Polg2 (NM_015810) Mouse Tagged ORF Clone

CAT#: MG216884

Polg2 (tGFP-tagged) - Mouse polymerase (DNA directed) gamma 2 accessory subunit (Polg2) nuclear gene encoding mitochondrial protein transcript variant 1, (10ug)

Product Images

Circular map for MG216884

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polg2
• Synonyms: mtPolB; p55; polG-beta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216884 representing NM_015810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGCGGTGGCGGCGCCAGGGCCTGCCGCAGGGCCTGCAGGTGCTGGCTGTCCGGGTACGCGGGTC
  CCGCAGATGGGACGCAGCAGCCTGACGCCCCCGAGCATGCGGTGGCCCGCGAGGCGCTGGTAGACCTCTG
  CCGGAGGAGGCACTTTCTCAGCGGGACGCCGCAGCAGCTCAGCACGGCTGCTCTGCTGAGCGGGTGCCAC
  GCGCGCTTTGGACCTTTGGGTGTAGAGTTACGAAAGAACCTAGCCTCACAGTGGTGGTCCTCTATGGTGG
  TGTTCAGGGAGCAGGTCTTCGCGGTGGACTCCCTCCACCAGGAGCCGGGCTCTTCGCAGCCGAGGGACAG
  TGCCTTCAGGTTAGTCTCTCCAGAAAGTATACGCGAAATCTTGCAAGACAGAGAGCCGAGTAAGGAACAG
  CTAGTGGCATTTCTTGAGAACTTATTAAAAACTTCTGGGAAACTACGGGCGACTCTTCTTCACGGTGCCT
  TGGAGCACTATGTTAATTGCCTGGATCTGGTAAACAGAAAGCTACCTTTCGGTCTTGCTCAGATTGGAGT
  CTGTTTCCATCCTGTTTCGAACAGCAATCAGACACCCAGCAGTGTTACAAGAGTTGGTGAAAAGACGGAA
  GCTTCCTTGGTATGGTTTACTCCCACAAGGACTTCGAGCCAGTGGCTTGATTTCTGGTTGCGTCATCGGC
  TTCTGTGGTGGAGAAAGTTTGCTATGAGTCCCTCTAACTTCAGCAGCGCTGATTGTCAGGATGAATTGGG
  ACGCAAGGGAAGCAAACTTTACTACAGCTTCCCCTGGGGAAAGGAGCCAATAGAAACCCTGTGGAACCTA
  GGAGATCAAGAACTCCTACACACATATCCTGGAAATGTGTCTACGATACAGGGTCGAGATGGGCGGAAAA
  ACGTGGTTCCTTGCGTTCTGTCTGTAAGTGGGGACGTAGACCTGGGCACGCTGGCTTACCTCTACGATTC
  CTTCCAGCTGGCAGAGAACTCCTTTGCCAGGAAGAAGAGCCTCCAGAGAAAGGTGCTTAAACTTCACCCT
  TGTTTAGCCCCTATTAAGGTCGCTTTGGATGTGGGGAAAGGCCCAACGGTAGAACTCCGACAGGTTTGTC
  AAGGGCTACTTAATGAGTTACTAGAAAATGGGATTTCTGTGTGGCCTGGCTATTCGGAAACTGTCCATTC
  CTCATTAGAGCAACTCCATTCAAAGTACGATGAGATGAGTGTCCTCTTCTCAGTTCTGGTCACTGAGACT
  ACGCTGGAAAATGGGCTAATCCAGCTGCGGAGCAGAGACACCACGATGAAGGAAATGATGCACATATCCA
  AGCTGAGAGACTTCCTGGTCAAGTACCTAGCGTCAGCTAGCAATGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216884 representing NM_015810
  Red=Cloning site Green=Tags(s)
  
  MRCGGGARACRRACRCWLSGYAGPADGTQQPDAPEHAVAREALVDLCRRRHFLSGTPQQLSTAALLSGCH
  ARFGPLGVELRKNLASQWWSSMVVFREQVFAVDSLHQEPGSSQPRDSAFRLVSPESIREILQDREPSKEQ
  LVAFLENLLKTSGKLRATLLHGALEHYVNCLDLVNRKLPFGLAQIGVCFHPVSNSNQTPSSVTRVGEKTE
  ASLVWFTPTRTSSQWLDFWLRHRLLWWRKFAMSPSNFSSADCQDELGRKGSKLYYSFPWGKEPIETLWNL
  GDQELLHTYPGNVSTIQGRDGRKNVVPCVLSVSGDVDLGTLAYLYDSFQLAENSFARKKSLQRKVLKLHP
  CLAPIKVALDVGKGPTVELRQVCQGLLNELLENGISVWPGYSETVHSSLEQLHSKYDEMSVLFSVLVTET
  TLENGLIQLRSRDTTMKEMMHISKLRDFLVKYLASASNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015810
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015810.2, NP_056625.2
• RefSeq Size: 1585 bp
• RefSeq ORF: 1380 bp
• Locus ID: 50776
• UniProt ID: Q9QZM2
• Cytogenetics: 11 E1
• Gene Summary: Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA.[UniProtKB/Swiss-Prot Function]