Idh3g (NM_008323) Mouse Tagged ORF Clone

CAT#: MG216960

Idh3g (tGFP-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+) gamma (Idh3g) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG216960

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Idh3g
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216960 representing NM_008323
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGAAGGTGGCGATAGCTGCTGGCGGTGCTGCAAAGGCAATGCTCAAGCCAACTCTCCTCTGCC
  GTCCTTGGGAGGTTCTGGCTGCCCATGTGGCCCCCCGAAGGAGCATTTCCTCACAACAAACAATTCCTCC
  ATCTGCTAAGTATGGTGGGCGGCATACAGTGACTATGATCCCAGGGGATGGCATCGGCCCAGAGCTCATG
  TTGCATGTTAAGTCTGTATTCAGGCATGCATGTGTGCCGGTGGACTTTGAAGAGGTGCATGTAAGCTCCA
  ACGCTGATGAGGAGGACATCCGCAATGCCATCATGGCCATCCGCCGGAACCGTGTGGCCCTGAAGGGCAA
  CATTGAAACAAATCATAACCTGCCACCATCCCACAAATCTCGAAACAACATCCTTCGCACCAGCCTAGAC
  CTCTATGCCAACGTCATCCACTGTAAGAGCCTGCCAGGAGTGGTGACCCGGCACAAGGACATAGACATCC
  TCATTGTACGGGAAAACACAGAAGGCGAGTACAGCAGCCTGGAGCATGAGAGCGTAGCAGGAGTGGTGGA
  GAGCTTGAAGATTATCACCAAAGCCAAGTCCCTGCGCATTGCTGAATATGCTTTCAAGCTGGCCCAGGAG
  AGTGGGCGTAAGAAAGTGACGGCTGTGCACAAGGCCAACATCATGAAACTGGGTGATGGACTCTTCCTCC
  AGTGCTGCAGGGAAGTAGCAGCCCACTACCCTCAGATCACCTTTGACAGCATGATTGTAGACAACACAAC
  AATGCAGCTGGTATCCCGGCCTCAGCAGTTTGATGTCATGGTGATGCCTAATCTCTATGGTAACATTGTC
  AACAACGTCTGTGCAGGGCTAGTTGGAGGCCCAGGCCTTGTGGCTGGGGCCAACTATGGCCATGTGTATG
  CAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTGCCAATAAGAACATTGCTAACCCGACTGC
  CACACTGCTAGCAAGCTGCATGATGCTAGACCACCTCAAGCTCCACTCCTATGCCACTTCCATCCGCAAA
  GCTGTCTTAGCATCCATGGACAATGAAAATATGCATACCCCAGATATTGGAGGCCAGGGCACCACATCCC
  AAGCCATCCAGGACATCATTCGTCATATCCGCATCATTAATGGACGGGCTGTGGAGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216960 representing NM_008323
  Red=Cloning site Green=Tags(s)
  
  MALKVAIAAGGAAKAMLKPTLLCRPWEVLAAHVAPRRSISSQQTIPPSAKYGGRHTVTMIPGDGIGPELM
  LHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLD
  LYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE
  SGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV
  NNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRK
  AVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRIINGRAVEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008323
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008323.1, NP_032349.1
• RefSeq Size: 1335 bp
• RefSeq ORF: 1182 bp
• Locus ID: 15929
• UniProt ID: P70404
• Cytogenetics: X 37.41 cM
• Gene Summary: Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.[UniProtKB/Swiss-Prot Function]