Nkpd1 (NM_027116) Mouse Tagged ORF Clone

CAT#: MG216193

Nkpd1 (tGFP-tagged) - Mouse NTPase KAP family P-loop domain containing 1 (Nkpd1), (10ug)

Product Images

Circular map for MG216193

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nkpd1
• Synonyms: 2310015G09Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216193 representing NM_027116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGAC
  
  ATGCAGAAGAACTACAATGTGCACTTCACCAAGAACGCCCGGACCCCCAATGAACGATACCTTTTGGATC
  CCGAGTTGGGGCACCAAAAAGGATGCTGCCGTCAGTGGTACCAGGACCCGGTGGCCACTCACACCCATGG
  GCCCTATCAGCTGTCCCCCCAGGCTCACTGGCAGCAGACCTACCATAGCCACCGAGGTGGCAGCGGCTGT
  CGCCGGTGTCCCCAGCCCCTCATTCCGCAGCGACAGCGGCGGCAGCAGCAGCAGCGACAACCCCCACCCC
  TACCTCCCAGCCCTCTGCGGCAGCGGCTCGGTCCAGTTCGCGGGGCCCAGAAGGGGTCACCCGCGATCGC
  CGCTGCCCGCATGGAACCAGCCAGCGCCCCCAAGCCTGTCACCTCCACACCCACCGCAGTATCAGCCACG
  GCCAGCAGCGGCCCAGCCCTACCCTCTGCAGCCGGCGCCCTCCTGGAGCCCAGCGAGCCCACCGAGGCGC
  GGCCACTGCCTGCACCGGCAGCCTGCGGCTCCTTCACCTCCTACGGCGCCGACATCCTGACAGAGGATGA
  TGTCTACTGCAGCTGTCTGGCTAAAACTCTGTGTCACGTGCCCGTCCCTGTGACGGTGGGTTTCTACGCT
  CCCTTCGGCTGCCGTCTTCACTTGATGTTGGATAAAATCATGACGCTGATGCAGCAGGAGGCCGCGCAGC
  GTGAGAGCGAGGAGCTGCAGCGCGTGCAGTGGCAGCCACGTCGCGTGCGCGGCTGGGGCGTCCCGAAGCT
  GCTGTGGTTCCTGGTGTTTCTGCAGCCCGTGATCACGGAGCTGCACCTGCGGCGCAGGAATGTGCGCTTC
  CTGTTCATCCGCTTCAGCGCCTGGCAGTACGCGGGCACCGACAAGCTGTGGGCCGGGCTGGTGACCACGC
  TGTGTGAGGGCATCCGCCACCACTACGGCGCGCTGCCCTTTAGCGTGTACTCGGTGCTAGGCAACAAGCC
  CTGTGGCCCGCGTGATGGCCTCTGCCAACGCGAGTGGCACTGCCGACGTCGCGTGTGCCTGGCGCTGCTG
  GCGCTGCTGGCCGCGCTGTGCCTGGGTGTGGGGCTGCTGTACCTGTCGCTGGGAGGCCACGCGCCGGGCC
  ACGGAGAGCGTGGTGTGCTCAAAGCGCTGGGCGGCGCGGCCACCACGCTATCCGGCTCAGGGCTGCTCAT
  GGCCGTGTACTCCGTGGGCAAGCATCTGTTCGTGAGCCAGCGCAAGAAGATCGAGCGCCTGGTGTCGCGT
  GAGAAGTTTGGCAGCCAGTTGGGCTTCATGTGCGAGGTGAAGAAGGAGGTGGAACTGCTCACGGATTTCC
  TGTGCTTCCTGGAGATCTACCAGCGACGCCGGCTGCGTGTGGTCCTCGAGGTCACTGGGTTGGACACGTG
  CTACCCAGAGCGCGTGGTGGGCGTCCTAAACGCCATCAACACTCTGCTGTCCGACAGCCACGCTCCCTTC
  ATCTTCATCCTGGTGGTGGACCCCAGCATTCTGGCCGCGTGCCTGGAGAGCGCGGGTAACATGAAGGGCA
  CAGCAGACAACGGCTACCTGTTCCTCAACCGCACAGTCACGCTACCCTTCTCCGTGCCGATTATGGGCCG
  TCGCACCAAGTTGCAGTTCCTGCACGATGCGGTGCGTAGCCGGGACGATCTGCTGTTCCGGGAATTGACG
  ATCAAGCTACAGCCGCAGAGCCCCGGGAACTTGGGCGCAGGTGAGGGTACTCAGCTGCTGGCGGTGGAGA
  CGCAGGGGGACGCCGAGCGCACACAGGGCCGCGTGGACGCGGAGGCGGCGCGGCGTATCCAGGAGGCGCT
  GTGCTGCCTGCATGACGAGGGTGATTGCCTGTATGAGTACGTGCCCGACAACGTGGTGTCCATGCGGCGC
  ATTGTCAACACGGTGCCCATCACCGTGCGCCTGTTGCAGCAGCAGCAGCAGCAACAGCCCGACCGCGTGG
  GACCCACACCGCGCCACGCGGTGGCCTGGGTCGTCCTCGCTAACCAGTGGCCTTGTCGCCTCAGCTGGGT
  GCTGCAATGCCTGGAGGACCGGCAGCAGGCAGGGGGCGCGCCAGAGGGGCGCTCGCGTCTCTGGGATGTC
  TTCTGCGACAATAGTCGCGAGCTGCACACCATGACCAAGGCCTTGCAGAACGTACTGGACCTGGATGGCG
  ACCCTGAGCTTTTTGAGCGCTTTCTAGGCACTGATTTCCCCTTCACCGTGGCCGAGGCGCAGAGTTTGCT
  GCGCTGCACAGTCAACCTGGACCATTCCATCCGCCGCCGCATGGGCCTCATCAGGGCCGTCAGCGCGCTC
  AAGCCGCCCAGCCCACCCAAGTCCCCATCCCAGGACGGTCCCCAGGCCAGTCCCAGAGCCATTATCGCCG
  CAGGCACGTCCCATGCGGGCCAAGGATCAGGACACAGTAAAGAGGCACATCAGACCCGGGACCGGACCCA
  TGGGGGCAAGCCAAGGCCGATGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216193 representing NM_027116
  Red=Cloning site Green=Tags(s)
  
  MQKNYNVHFTKNARTPNERYLLDPELGHQKGCCRQWYQDPVATHTHGPYQLSPQAHWQQTYHSHRGGSGC
  RRCPQPLIPQRQRRQQQQRQPPPLPPSPLRQRLGPVRGAQKGSPAIAAARMEPASAPKPVTSTPTAVSAT
  ASSGPALPSAAGALLEPSEPTEARPLPAPAACGSFTSYGADILTEDDVYCSCLAKTLCHVPVPVTVGFYA
  PFGCRLHLMLDKIMTLMQQEAAQRESEELQRVQWQPRRVRGWGVPKLLWFLVFLQPVITELHLRRRNVRF
  LFIRFSAWQYAGTDKLWAGLVTTLCEGIRHHYGALPFSVYSVLGNKPCGPRDGLCQREWHCRRRVCLALL
  ALLAALCLGVGLLYLSLGGHAPGHGERGVLKALGGAATTLSGSGLLMAVYSVGKHLFVSQRKKIERLVSR
  EKFGSQLGFMCEVKKEVELLTDFLCFLEIYQRRRLRVVLEVTGLDTCYPERVVGVLNAINTLLSDSHAPF
  IFILVVDPSILAACLESAGNMKGTADNGYLFLNRTVTLPFSVPIMGRRTKLQFLHDAVRSRDDLLFRELT
  IKLQPQSPGNLGAGEGTQLLAVETQGDAERTQGRVDAEAARRIQEALCCLHDEGDCLYEYVPDNVVSMRR
  IVNTVPITVRLLQQQQQQQPDRVGPTPRHAVAWVVLANQWPCRLSWVLQCLEDRQQAGGAPEGRSRLWDV
  FCDNSRELHTMTKALQNVLDLDGDPELFERFLGTDFPFTVAEAQSLLRCTVNLDHSIRRRMGLIRAVSAL
  KPPSPPKSPSQDGPQASPRAIIAAGTSHAGQGSGHSKEAHQTRDRTHGGKPRPMA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: RsrII-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027116
• ORF Size: 2475 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027116.1, NP_081392.1
• RefSeq Size: 2753 bp
• RefSeq ORF: 2478 bp
• Locus ID: 69547
• UniProt ID: Q0VF94
• Cytogenetics: 7 A3