Plekha6 (NM_001160268) Mouse Tagged ORF Clone

CAT#: MG217000

Plekha6 (tGFP-tagged) - Mouse pleckstrin homology domain containing family A member 6 (Plekha6) transcript variant 2, (10ug)

Product Images

Circular map for MG217000

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plekha6
• Synonyms: 9530028L01Rik; AU042621; Pepp3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217000 representing NM_001160268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAACAAAACAGGTGGGAAACGTTCTGCTACCATCAACAGCGACATAGCCAACCACAACATGGTGT
  CTGAGGTTCCTCCAGAGCGGCCCAACATCCGGGCGACTCGAACTTCCCGAAAAGCCATTGCCTTTGGTAA
  ACGCGCACACTCCATGAAGCGGAACCCCAATGCACCTGTTACCAAGGCGGGCTGGCTCTATAAGCAGGCC
  AGCTCCGGGGTGAAGCAGTGGAATAAGCGTTGGTTTGTCCTGGTGGATCGCTGCCTCTTTTACTACAAAG
  ATGAGAAGCAGGAGAGCATCCTGGGCAGCATCCCCCTGCTGAGCTTCCGGGTGGCGGCTGTGCAGCCTTC
  AGACAACATCAGTCGGAAGCACACGTTTAAGGTGACTGTTCACTGGGTAGATGAGGCTGGGGCCAGTTCT
  ACGCACTGCCTCTCCCCACAGGCAGAGCACGCTGGAGTCCGCACCTACTTCTTCAGTGCCGAGAGCCCCG
  AGGAGCAGGAGGCCTGGATCCAGGCCATGGGCGAGGCTGCTCGGGTCCAGATCCCTCCAGCCCAGAAGTC
  AGTGCCTCAGCCTGTGCGTCACAGCCTCGAGAAGCCAGACTCAGAGAACATCCCACCCAGCAAACACCAT
  CAGCAGCCACCTCACAACAATCTCACCAAGCTGGAGCCTGAGGCCAAGACTCGAGGGGAGGGCGACGGCA
  GGGGCTGTGAGAAGGCCGAGCGCAGGCCTGAGAGGCCTGAAGTCAAGAAAGAGACTTTGGTAAAAGCCAA
  TGGCCTCCCGTCTGGACCTGAGACAGCCTCAGAGCCTGGCAGCCCTTACCCCGATGGCCCGAGGGTTCCA
  GGAGGTGGGGAACACCCTGCTCAGCCCAATGGCTGGCAGTATAGCTCCCCAAGCCGACCAGGGAGCACAG
  CTTTTCCACCTCATGATGGGGACTCTGGGGGACAGCGGCGGAGCTTTCCACCACGTACTGACCCTGACAA
  AATTGCCCAGCGCAAAAGCTCTATGAACCAGCTTCAGCAGTGGGTGAACCTGCGCCGAGGGGTGCCCCCA
  CCCGAAGACCTTCGGAGCCCTTCAAGGTTCTACCCTATGCCCCGCCGGGTCCCTGACTATTACAATCCCT
  ACTCCTCCCAGTATCCTGATGATTACCAGTACTACCCACCAGGGGTGCGGCCCGACAGCATCTGCTCCAT
  GCCAGCCTACGATAGGATTAGTCCACCCTGGGCCCTGGAGGACAAGCGCCACTCCTTCCGGAATGGGGGT
  GGACCCACTTACCAGCTTCATGAGTGGAAGGAGTCTACCAGCTACGGGCGGCAGGATGGCACCGTTTGGA
  TCCCCAGCCCCTCCCGACAGCCAGTCTTTTATGACGAGCTGGATGCCGCCTCTGGCTCCTTGCGCCGCCT
  GTCCTTGCAGCCCCGGTCCCACTCTGTGCCCCGCTCGCCCAGCCAGGGCTCCTACAGCCGTGCCCGCATC
  TACTCCCCAGTGCGCTCACCTAGCGCCCGTTTTGATCGGCTGCCACCACGCAGTGAGGACATCTATGCAG
  ATCCCGCTGCCTACGTGATGAGGCGATCTATCAGCTCCCCAAAGGTCCCTGCATACCCAGAGGTGTTCCG
  GGATGGCCTCCACACCTTCAAGCTAAACGAGCAAGACACGGATAAGCTGCTGGGCAAGCTGTGTGAGCAG
  AACAAGGTAGTAAGGGAACAGGAGCGGCTGGTGCAGCAGCTGCGGGCAGAGAAGGAGAGCCTAGAAAGTG
  CCTTGATGGGAACCCACCAGGAGCTGGAGATGTTTGGGAGCCAGCCCGCCTACCCAGAGAAGCTGCTGCA
  CAAGAAGGAGTCTCTGCAGAACCAACTTATCAACATCCGGGTGGAGCTGTCACAGGCGACCACGGCACTG
  ACCAACAGTACAGTGGTGTATGAGAACCTCGAATCGGAGGTCTCTGCCCTGCATGACGAACTCTGGGAGC
  AGCTCAACTTGGATATCCAGAATGAGGTGCTCAATCGGCAAATCCAAAAGGAGATCTGGAGGATCCAGGA
  TGTGATGGAAGGGCTGAGGAAGAACAACCCCTCTCGGGGCACTGACACAGCCAAGCACAGAGGAGGACTT
  GGACCGTCAGCCACCTACAGCTCCAACAGCCCAGCCAGCCCTCTCAGCTCTGCCAGCCTTACCAGTCCCC
  TGAGCCCCTTTTCAATGGTGTCTGGCTCTCAGGGTTCTCCCACTAAGCCAGGGTCCAGTGAGCCCAAAAC
  AGGCTATGAGACAAGCAAGAAAGACCCGAGCCAGACATCACCCCTGGGTACCCCTAGAGATATCAACCTG
  GTACCCACCAGACAAGAGGTGGAGGCAGAGAAGCAGGCAGCTCTCAACAAAGTTGGCATTGTACCCCCTC
  GGACCAAATCTCCTGCTGAGGAGGAGTTGACACCATCAGCAGTGGTGAGAAGGACCACGAATGGACTCAC
  CAATGGCCTCTCCTCACGGCAAGAGCGCCCCAAGAGTGCTGTGTTCTCTGGAGAGGGCAAGGTGAAAATG
  AGTGTCGAGGAGCAGATGGACCGGATGAGGCGGCATCAGAGCGGTTCCATGAAGGAAAAGCGGAGGAGCC
  TACAGCTCCCAGCCAGCCCAGCCCCTGAGCCCAGCACCCGGCCTGCCTACAAAGTGGTGCGCCGTCACCG
  CAGCATCCATGAAGTAGACATCTCCAACCTGGAGGCAGCCCTGAGAGCCGAGGAACCTGGTGGTCAGGCA
  TATGAGACACCACGGGAGGAGATCGCGAGGCTCCGCAAGATGGAGCTGGAGCCTCAGCACTACGATGTGG
  ACATCAGTAAGGAGCTCTCTACTCCAGACAAAGTCCTCATCCCTGAACGGTACATTGACCTGGAACCTGA
  CACTCCCTTGAGCCCGGAGGAGTTGAAGGAGAAGCAGAAGAAGGTGGAGAGGATCAAGACGCTCATTGCC
  AAATCCAGTATGCAGAACGTGGTGCCCATCGGCGAGGGGGACTCTGTGGACGTGCCCCAGGACTCAGAGA
  GCCAGCTGCAGGAGCAGGAGAAGCGGATTGAAATCTCCTGTGCCCTGGCGACCGAGGCCTCCCGCAGGGG
  CCGCATGCTGTCTGTGCAGTGTGCCACCCCCAGCCCTCCCACCTCCCCTGCTTCCCCGACTCCACCAGTC
  AACCCCCTCTCGTCTGACCGCCCACGGGGCGCCGACAGCAGCCATACCATGCGGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217000 representing NM_001160268
  Red=Cloning site Green=Tags(s)
  
  MSNKTGGKRSATINSDIANHNMVSEVPPERPNIRATRTSRKAIAFGKRAHSMKRNPNAPVTKAGWLYKQA
  SSGVKQWNKRWFVLVDRCLFYYKDEKQESILGSIPLLSFRVAAVQPSDNISRKHTFKVTVHWVDEAGASS
  THCLSPQAEHAGVRTYFFSAESPEEQEAWIQAMGEAARVQIPPAQKSVPQPVRHSLEKPDSENIPPSKHH
  QQPPHNNLTKLEPEAKTRGEGDGRGCEKAERRPERPEVKKETLVKANGLPSGPETASEPGSPYPDGPRVP
  GGGEHPAQPNGWQYSSPSRPGSTAFPPHDGDSGGQRRSFPPRTDPDKIAQRKSSMNQLQQWVNLRRGVPP
  PEDLRSPSRFYPMPRRVPDYYNPYSSQYPDDYQYYPPGVRPDSICSMPAYDRISPPWALEDKRHSFRNGG
  GPTYQLHEWKESTSYGRQDGTVWIPSPSRQPVFYDELDAASGSLRRLSLQPRSHSVPRSPSQGSYSRARI
  YSPVRSPSARFDRLPPRSEDIYADPAAYVMRRSISSPKVPAYPEVFRDGLHTFKLNEQDTDKLLGKLCEQ
  NKVVREQERLVQQLRAEKESLESALMGTHQELEMFGSQPAYPEKLLHKKESLQNQLINIRVELSQATTAL
  TNSTVVYENLESEVSALHDELWEQLNLDIQNEVLNRQIQKEIWRIQDVMEGLRKNNPSRGTDTAKHRGGL
  GPSATYSSNSPASPLSSASLTSPLSPFSMVSGSQGSPTKPGSSEPKTGYETSKKDPSQTSPLGTPRDINL
  VPTRQEVEAEKQAALNKVGIVPPRTKSPAEEELTPSAVVRRTTNGLTNGLSSRQERPKSAVFSGEGKVKM
  SVEEQMDRMRRHQSGSMKEKRRSLQLPASPAPEPSTRPAYKVVRRHRSIHEVDISNLEAALRAEEPGGQA
  YETPREEIARLRKMELEPQHYDVDISKELSTPDKVLIPERYIDLEPDTPLSPEELKEKQKKVERIKTLIA
  KSSMQNVVPIGEGDSVDVPQDSESQLQEQEKRIEISCALATEASRRGRMLSVQCATPSPPTSPASPTPPV
  NPLSSDRPRGADSSHTMRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160268
• ORF Size: 3207 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160268.1, NP_001153740.1
• RefSeq Size: 7085 bp
• RefSeq ORF: 3210 bp
• Locus ID: 240753
• Cytogenetics: 1 E4