Ppp1r3a (NM_080464) Mouse Tagged ORF Clone

CAT#: MG216554

Ppp1r3a (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 3A (Ppp1r3a), (10ug)

Product Images

Circular map for MG216554

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppp1r3a
• Synonyms: GM; RG1; RGL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216554 representing NM_080464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCTGAAGAACCTGGTCAGATTAGCAAAGATAACTTTTTAGAAGTTCCTAATCTGTCTGATT
  CTGTGTGTGAGGATGAAGAAGTTAAGGCGACCTTCAAGCCTGGCTTCTCCCCTCAGCCAAGCAGACGCGG
  GTCAGGATCTTCGGAAGACATGTATCTGGACACTCCAACCTCAGCTTCCCGGAGAGTTTCCTTTGCTGAC
  AGCCTGGGATTCAGCCTTGTGTCTGTTAAAGAATTTGATTGCTGGGAACTACCCAGTGTTTCAACTGATT
  TTGACTTAAGTGGGGATGTTTTCCACACAGATGAATATGTTTTATCTCCACTGTTTGACTTGCCTTCTTC
  AAAAGAAAAGCTTATGGAACAACTCCAAGTCCAGAAAGCCGTGCTGGAGTCCGCTGAACACCTTCCCGGG
  TCAAGCATGAAGGGCATTATTCGAGTCCTGAACATTTCTTTTGAGAAGTTAGTGTATGTGCGCATGTCTT
  TGGATGACTGGCAGACACATTATGACATTTTAGCAGAATATGTTCCTAACTCGTGCGACGGTGAGACTGA
  CCAATTCTCTTTTAAGATTTCATTGGTTCCACCTTATCAAAAAGAGGGCGGTAAAGTGGAGTTTTGTATA
  CGCTATGAAACTTCTGCTGGCACATTTTGGTCAAATAATAATGGCACAAATTATATATTGGTTTGTCAAA
  AGAAGAGGAAGGAGCCAGAGCCTGTAAAGCCATTGGAAGAAGCACCTAGCAGACAAATAAAAGGCTGCTT
  AAAGGTAAAATCACGCAGTAAAGAAGAACCATTGCTAGCACCAGAAGAAAATAAATTTGAGACTTTAAAG
  TTCACAGAGTCCTACATCCCAACAATCATTTGTTCTCACGAGGACAAGGATGACTTGGGAGCCAATCATC
  CAAATGTAGACGATATCAACAAGAAGCATGACGAACACAATGGAAAGGAGCTAGACTTGATGATAAACCA
  ACGGTTAATAACTTCCCAGGATGAAAAGAATACATTTGCAACAGATACAGTCAATTTTACAAATAAAGCT
  GAAGGGTCAGAGAAGAAGCAAGCTTACCATGAAATCAACACTGACTTGTTCATGGGGCCTTTGTCTCCAA
  GTTTATCAGCAGAAAGTTCCTTAAAGCGAGATTTCTATCATAGCAGAAGTTCTTCCCCAGGAAATGAATA
  TGGTCATCCACATTCAGAAGAAATTATTTCAGATATGGGAGAAAAAGGTCCATCACTGGGAGACACTAGT
  TCTGATGAGTTAATGCAATTGGAACTCTGCAGCAAGGAAGACCTGGATGATAATGCCAACCCAGCCAATG
  GGAGTGGCAGAGTGTGCTCTTCCTTCGATCAAAGAATGGCATGTGGTCTCAAGAATAATGAAGCAGGAAT
  CAAGAAAACTGGAATACAAGATTACAAATATTCACATGGAGATTCCACAAAATTGGAAGAGTCAAATGCA
  TCTTCCAGAGATGATTATGCCAAAGTCGATAATAAAAAGGAGAAGCAAACATGCTTAGGTGTTAATGAAA
  ACCCAAGCAAGAATTTTCAATCAGTATTCCAGACCCAAGAGGGACACATGGGGTACCCAAAAATAAGCAC
  TGAAGGGGATAAAGCTAATAACCAAGACTTGACAAGTCTACTGAGCAAAGATATTACAGCTAACACCTGG
  GCGGTGACAGTGGATCCCTGTCCTTCCACAAATGCAAAAAGGAGTTGGAGAGAAGTTGGAAGTGGTAGCA
  ATTTAGAACCAGGAACTAGTGATCTTAGTTCTCCAAGGAATTTCAGCCCTCTGACTGATGATCATCTTTT
  TCAAGCTGACAGGGAAAACTCAGACTCTAGCAATCCTGAAAATCAGAATATGAACACACGACATAGAAAA
  AAATGGAACGTTCTGGAAACCCAGTCAGAAACAAGTGAGACTGAATCAGATATAGCCAAGCATACCAAAG
  AACAAGCAGAGTATAAAGACATGTGGGAAAAAACAGATAACAGTAGGAATCTGAAAGCTACTCCTACAGA
  ACACTTGTTTACCTGCCGAGAGACAGAATGCTACGGACTGTCTTCTCTTGCTGATCATGGTATTACTGAG
  AAAGCACAAGCAGTTACAGCTTATATAATTAAGACAACATTAGAAAGTACTCCAGAAAGCGCGTCTGCTA
  GAGGAAAAGCAATAATTGCTAAGCTACCTCAAGAGACAGCAGGAAATGACAGGCCCATCGAGGTAAAAGA
  AACAGCGTTTGATCCACATGAAGGGAGGAAAGATGATTCACATTATTCCCTTTGTCATGGAGATACAGCA
  GGTGTAATCCATGACAATGATTTTGAAAGGGAGTCACATTTAGATATTTGTAATTTACGTGTGGATGAAA
  TGAAGAAGGAGAAAACCACATCTACGTGCTTTCCTCAGAAGACATATGACAAGGAGAAACATGGAATTGG
  AAGTGTAACATCTATAGACGAACCTTCACAGGTCATTACAGGCAATCAAAAAGCTACGTCAAAACTGGAT
  TTACATTTGGGAGTGTTACCAACAGACAGAGCAATATTCCAAGCAAATGCAGATCTTGAGCTGCTTCAAG
  AATTATCAAGGAGAACAGACTTCAACGCCGTTCCCTCTGCATTTAACTCAGACACTGCCAGCGCTTCTCG
  GGACAGCTCTCAAGTTTACAGACACTGCTCTAAAAAATCAGTACCTTCTTACGGTGAAGAGAAAGCAGTT
  ACTAACACAACCCTCCAGTCTATTCCTACCAAATCAGAATATAATTGGCACCCAGAAAGTGAGGTCTTGG
  GCCATGCCATGTCTAAACCTGAAGATGTTTTCAAAAGTTCAGAAATAATGAAATCAGGTAGTGGAGGAGA
  ACGAGGCGGGGGTCCGATTCTCCAACAAAAAGAAGGTAGCTTGGAAAACTCCCAGGGCCCGATGTTTTTT
  ACCAATGAGCCTCTTGAGAATCTGGATGAAGCAAGTTCTGAAAATGAAGGGTTAATGCATTCTGGACAGT
  CACAGTGTTATCTGGGTGACAAAGGATTAGTGAGCTCTGCTTCTGCTACTGTCTCTACGCAGGAACTCGA
  AGCTCAAGGCAGGGAGTCTCTGCTTTCAATATCCACCAACTCTAAAATACCATATTTCCTTTTGTTTCTG
  ATATTTCTTGCAACTGTCTACTACTATGACTTAATGATAGGCTTGGCGTTCTACCTTTTTTCATTATACT
  GGTTGTACTGGGAAGGGGGGAGGCAAAGAGAGTCTGTTAAGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216554 representing NM_080464
  Red=Cloning site Green=Tags(s)
  
  MEPAEEPGQISKDNFLEVPNLSDSVCEDEEVKATFKPGFSPQPSRRGSGSSEDMYLDTPTSASRRVSFAD
  SLGFSLVSVKEFDCWELPSVSTDFDLSGDVFHTDEYVLSPLFDLPSSKEKLMEQLQVQKAVLESAEHLPG
  SSMKGIIRVLNISFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKISLVPPYQKEGGKVEFCI
  RYETSAGTFWSNNNGTNYILVCQKKRKEPEPVKPLEEAPSRQIKGCLKVKSRSKEEPLLAPEENKFETLK
  FTESYIPTIICSHEDKDDLGANHPNVDDINKKHDEHNGKELDLMINQRLITSQDEKNTFATDTVNFTNKA
  EGSEKKQAYHEINTDLFMGPLSPSLSAESSLKRDFYHSRSSSPGNEYGHPHSEEIISDMGEKGPSLGDTS
  SDELMQLELCSKEDLDDNANPANGSGRVCSSFDQRMACGLKNNEAGIKKTGIQDYKYSHGDSTKLEESNA
  SSRDDYAKVDNKKEKQTCLGVNENPSKNFQSVFQTQEGHMGYPKISTEGDKANNQDLTSLLSKDITANTW
  AVTVDPCPSTNAKRSWREVGSGSNLEPGTSDLSSPRNFSPLTDDHLFQADRENSDSSNPENQNMNTRHRK
  KWNVLETQSETSETESDIAKHTKEQAEYKDMWEKTDNSRNLKATPTEHLFTCRETECYGLSSLADHGITE
  KAQAVTAYIIKTTLESTPESASARGKAIIAKLPQETAGNDRPIEVKETAFDPHEGRKDDSHYSLCHGDTA
  GVIHDNDFERESHLDICNLRVDEMKKEKTTSTCFPQKTYDKEKHGIGSVTSIDEPSQVITGNQKATSKLD
  LHLGVLPTDRAIFQANADLELLQELSRRTDFNAVPSAFNSDTASASRDSSQVYRHCSKKSVPSYGEEKAV
  TNTTLQSIPTKSEYNWHPESEVLGHAMSKPEDVFKSSEIMKSGSGGERGGGPILQQKEGSLENSQGPMFF
  TNEPLENLDEASSENEGLMHSGQSQCYLGDKGLVSSASATVSTQELEAQGRESLLSISTNSKIPYFLLFL
  IFLATVYYYDLMIGLAFYLFSLYWLYWEGGRQRESVKKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080464
• ORF Size: 3267 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080464.2, NP_536712.2
• RefSeq Size: 7120 bp
• RefSeq ORF: 3270 bp
• Locus ID: 140491
• UniProt ID: Q99MR9
• Cytogenetics: 6 A1
• Gene Summary: Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase.[UniProtKB/Swiss-Prot Function]