Poli (NM_011972) Mouse Tagged ORF Clone

CAT#: MG216260

Poli (tGFP-tagged) - Mouse polymerase (DNA directed) iota (Poli) transcript variant 2, (10ug)

Product Images

Circular map for MG216260

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Poli
• Synonyms: Rad30b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216260 representing NM_011972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGAGTCGGAGGAGGAAGGCGGCCCCGCGGAGGAAGAAGACGCTCCTCGTGCCATGGAGCCCT
  TGCACGCGGGGGCGGCGGGCAGCTCGCGGGCAGTTTGCAGTCAAGGGCCACCTACACAAATCTCTTCGTC
  GAGAGTCATAGTCCACGTAGATCTGGATTGCTTTTATGCCCAGGTCGAAATGATCTCCAACCCAGAATTA
  AAGGACAGACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACCTGCAACTATGAAGCCAGGAAACTGG
  GTGTGAGGAAGCTTATGAATGTGAGAGATGCGAAGGAAAAGTGTCCTCAGCTGGTCCTCGTGAACGGAGA
  AGACCTGAGCCGCTACAGAGAGATGTCCTATAAGGTCACAGAATTGTTGGAGGAATTTAGTCCAGCTGTT
  GAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTGGAAAAAAGACTCCAGCAGCTTC
  CAAGTGAGGAGGTTCCGTCAGTGACTGTGTTCGGCCATGTTTACAATAACCAGTCTGTGAACCTACACAA
  CATCATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATGCGGGAAGCCATGTATAATCAG
  CTCGGGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAAAGTTAGTGTCTGGGGTTTTTA
  AACCAAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCATTCACAGTTTGAACCACATAAA
  GGAAATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTGGGAATCAATAGTGTTCATGAT
  CTCCAAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAATTGCTCAGCGTATCCAGCAGC
  TCAGTTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACAGTCCTTTAGTGAAGAAGATAC
  GTTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAATTACTTTCCAGCCTTTTGACC
  AGAGTATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCCGTCGGTACTCTGACAAACACT
  GTAATCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAAGTTAGGGACAGGAAATCATGA
  CTCCATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATGGTGAACGTGAAGATGCCCTTT
  CACCTGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCAGTGCTAAGAAAGGGCCTATGG
  ACTGCTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGCCTTTAAAGTGAAGGACACCCA
  CACGGAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCAAGTAGAAGAATCGAAAGCACA
  GGAACGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAGACACAAGTGACTTGCCCCTCC
  AGGCACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGCAGATATTCAAGAAGAAATCCT
  TTCTGGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGTCCGCTGCATGCCTCTAGAGGA
  GTATTGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCCCCAGAGATACTGCGCTCCCTA
  GCAAGCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACTGAGCCCCGGGAGCACCTCCCA
  TCCATCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAGGATGAGCAAACGAGTCAAGGC
  CCTACTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTTCTGGTTTCCATTCCTTTCCTA
  ATCTGCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCACAAGCAGACAGCCACCGCCTC
  TCACCAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGATTCTGCGGAAGAAAAGCTTCCT
  TTCCCACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGGTCCAAAAGGAACTGATGGCGG
  AATGGGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216260 representing NM_011972
  Red=Cloning site Green=Tags(s)
  
  MGVESEEEGGPAEEEDAPRAMEPLHAGAAGSSRAVCSQGPPTQISSSRVIVHVDLDCFYAQVEMISNPEL
  KDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAV
  ERLGFDENFVDLTEMVEKRLQQLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEMREAMYNQ
  LGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGINSVHD
  LQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVEAKAKIEELLSSLLT
  RVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNMVNVKMPF
  HLTLMSVCFCNLKALSSAKKGPMDCYLTSLSTPAYTDKRAFKVKDTHTEDSHKEKEANWDCLPSRRIEST
  GTGESPLDATCFPKEKDTSDLPLQALPEGVDQEVFKQLPADIQEEILSGKSRENLKGKGSLSCPLHASRG
  VLSFFSTKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLKDEQTSQG
  PTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLESHQGLESRELDSAEEKLP
  FPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011972
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011972.2, NP_036102.2
• RefSeq Size: 2482 bp
• RefSeq ORF: 2214 bp
• Locus ID: 26447
• Cytogenetics: 18 E2
• Gene Summary: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function]