKKE
  SMVIPVPEAESNVNYYNRLYKGEFKQPKQFIHIQPFNLDNEQPDYDMDSEDETLLNRLNRKMEIKPLQFE
  IMIDRLEKASSNQLVTLQEAKLLLNEDDYLIKAVYDYWVRKRKNCRGPSLIPQIKQEKRDGSTNNDPYVA
  FRRRTEKMQTRKNRKNDEASYEKMLKLRREFSRAITILEMIKRREKTKRELLHLTLEVVEKRYHLGDYGG
  EILNEVKVNRSEKELYASPATLHNGNHHKVQECKTKHPHHLSLKEEASDVVRQKKKYPKKPKAEAGIAPQ
  QPTPETLPVINKSDIKQYDFQSSDEDEFPQVLSPASEAEEENDPDGSCAFRRRAGCQYYAPRLDQANNHM
  CENSELADLDKLRYKHCLTTLTVPRRCIGFARRRIGRGGRVIMDRISTEHDPVLKQIDPEMLNGFSSSSQ
  TIDFSSNFSRTNASSKPCENRLSLSEILSNIRSCRLQCFQPRLLNVQDIDSEECTSRKPGQTVSSKRVSA
  ASVALLNTSKNGISVTGGITEEQFQTHQQQLVQMQRQQLAQLHQKQQSQHSSQQTHPKAQGSSTSDCMSK
  TLDSASAHFAASAVVSAPVPSRSEGSKEQNTGHNNMNGVVQPSGPSKTLYSTNMALSSSPGISAVQLVRT
  VGHTTTNHLIPALCTSSPQTLPMNNSCLTNAVHLNNVSVVSPVNVHINTRTSAPSPTALKLATVAASMDR
  VPKVTPSSAISSIARENHEPERLGLNGLAETTVAMEVT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172663
• ORF Size: 2424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172663.4, NP_766251.3
• RefSeq Size: 4358 bp
• RefSeq ORF: 2427 bp
• Locus ID: 227867
• UniProt ID: Q8C0I4
• Cytogenetics: 2 28.62 cM
• Gene Summary: May play a role in transcription or DNA repair.[UniProtKB/Swiss-Prot Function]