Lmod2 (NM_053098) Mouse Tagged ORF Clone

CAT#: MG216300

Lmod2 (tGFP-tagged) - Mouse leiomodin 2 (cardiac) (Lmod2), (10ug)

Product Images

Circular map for MG216300

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lmod2
• Synonyms: C-Lmod
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216300 representing NM_053098
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACGTTTGGCTACAGAAGGGGACTTAGTAAATATGAATCCATTGATGAGGATGAACTTCTGGCCT
  CCCTCTCACCTGAAGAGCTGAAGGAGCTTGAGAGGGAGCTGGAAGACATTGAACCTGACCGAAACCTTCC
  TGTGGGGCTAAGGCAAAAGAGTCTGACAGAGAAAACCCCAACAGGAAACTTCAGCCGAGAGGCGCTGATG
  GCGTATTGGGAAAAGGAGTCCCAAAAACTTTTGGAGAAGGAACGGCTGGGGGAATGTGGAAAGGTTGCAG
  AAGAAGACAAGGAAGAGAGTGAAGAGGAACTAATTTTCACAGAGAGCAACAGCGAAGTCTCTGAGGAGGT
  GTGTACAGAAGATGAGGAAGAGTCTCAGGAGGAAGAAGAGGATAGCGAGGAAGAAGAGGACAGTGAGGAA
  GAGGAAGAGACAACAGAAGCCACAAAACATATTAATGGAACTGTAAGCTACAATAGTGTTAATACTGATA
  ACTCTAAGCCAAAGACATTTAAAAGCCAAATAGAAAATATAAATTTAACCAACGGCAACAGTGGAAGAAC
  ACAGAGGAACTCCGAGTCACCAGCCGCCATTCACCCTTGTGGAAATCCCACTGTTATTGAGGATGCTCTG
  GAAAAGATTAGAAACAATGACCCTGATACGACAGAAGTTAATCTGAACAATATAGAGAACATCACCACCC
  AGACCTTATCCCGATTTGCTGAAGCTCTCAAGGAGAACACAGTGGTGAAGACGTTCAGCCTGGCCAACAC
  GCACGCTGATGACGCCGCGGCGATTGCCATTGCAGACATGCTCAAGGTTAACGAGCACATTACCAGCGTA
  AACGTGGAGTCCAACTTCATCACTGGCAAGGGGATCCTGGCCATCATGAGAGCCCTGCAGCACAACACGG
  TGCTCACGGAGCTGCGCTTCCACAACCAGCGGCACATCATGGGCTCTCAGGTGGAGATGGAAATTGTCAA
  GCTGCTCAAAGAGAATACCACGCTGCTGAGGCTGGGCTACCATTTTGAGCTCCCAGGACCAAGAATGAGT
  ATGACGAGTATTTTAACAAGGAATATGGATAAACAGAGGCAAAAACGCATGCAGGAGCAAAAACAGCAAG
  AGGGGCACGATGGAGGAGCTGCTCTTAGGACCAAAGTCTGGCAAAGAGGGACACCTGGCTCTTCTCCATA
  TGCATCTCCGAGACAGTCTCCCTGGTCATCTCCCAAAGTCTCCAAGAAAGTCCACACTGGGAGGAGCAGA
  CCTCCATCTCCTGTGGCCCCGCCTCCACCCCCTCCCCCTCCTCCTCTTCCTCCCCACATGCTGCCTCCTC
  CCCCTCCTCCCCCTGCTCCTCCACTCCCAGAGAAAAAGCTCATCACCAGAAACATTGCAGAAGTCATTAA
  ACAACAGGAAAGTGCCCAGCGCGCCCTACAAAATGGACAGCGAAAGAAAAAAGGGAAAAAGGTTAAGAAA
  CAACCAAACAATATCCTAAAGGAAATTAAGAATTCTCTGAGGTCAGTGCAAGAGAAGAAAATGGAAGACA
  GCTCCCGACCCTCTACCCCACAGAGATCAGTTCATGAGAACCTCATGGAAGCCATTCGGGGGAGCAGTAT
  AAGACAGCTACGGAGGGTGGAAGTTCCCGAAGCTCTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216300 representing NM_053098
  Red=Cloning site Green=Tags(s)
  
  MSTFGYRRGLSKYESIDEDELLASLSPEELKELERELEDIEPDRNLPVGLRQKSLTEKTPTGNFSREALM
  AYWEKESQKLLEKERLGECGKVAEEDKEESEEELIFTESNSEVSEEVCTEDEEESQEEEEDSEEEEDSEE
  EEETTEATKHINGTVSYNSVNTDNSKPKTFKSQIENINLTNGNSGRTQRNSESPAAIHPCGNPTVIEDAL
  EKIRNNDPDTTEVNLNNIENITTQTLSRFAEALKENTVVKTFSLANTHADDAAAIAIADMLKVNEHITSV
  NVESNFITGKGILAIMRALQHNTVLTELRFHNQRHIMGSQVEMEIVKLLKENTTLLRLGYHFELPGPRMS
  MTSILTRNMDKQRQKRMQEQKQQEGHDGGAALRTKVWQRGTPGSSPYASPRQSPWSSPKVSKKVHTGRSR
  PPSPVAPPPPPPPPPLPPHMLPPPPPPPAPPLPEKKLITRNIAEVIKQQESAQRALQNGQRKKKGKKVKK
  QPNNILKEIKNSLRSVQEKKMEDSSRPSTPQRSVHENLMEAIRGSSIRQLRRVEVPEALR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053098
• ORF Size: 1650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053098.2, NP_444328.1
• RefSeq Size: 1967 bp
• RefSeq ORF: 1653 bp
• Locus ID: 93677
• UniProt ID: Q3UHZ5
• Cytogenetics: 6 A3.1
• Gene Summary: Mediates nucleation of actin filaments and thereby promotes actin polymerization (By similarity). Plays a role in the regulation of actin filament length (PubMed:26487682). Required for normal sarcomere organization in the heart, and for normal heart function (PubMed:26487682, PubMed:27274810).[UniProtKB/Swiss-Prot Function]