Pum1 (NM_030722) Mouse Tagged ORF Clone

CAT#: MG216598

Pum1 (tGFP-tagged) - Mouse pumilio 1 (Drosophila) (Pum1) transcript variant 1, (10ug)

Product Images

Circular map for MG216598

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pum1
• Synonyms: AA517475; mKIAA0099; Pumm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216598 representing NM_030722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCCCACCTGAAAC
  ATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCTGGAACAGGGTCGCAAGCGCA
  GCCACAGCCAGCTGCAAATCAGGCTCTTGCAGCTGGAACACACTCCAGCCCTGTCCCAGGATCCATAGGA
  GTTGCAGGCCGTTCCCAGGACGACGCTATGGTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTG
  GGGGAGGAGGAAGTGGTGGAGGCGGCTATAATACTAGCAAACATCGATGGCCTACAGGGGATAACATTCA
  TGCAGAACACCAGGTACGCTCTATGGATGAATTGAATCATGATTTTCAAGCACTTGCTCTGGAAGGGAGA
  GCTATGGGCGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAAGATGGACCAA
  AAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCAGATCATTCAGTTTCCCAGCC
  AATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTGAACAGTGAGGTCAATTCTGTACTGTCCCCA
  CGGTCGGAGAGTGGGGGACTAGGCGTTAGCATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGACTCCT
  GTCTAAGAAAAGGAGGATTTGGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAA
  CAAGGGTACGTTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGAGATGTGATGGACAAGACCAAT
  GGTTTGCCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGTAATTGCCAGA
  ACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAGGGCTTAGCCCAGCTGACCAG
  CACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATGGAGTCCCAGAGTGTCCCCTTGGACCCCATG
  GAACATGTGGGCATGGAGCCTCTGCAGTTTGATTATTCAGGCACGCAGGTACCTGTGGACTCTGCAGCAG
  CAACTGTGGGACTTTTTGACTACAATTCTCAACAACAGCTATTCCAAAGACCCAATGCACTTGCTGTTCA
  GCAGTTGACAGCTGCCCAGCAGCAGCAGTATGCACTTGCAGCAGCCCACCAGCCTCACATCGGTTTAGCT
  CCCGCTGCGTTTGTCCCCAACCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCCTACACAGCTG
  GATTGGCTGCAGCAGCGACACTCGGCCCAGCTGTGGTCCCTCACCAATATTATGGAGTCACGCCATGGGG
  TGTCTACCCTGCCAGTCTCTTCCAGCAGCAGGCAGCTGCAGCTGCAGCAGCTACAAACTCTGCTACTCAG
  CAGAGCGCTCCGCAGGCCCAGCAGGGACAGCAGCAGGTTCTTCGTGGAGGAGCTAGCCAACGTCCTTTGA
  CCCCAAATCAGAACCAGCAGGGACAACAAACAGATCCCCTTGTAGCAGCTGCAGCAGTGAATTCTGCTCT
  TGCCTTTGGACAAGGATTGGCTGCAGGTATGCCAGGTTATCCAGTCTTGGCACCTGCTGCTTACTATGAC
  CAAACTGGTGCCCTTGTGGTGAATGCAGGGGCAAGAAATGGCCTCGGAGCTCCAGTCCGGCTTGTTGCTC
  CAGCCCCAGTCATCATTAGCTCCTCAGCTGCACAAGCAGCTGTTGCAGCGGCTGCAGCGTCAGCAAACGG
  AGCAGCTGGTGGTCTTGCCGGAACTACAAATGGACCATTTCGACCTTTAGGCACCCAGCAGCCCCAGCCC
  CAGCCTCAGCAGCAGCCCAGTAACAATCTGGCTTCCAGCTCTTTCTATGGCAACAACTCTCTGAGCAGTA
  ACTCCCAGAGCAGCTCCCTCTTCTCTCAGGGCTCTGCACAGCCTGCCAACACGTCCTTGGGATTCGGAAG
  CAGCAGTTCCCTTGGTGCCACCCTGGGATCGGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAAC
  ACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACATCGAGCAGCT
  TGGCCCCCATTGGACACAGTTTTTATAGCAGCCTTAGCTATTCCTCCTCTCCTGGACCCGTGGGCATGCC
  TCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCTCCTTCCCTCTCTTCACATGGATCCTCTTCA
  AGCTTAAACCTGGGAGGACTCACAAACGGCAGTGGAAGATACATCTCTGCTGCTCCCGGTGCCGAAGCCA
  AGTACCGCAGTGCAAGCAGTGCCTCCAGCCTTTTCAGCCCCAGCAGCACCCTTTTTTCTTCCTCTCGTTT
  GCGATATGGAATGTCTGATGTTATGCCCTCTGGCAGGAGCAGACTTCTGGAAGATTTTCGAAACAACCGG
  TACCCTAATTTACAACTGCGGGAGATTGCTGGGCATATAATGGAATTTTCTCAAGACCAGCATGGCTCTA
  GATTCATTCAGCTGAAACTAGAGCGTGCCACAGCAGCTGAGCGCCAGCTTGTCTTCAATGAAATCCTCCA
  GGCTGCGTACCAACTTATGGTGGATGTGTTTGGAAATTACGTCATTCAGAAGTTTTTTGAATTTGGCAGT
  CATGAACAGAAGCTGGCTCTGGCTGAACGGATTCGAGGCCATGTCCTATCATTGGCACTGCAGATGTACG
  GCTGCCGAGTCATCCAGAAAGCTTTGGAGTTTATTCCCTCAGACCAGCAGAACGAGATGGTGCGGGAGCT
  AGATGGCCATGTCCTGAAGTGTGTGAAAGACCAGAACGGCAACCATGTGGTTCAGAAGTGCATTGAGTGT
  GTACAGCCCCAGTCTTTGCAGTTTATCATCGATGCATTCAAGGGGCAGGTGTTTGCTTTGTCTACGCACC
  CGTACGGCTGTAGGGTGATCCAGAGGATCCTAGAGCACTGTCTCCCTGACCAGACCCTCCCCATCCTGGA
  GGAGCTTCACCAGCACACAGAGCAGCTTGTGCAGGATCAGTATGGAAATTATGTAATCCAACATGTACTG
  GAGCATGGTCGTCCTGAGGATAAAAGCAAAATTGTAGCAGAGATCCGAGGCAATGTACTTGTATTGAGTC
  AGCACAAGTTTGCAAGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACAGAGCGTGCTGTGCT
  CATTGATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGATCAGTAT
  GCTAACTATGTGGTCCAGAAGATGATCGATGTGGCTGAGCCGGGTCAGCGGAAGATTGTCATGCACAAGA
  TCCGGCCACACATTGCGACGCTTCGAAAGTACACCTATGGCAAGCACATCCTGGCCAAGCTCGAGAAGTA
  CTACATGAAGAATGGCGTGGACTTGGGGCCCATTTGTGGTCCCCCTAATGGTATCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216598 representing NM_030722
  Red=Cloning site Green=Tags(s)
  
  MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIG
  VAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNTSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGR
  AMGEQLLPGKKFWETDESSKDGPKGIFLGDQWRDSAWGTSDHSVSQPIMVQRRPGQSFHVNSEVNSVLSP
  RSESGGLGVSMVEYVLSSSPGDSCLRKGGFGPRDADSDENDKGEKKNKGTFDGDKLGDLKEEGDVMDKTN
  GLPVQNGIDADVKDFSRTPGNCQNSANEVDLLGPNQNGSEGLAQLTSTNGAKPVEDFSNMESQSVPLDPM
  EHVGMEPLQFDYSGTQVPVDSAAATVGLFDYNSQQQLFQRPNALAVQQLTAAQQQQYALAAAHQPHIGLA
  PAAFVPNPYIISAAPPGTDPYTAGLAAAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSATQ
  QSAPQAQQGQQQVLRGGASQRPLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYD
  QTGALVVNAGARNGLGAPVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQQPQP
  QPQQQPSNNLASSSFYGNNSLSSNSQSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTAVANSN
  TGSGSRRDSLTGSSDLYKRTSSSLAPIGHSFYSSLSYSSSPGPVGMPLPSQGPGHSQTPPPSLSSHGSSS
  SLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFSSSRLRYGMSDVMPSGRSRLLEDFRNNR
  YPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATAAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGS
  HEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQNEMVRELDGHVLKCVKDQNGNHVVQKCIEC
  VQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPILEELHQHTEQLVQDQYGNYVIQHVL
  EHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAVLIDEVCTMNDGPHSALYTMMKDQY
  ANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGII
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030722
• ORF Size: 3567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030722.2, NP_109647.2
• RefSeq Size: 5370 bp
• RefSeq ORF: 3570 bp
• Locus ID: 80912
• UniProt ID: Q80U78
• Cytogenetics: 4 D2.2
• Gene Summary: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3' UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3' UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (By similarity). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3' UTR and facilitating miRNA regulation (By similarity). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (By similarity). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3' UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3' UTR of mRNAs coding for regulators of p53/TP53 (PubMed:22342750). Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (PubMed:24412312). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function]