Cox18 (NM_001163456) Mouse Tagged ORF Clone

CAT#: MG217163

Cox18 (tGFP-tagged) - Mouse COX18 cytochrome c oxidase assembly homolog (S. cerevisiae) (Cox18) nuclear gene encoding mitochondrial protein transcript variant 1, (10ug)

Product Images

Circular map for MG217163

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cox18
• Synonyms: BC038311
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217163 representing NM_001163456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGCCGGTCGTGTGCCGGGTGGCTCAGGTCGCTGCCCACCCTGCGGCTCCCGGCACCGAGATCAC
  CGCCCGCCTGGAGCAGCGCGCGGCTCCCAGCCCTTCCGGTATGGGCAGCCGCTTCGGTGTCTGCGGCCTC
  TCCAGGTGGCTGGTATGAGGCCCTGGCAGCGTCGGCGCCGGTGCGCACCGCGGAGGAAGTGCTGCTGGGC
  GCGCAGGAGGCTACCGGCCTCCCCTGGTGGAGCAACATCATACTCAGCACCGTTGCCCTGCGCGGTGCTG
  TCACTCTGCCCCTGGCCGCCTATCAGCACTACATCCTGGCTAAGGTGGAAAACTTGCAACCAGAAATAAA
  AGACATCGCCAAGCGCCTTAACCAAGAAGTTGCAGTCTGTGCCCGTCAGTTTGGATGGTCCAAGAGAGTG
  GCTAGACTCACCTATCTGAAGAACATGCGGAGACTTGTCTCAGAGCTGTATGTCCGTGACAACTGCCACC
  CTTTCAAAGCCACGGTGCTGGTCTGGGTCCAGCTTCCAATGTGGGTTTTCATCTCTGTCGCCCTCCGGAA
  CCTCAGCACTGGGGCCACACATTCAGACGGCATTTCTGTTCAGGAACAGTTAGCTGCTGGCGGGACGCTG
  TGGTTTCCTGACCTCACCGCAGTGGATTCCACCTGGATTCTGCCTGTCTCTGTTGGTGTTGTCAACCTAC
  TGATAGTGGAGATTTTTGCTCTACAAAAAATTGGGACGTCTCGTTTTCAAATGCATGTTACAAATTTTGT
  CCGAGCTGTATCCGTGTTGATGATTCCAGTTGCTGCAACAGTACCCTCGGCGCTTGTTCTCTACTGGCTG
  TGCTCCAGCCTCATGGGCCTCGCACAGAACCTGCTGCTGCGCTCCCCTGGGTTCCGCCAGCTCTGCCGGA
  TACCACCAAGCAAGTCCGACTCAGAGACCCCGTACCGAGACCTCTCTGCTGCCTTTTGCGCCAAGTTCCT
  TTCAAGAAAACGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217163 representing NM_001163456
  Red=Cloning site Green=Tags(s)
  
  MLCRSCAGWLRSLPTLRLPAPRSPPAWSSARLPALPVWAAASVSAASPGGWYEALAASAPVRTAEEVLLG
  AQEATGLPWWSNIILSTVALRGAVTLPLAAYQHYILAKVENLQPEIKDIAKRLNQEVAVCARQFGWSKRV
  ARLTYLKNMRRLVSELYVRDNCHPFKATVLVWVQLPMWVFISVALRNLSTGATHSDGISVQEQLAAGGTL
  WFPDLTAVDSTWILPVSVGVVNLLIVEIFALQKIGTSRFQMHVTNFVRAVSVLMIPVAATVPSALVLYWL
  CSSLMGLAQNLLLRSPGFRQLCRIPPSKSDSETPYRDLSAAFCAKFLSRKR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163456
• ORF Size: 996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163456.1, NP_001156928.1
• RefSeq Size: 1341 bp
• RefSeq ORF: 999 bp
• Locus ID: 231430
• Cytogenetics: 5 E1
• Gene Summary: Mitochondrial membrane insertase required for the translocation of the C-terminus of cytochrome c oxidase subunit II (MT-CO2/COX2) across the mitochondrial inner membrane. Plays a role in MT-CO2/COX2 maturation following the COX20-mediated stabilization of newly synthesized MT-CO2/COX2 protein and before the action of the metallochaperones SCO1/2. Essential for the assembly and stability of the mitochondrial respiratory chain complex IV (also known as cytochrome c oxidase).[UniProtKB/Swiss-Prot Function]