Ikzf4 (NM_011772) Mouse Tagged ORF Clone

CAT#: MG216195

Ikzf4 (tGFP-tagged) - Mouse IKAROS family zinc finger 4 (Ikzf4), (10ug)

Product Images

Circular map for MG216195

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ikzf4
• Synonyms: A630026H08Rik; Eos; Zfpn1a4; Znfn1a4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216195 representing NM_011772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACACACCACCCGCACTCCCTCGCCGTTTCCAAGGCGGCGGCCGCGTTCGCACCCCAGGGTCTCACC
  GGCAAGGGAAGGATAATCTGGAGAGGGAGCTCTCAGGAGGGTGTGCTCCGGATTTCTTGCCTCAGGCCCA
  GGACTCCAACCATTTTATAATGGAATCTTTATTTTGTGAAAGTAGCGGGGACTCATCTCTGGAGAAGGAG
  TTCCTTGGGGCCCCAGTGGGGCCCTCGGTGAGCACCCCAAACAGCCAACACTCTTCACCCAGCCGCTCGC
  TCAGTGCCAACTCCATCAAGGTGGAGATGTACAGCGATGAGGAGTCGAGCAGACTGCTGGGGCCGGATGA
  ACGGCTCCTGGATAAGGATGACAGTGTGATTGTGGAAGACTCATTGTCAGAGCCCTTAGGCTACTGCGAT
  GGAAGTGGGCCAGAGCCTCACTCCCCTGGCGGCATCCGGCTACCCAACGGCAAGCTCAAGTGCGACGTCT
  GCGGCATGGTCTGCATTGGGCCCAATGTGCTCATGGTACACAAGCGCAGCCACACTGGGGAGAGGCCCTT
  CCACTGTAATCAGTGTGGTGCCTCCTTCACACAGAAGGGCAATCTGCTTCGCCACATCAAGCTGCACTCG
  GGGGAGAAGCCCTTCAAGTGCCCCTTCTGCAACTATGCCTGCCGCCGGCGTGACGCACTCACTGGCCACC
  TCCGCACACACTCAGTCTCCTCCCCCACCGTGGGCAAACCCTACAAGTGCAACTACTGTGGCCGGAGCTA
  CAAACAGCAAAGTACCCTGGAGGAGCACAAGGAGAGGTGCCACAACTACCTACAGAGTCTCAGCACTGAT
  GCCCAAGCTCTGACTGGCCAGCCAGGTGATGAAATCCGTGACCTGGAGATGGTGCCTGACTCAATGCTGC
  ACCCATCGACTGAACGGCCAACTTTCATTGATCGTTTGGCCAACAGCCTCACCAAACGCAAGCGTTCCAC
  CCCACAGAAGTTTGTAGGTGAAAAGCAGATGCGCTTCAGCCTCTCAGACCTTCCCTATGATGTGAATGCC
  AGCGGTGGCTATGAAAAGGACGTAGAGTTGGTGGCACACCATGGCCTGGAGCCTGGCTTTGGAGGGTCTC
  TAGCCTTTGTGGGTACAGAGCATCTGCGTCCCCTCCGCCTCCCACCCACCAACTGCATCTCAGAACTCAC
  ACCTGTCATCAGCTCTGTGTACACCCAAATGCAGCCCATCCCCAGCCGACTGGAGCTTCCAGGGTCCCGA
  GAAGCAGGTGAGGGACCGGAGGACCTGGGAGATGGAGGTCCCCTCCTTTATCGGGCCCGAGGCTCTCTGA
  CTGACCCTGGGGCATCCCCCAGCAATGGCTGCCAGGACTCCACAGATACAGAGAGCAACCACGAAGACCG
  GATTGGTGGGGTGGTATCCCTTCCTCAGGGTCCCCCACCCCAACCTCCTCCCACCATAGTGGTGGGCCGG
  CACAGTCCCGCCTATGCCAAAGAGGACCCCAAACCACAGGAGGGGTTACTGCGGGGCACCCCAGGCCCCT
  CCAAGGAAGTGCTTCGGGTGGTGGGTGAGAGTGGTGAGCCAGTGAAGGCCTTTAAGTGTGAACACTGCCG
  CATCCTCTTTCTGGACCACGTCATGTTCACCATCCACATGGGCTGCCACGGCTTCAGAGACCCTTTTGAG
  TGTAACATCTGTGGTTATCACAGCCAGGATCGGTATGAGTTCTCTTCCCACATCGTCCGGGGGGAACATA
  AGGTGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216195 representing NM_011772
  Red=Cloning site Green=Tags(s)
  
  MHTPPALPRRFQGGGRVRTPGSHRQGKDNLERELSGGCAPDFLPQAQDSNHFIMESLFCESSGDSSLEKE
  FLGAPVGPSVSTPNSQHSSPSRSLSANSIKVEMYSDEESSRLLGPDERLLDKDDSVIVEDSLSEPLGYCD
  GSGPEPHSPGGIRLPNGKLKCDVCGMVCIGPNVLMVHKRSHTGERPFHCNQCGASFTQKGNLLRHIKLHS
  GEKPFKCPFCNYACRRRDALTGHLRTHSVSSPTVGKPYKCNYCGRSYKQQSTLEEHKERCHNYLQSLSTD
  AQALTGQPGDEIRDLEMVPDSMLHPSTERPTFIDRLANSLTKRKRSTPQKFVGEKQMRFSLSDLPYDVNA
  SGGYEKDVELVAHHGLEPGFGGSLAFVGTEHLRPLRLPPTNCISELTPVISSVYTQMQPIPSRLELPGSR
  EAGEGPEDLGDGGPLLYRARGSLTDPGASPSNGCQDSTDTESNHEDRIGGVVSLPQGPPPQPPPTIVVGR
  HSPAYAKEDPKPQEGLLRGTPGPSKEVLRVVGESGEPVKAFKCEHCRILFLDHVMFTIHMGCHGFRDPFE
  CNICGYHSQDRYEFSSHIVRGEHKVG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011772
• ORF Size: 1758 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011772.3
• RefSeq Size: 3580 bp
• RefSeq ORF: 1761 bp
• Locus ID: 22781
• UniProt ID: Q8C208
• Cytogenetics: 10 D3
• Gene Summary: DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (PubMed:19696312).[UniProtKB/Swiss-Prot Function]