Rspo2 (NM_172815) Mouse Tagged ORF Clone

CAT#: MG216699

Rspo2 (tGFP-tagged) - Mouse R-spondin 2 homolog (Xenopus laevis) (Rspo2), (10ug)

Product Images

Circular map for MG216699

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rspo2
• Synonyms: 2610028F08Rik; AA673245; D430027K22; ftls
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216699 representing NM_172815
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTTTTGCCTCTTCTCATTTGCCCTCATCATTCTGAACTGTATGGATTACAGCCAGTGCCAAGGCA
  ACCGATGGAGACGCAATAAGCGAGCTAGTTATGTATCAAATCCCATTTGCAAGGGTTGTTTGTCTTGTTC
  GAAGGACAATGGTTGCAGCCGATGTCAACAGAAGTTGTTCTTTTTCCTTCGAAGAGAAGGAATGCGTCAG
  TATGGAGAGTGCCTGCATTCCTGCCCATCAGGGTATTATGGACACCGAGCCCCAGATATGAACAGATGTG
  CACGATGCAGAATAGAAAACTGTGATTCTTGCTTTAGCAAAGACTTTTGTACGAAGTGCAAAGTAGGCTT
  TTATTTGCATAGAGGCCGCTGCTTTGATGAATGTCCAGATGGTTTTGCACCGTTAGATGAGACTATGGAA
  TGTGTAGAAGGTTGTGAAGTTGGTCATTGGAGCGAATGGGGAACGTGTAGCAGAAACAACCGCACGTGTG
  GATTTAAATGGGGTCTGGAAACCAGAACACGGCAGATTGTTAAAAAGCCAGCAAAAGACACAATACCATG
  TCCGACCATTGCGGAGTCCAGGAGATGCAAGATGGCCATGAGGCACTGTCCAGGAGGAAAGAGAACACCA
  AAGGCAAAAGAGAAGAGAAACAAGAAGAAGAGGCGGAAGCTGATTGAGAGAGCCCAAGAGCAGCACAGCG
  TCTTCCTCGCTACAGACAGAGTGAACCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216699 representing NM_172815
  Red=Cloning site Green=Tags(s)
  
  MRFCLFSFALIILNCMDYSQCQGNRWRRNKRASYVSNPICKGCLSCSKDNGCSRCQQKLFFFLRREGMRQ
  YGECLHSCPSGYYGHRAPDMNRCARCRIENCDSCFSKDFCTKCKVGFYLHRGRCFDECPDGFAPLDETME
  CVEGCEVGHWSEWGTCSRNNRTCGFKWGLETRTRQIVKKPAKDTIPCPTIAESRRCKMAMRHCPGGKRTP
  KAKEKRNKKKRRKLIERAQEQHSVFLATDRVNQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172815
• ORF Size: 729 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172815.3, NP_766403.1
• RefSeq Size: 3340 bp
• RefSeq ORF: 732 bp
• Locus ID: 239405
• UniProt ID: Q8BFU0
• Cytogenetics: 15 16.73 cM
• Gene Summary: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors (PubMed:21693646). During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form (By similarity).[UniProtKB/Swiss-Prot Function]