Pde9a (NM_001163748) Mouse Tagged ORF Clone

CAT#: MG216600

Pde9a (tGFP-tagged) - Mouse phosphodiesterase 9A (Pde9a) transcript variant 2, (10ug)

Product Images

Circular map for MG216600

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde9a
• Synonyms: PDE9A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216600 representing NM_001163748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCGGCTCCTCAAGCTACCGGCCCAAGGCCATCTACCTAGACATCGATGGACGCATCCAGAAGG
  TGGTTTTCAGCAAGTACTGCAACTCCAGTGACATCATGGACCTGTTCTGTATCGCCACCGGCCTGCCTCG
  GAACACCACCATCTCCCTTTTAACCACAGACGACGCCATGGTCTCCATCGATCCCACCATGCCTGCGAAT
  TCAGAGCGCACTCCCTACAAGGTGAGACCTGTGGCTGTGAAGCAAGTGTCTGAACGAGAAGAACTTATCC
  AGGGCGTGCTGGCCCAGGTGGCGGAACAATTTTCCAGAGCGTTTAAGATCAACGAGCTGAAAGCCGAAGT
  TGCAAATCACCTGGCCGTGCTGGAGAAACGGGTGGAATTGGAAGGACTTAAAGTGGTGGAGATCGAAAAA
  TGCAAGAGTGACATTAAAAAGATGCGGGAGGAGTTGGCAGCTAGGAACAGCAGGACCAACTGTCCATGTA
  AATACAGTTTTTTGGATAACAAGAAGTTGACACCTCGACGTGATGTCCCCACTTACCCCAAGTACCTGCT
  CTCCCCAGAGACCATCGAAGCCCTACGGAAGCCCACCTTTGATGTCTGGCTTTGGGAACCCAACGAGATG
  CTGAGCTGTTTAGAACATATGTACCACGACCTTGGTCTGGTCAGGGACTTCAGCATCAACCCAATCACGC
  TCCGCAGGTGGCTGCTCTGTGTGCATGACAACTACAGGAACAACCCCTTCCACAACTTCCGGCACTGCTT
  CTGTGTGACACAGATGATGTACAGTATGGTCTGGCTCTGTGGCCTCCAGGAGAAGTTTTCCCAGATGGAC
  ATCTTGGTCCTGATGACTGCAGCCATCTGCCATGACCTGGACCACCCAGGGTACAACAATACATACCAGA
  TCAATGCCCGCACGGAACTCGCTGTGCGCTACAACGACATCTCACCGCTGGAGAACCACCACTGCGCCAT
  TGCCTTCCAGATCCTGGCAAGACCTGAGTGCAACATCTTCGCCAGTGTGCCACCCGAGGGCTTCAGGCAG
  ATCAGGCAGGGGATGATCACATTGATCCTGGCTACCGACATGGCAAGGCATGCTGAAATTATGGATTCTT
  TCAAAGAAAAGATGGAGAATTTTGACTACAGCAACGAGGAGCACCTGACCCTGCTGAAGATGATTCTCAT
  AAAATGCTGTGATATCTCCAATGAAGTCCGTCCCATGGAGGTGGCAGAACCGTGGGTGGACTGTTTACTG
  GAAGAATATTTTATGCAGAGTGACCGTGAGAAGTCCGAAGGCCTTCCTGTGGCCCCATTCATGGACCGAG
  ACAAAGTGACCAAAGCAACAGCCCAAATTGGGTTCATCAAGTTTGTCCTGATCCCAATGTTTGAAACAGT
  GACCAAGCTCTTCCCCGTTGTTGAGGAGACCATGCTGCGGCCGCTCTGGGAGTCCCGAGAACACTACGAG
  GAGCTGAAGCAGCTGGACGATGCCATGAAGGAGTTGCAGAAGAAGACAGAGAGCTTAACATCTGGGGCCC
  CGGAAAACACCACAGAGAAGAACAGAGATGCAAAAGACAGTGAAGGCCATTCTCCTCCAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216600 representing NM_001163748
  Red=Cloning site Green=Tags(s)
  
  MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPAN
  SERTPYKVRPVAVKQVSEREELIQGVLAQVAEQFSRAFKINELKAEVANHLAVLEKRVELEGLKVVEIEK
  CKSDIKKMREELAARNSRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEM
  LSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRNNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMD
  ILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQ
  IRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLL
  EEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPVVEETMLRPLWESREHYE
  ELKQLDDAMKELQKKTESLTSGAPENTTEKNRDAKDSEGHSPPN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163748
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163748.1, NP_001157220.1
• RefSeq Size: 2061 bp
• RefSeq ORF: 1605 bp
• Locus ID: 18585
• UniProt ID: O70628
• Cytogenetics: 17 B1
• Gene Summary: Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes (PubMed:9624145). Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (PubMed:22328573, PubMed:24746365).[UniProtKB/Swiss-Prot Function]