Wdr43 (NM_175639) Mouse Tagged ORF Clone

CAT#: MG217034

Wdr43 (tGFP-tagged) - Mouse WD repeat domain 43 (Wdr43), (10ug)

Product Images

Circular map for MG217034

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wdr43
• Synonyms: 2610318G08Rik; AU020887; AV024208; mKIAA0007; Wrd43
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217034 representing NM_175639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGGAGGCGGCGGTAGCTACGACCCCTTGGCCCCGGCGGGGGTCCCCTGCGCCTTCTCGCCGG
  ACAGTCAAGCCTACTTCGCTTTAGCCTCTAGTGACGGTCAGCTGCGAGTATGGGAGACCGCCAACAACCG
  GCTGCACCAGGAGTACGTGCCTTCCGCGCACCTCAGCGGTACCTGCACCTGCCTGGCCTGGGCGCCAGCA
  CGGCTGCAGGCCAAGGAAAGTCATCAAAGGAAAAAAAGGAAATCGGAAGTTACAGGAACAAAGGACCAGG
  CTGACCTGTTGGCTCTTGGCACGGCAGTTGGTAGCATTTTATTGTATAGTACAGTAAGAGGAGAGTTGCA
  CAGTAAATTAACAAGTGGTGGGCATGAAAACAGAGTGAATTGCATACAGTGGCATCAAGACAATGACTGC
  TTATATAGTTGTTCAGATGATAAGTATATTGTGGAGTGGAGTACACAGACATGCAAAGTAAAGTGCAAAT
  GGAAAGGAGACAATAGCAGCGTCAGTTCCCTGTGTATCAGCCCAGATGGAAAGATGTTACTTTCAGCTGG
  TCGAACAATCAAACTATGGGTTTTGGAGACCAAAGAAGTCTACAGACACTTCACAGGACATGCGACACCT
  GTTTCGTCGCTTAGGTTCACGACCATCAGACCTAATGAGAGCCAGCCTTCTGATGGGATTACAGGCCTTT
  ATTTCTTGTCTGGAGCCGTACACGACCGATTACTTAATGTCTGGCAGGTCCGGTCAGAGAACAAGGAGAA
  GAGTGCAGTGATGTCTTTTACAGTCACAGACGAGCCTGTCTATGTTGACTTGACTTTGTCAGAAAACAAA
  GAAGAGCCTGTCAAGTTAGCTGTTGTTTGCAGAGATGGTCAAGTTCATCTTTTTGAACACATATTAAATG
  GACATTGCAAAAAGCCCTTGACTTCAAATTGCACAATCCAGATCGCAACACCTGGAAAGGGCAAGAAAGT
  AACACCAAAACCCATCCCTATTCTAGCAGCTAGCTTTTGCTTAGACAAAATGTCCTTATTGCTGGTGTAT
  GGAAATTGGTTTCAGCCTACCATTGAGCGAGTGGCTTTGAACTCCAAAGATACTCATATATGTTTGGAAA
  GAGATATTTCAAATTGCTGGGCTCCAACAGTAGAAACTGCTATAACCAAGGTAAAAACACCAGTAATGAA
  TTCTGAAGCAAAAGTTTTGGTGCCCGGGATTCCTGGACATCATGCGCCAATCAAGCTGCCGCCCGCCCAG
  CCCAAGGAGGCCGAGAACAAGAGGAAATTGGGCAGTACTGAGGCTACCATCGAGGAGCGCCTTGGTGCCA
  TGGACCTAGATAGAAAAGGAAGGAAGGACGACCTTCAGACAAACAGCTTTGCAGTCCTCCTTACACAGGG
  CTTAGAAAGCAATGATTTTGAAATATTAAACAAAGTGCTTCAAACTAAGAATGTAAACCTAATAAAGAGG
  ACTGTGCTGAGGATACCGTTGCGTGTTGTTATTCCACTGTTGCAAGAGCTTACGAAGAGGTTACAGGGAC
  ATCCCAATAGTGCTGCTTTAATGATTCAGTGGCTGAAGTGTGTGCTGACGATCCATGCATCATACCTATC
  TACGTTGCCTGACCTAGTAGAGCAGCTGGGAACACTCTACCAGCTGATGGAAAGCAGAGTCAAAACTTTC
  CAGAAACTCTCAAACCTGCATGGAAAACTCATTCTTCTCGTCACGCAAGTTACAGCATCAGAAAAATCGA
  AAAAAATGACCTCTCCCGGACAGAAGGCAAAATTGGTGTATGAAGAAGAGTCTTCTGAGGAGGAGTCTGA
  CGATGAGGTTCCAGAGAAGGATTCTGATGATAATTGGGACGAAGATGAAGACAAAGACAGTGAAAAAGAT
  GAGGGCGTTGATGAAGACAATGAGGAGGAAGATGAGGACATGGAAGACAAAGAAGAGAATGAGGAGGACA
  GAGAAGTGTCAAGTGAAAAAGAATTAAATGGAGATTCGGATTTAGATCCTGAAAACGAGAGTGAAGAAGA
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217034 representing NM_175639
  Red=Cloning site Green=Tags(s)
  
  MAAGGGGSYDPLAPAGVPCAFSPDSQAYFALASSDGQLRVWETANNRLHQEYVPSAHLSGTCTCLAWAPA
  RLQAKESHQRKKRKSEVTGTKDQADLLALGTAVGSILLYSTVRGELHSKLTSGGHENRVNCIQWHQDNDC
  LYSCSDDKYIVEWSTQTCKVKCKWKGDNSSVSSLCISPDGKMLLSAGRTIKLWVLETKEVYRHFTGHATP
  VSSLRFTTIRPNESQPSDGITGLYFLSGAVHDRLLNVWQVRSENKEKSAVMSFTVTDEPVYVDLTLSENK
  EEPVKLAVVCRDGQVHLFEHILNGHCKKPLTSNCTIQIATPGKGKKVTPKPIPILAASFCLDKMSLLLVY
  GNWFQPTIERVALNSKDTHICLERDISNCWAPTVETAITKVKTPVMNSEAKVLVPGIPGHHAPIKLPPAQ
  PKEAENKRKLGSTEATIEERLGAMDLDRKGRKDDLQTNSFAVLLTQGLESNDFEILNKVLQTKNVNLIKR
  TVLRIPLRVVIPLLQELTKRLQGHPNSAALMIQWLKCVLTIHASYLSTLPDLVEQLGTLYQLMESRVKTF
  QKLSNLHGKLILLVTQVTASEKSKKMTSPGQKAKLVYEEESSEEESDDEVPEKDSDDNWDEDEDKDSEKD
  EGVDEDNEEEDEDMEDKEENEEDREVSSEKELNGDSDLDPENESEEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175639
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175639.1, NP_783570.1
• RefSeq Size: 3450 bp
• RefSeq ORF: 2034 bp
• Locus ID: 72515
• UniProt ID: Q6ZQL4
• Cytogenetics: 17 E1.3
• Gene Summary: Ribosome biogenesis factor. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I.[UniProtKB/Swiss-Prot Function]