Phax (NM_001162989) Mouse Tagged ORF Clone

CAT#: MG216138

Phax (tGFP-tagged) - Mouse phosphorylated adaptor for RNA export (Phax) transcript variant 2, (10ug)

Product Images

Circular map for MG216138

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Phax
• Synonyms: 2810055C14Rik; 4933427L19Rik; AU018701; AU018854; D18Ertd65e; p55; Rnuxa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216138 representing NM_001162989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCACTGACCCCCGTTGTAGCCCAGAAAGTCCTAGGTGGGGGCAGCGCTGCGTGCGCACCGGTGT
  CACATTATCGGACTGTTAAACATGTGGACTCCAGCGAGGAGAGTCTAGATTCCGATGACGATTGCTCTCT
  TTGGAAACGCAAGCGACAGAAGTGTCATAATACTCCTCCCAAGCCAGAGCCTTTCCCATTTGGACCAAGT
  GGTCAGAAAACGGCTCTCAACGGAGGAAAGAAGGTGAACAACATCTGGGGCGCGGTGCTCCAGGAACAGA
  ATCAAGATGCGGTGGCCACTGAACTCGGCATCTTGGGAATGGAAGGCTCCATTGACAGAAGCAGGCAGTC
  TGAGACCTATAACTATTTGCTTGCTAAGAAACTTGCTAAGAAGGAATCTCAAGAGTACACAAAGGAATTA
  GACAAAGATCTAGATGAATATATGCATGGTGACAAAAAACCAGGGTCAAAGGAAGACGAGAATGGGCAAG
  GTCACCTCAAGCGGAAACGACCTGTCAGAGACAGACTGGGTAACAGAGTGGAAATGAACTACAAAGGGCG
  CTATGAGATCACAGAAGAGGATGCTCCCGAGAAAGTAGCCGATGAGATCGCCTTCAGGTTGCAGGAACCC
  AAGAAGGACCTGATAGCCCGAGTAGTGAGGATACTTGGGAACAAAAAGGCCATTGAACTTCTGATGGAAA
  CAGCTGAAGTCGAGCAAAATGGTGGTCTTTTCATAATGAATGGTAGCCGAAGAAGAACACCCGGTGGAGT
  CTTTCTGAATCTCCTGAAGAACACACCCAGCATCAGCGAGGAACAGATTAAGGACATTTTCTACGTTGAA
  AATCAAAAGGAATATGAAAATAAAAAAGCTGCTAGAAAAAGAAGAACACAGCTTTTGGGGAAGAAAATGA
  AACAAGCTATTAAAAGTCTGAACTTCCAGGAGGACGATGACACATCTCGAGAAACGTTTGCAAGTGACAC
  TAATGAGGCCCTGGCCTCTCTCGACGAAGCCCAGGAAGGACCTGGCGAGACCAAGCTGGATGCTGAGGAG
  GCCATTGAGGTGGACCACCCTCAGGACTTGGACATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216138 representing NM_001162989
  Red=Cloning site Green=Tags(s)
  
  MTSLTPVVAQKVLGGGSAACAPVSHYRTVKHVDSSEESLDSDDDCSLWKRKRQKCHNTPPKPEPFPFGPS
  GQKTALNGGKKVNNIWGAVLQEQNQDAVATELGILGMEGSIDRSRQSETYNYLLAKKLAKKESQEYTKEL
  DKDLDEYMHGDKKPGSKEDENGQGHLKRKRPVRDRLGNRVEMNYKGRYEITEEDAPEKVADEIAFRLQEP
  KKDLIARVVRILGNKKAIELLMETAEVEQNGGLFIMNGSRRRTPGGVFLNLLKNTPSISEEQIKDIFYVE
  NQKEYENKKAARKRRTQLLGKKMKQAIKSLNFQEDDDTSRETFASDTNEALASLDEAQEGPGETKLDAEE
  AIEVDHPQDLDIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162989
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162989.1, NP_001156461.1
• RefSeq Size: 1783 bp
• RefSeq ORF: 1092 bp
• Locus ID: 56698
• UniProt ID: Q9JJT9
• Cytogenetics: 18 30.63 cM
• Gene Summary: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA (By similarity).[UniProtKB/Swiss-Prot Function]