Trex2 (NM_011907) Mouse Tagged ORF Clone

CAT#: MG216097

Trex2 (tGFP-tagged) - Mouse three prime repair exonuclease 2 (Trex2), (10ug)

Product Images

Circular map for MG216097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trex2
• Synonyms: MGC129476; MGC129477
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216097 representing NM_011907
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCCACCCCGGGCTGAGACCTTTGTATTCCTGGACCTAGAAGCCACTGGGCTCCCAAACATGG
  ATCCTGAGATTGCAGAGATATCCCTTTTTGCTGTTCACCGCTCTTCCCTGGAGAACCCAGAACGGGATGA
  TTCTGGTTCCTTGGTGCTGCCCCGTGTTCTGGACAAGCTCACACTGTGCATGTGCCCGGAGCGCCCCTTT
  ACTGCCAAGGCCAGTGAGATTACTGGTTTGAGCAGCGAAAGCCTGATGCACTGCGGGAAGGCTGGTTTCA
  ATGGCGCTGTGGTAAGGACACTGCAGGGCTTCCTAAGCCGCCAGGAGGGCCCCATCTGCCTTGTGGCCCA
  CAATGGCTTCGATTATGACTTCCCACTGCTGTGCACGGAGCTACAACGTCTGGGTGCCCATCTGCCCCAA
  GACACTGTCTGCCTGGACACACTGCCTGCATTGCGGGGCCTGGACCGTGCTCACAGCCACGGCACCAGGG
  CTCAAGGCCGCAAAAGCTACAGCCTGGCCAGTCTCTTCCACCGCTACTTCCAGGCTGAACCCAGTGCTGC
  CCATTCAGCAGAAGGTGATGTGCACACCCTGCTTCTGATCTTCCTGCATCGTGCTCCTGAGCTGCTCGCC
  TGGGCAGATGAGCAGGCCCGCAGCTGGGCTCATATTGAGCCCATGTACGTGCCACCTGATGGTCCAAGCC
  TCGAAGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216097 representing NM_011907
  Red=Cloning site Green=Tags(s)
  
  MSEPPRAETFVFLDLEATGLPNMDPEIAEISLFAVHRSSLENPERDDSGSLVLPRVLDKLTLCMCPERPF
  TAKASEITGLSSESLMHCGKAGFNGAVVRTLQGFLSRQEGPICLVAHNGFDYDFPLLCTELQRLGAHLPQ
  DTVCLDTLPALRGLDRAHSHGTRAQGRKSYSLASLFHRYFQAEPSAAHSAEGDVHTLLLIFLHRAPELLA
  WADEQARSWAHIEPMYVPPDGPSLEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011907
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011907.4, NP_036037.1
• RefSeq Size: 1034 bp
• RefSeq ORF: 711 bp
• Locus ID: 24102
• UniProt ID: Q9R1A9
• Cytogenetics: X A7.3
• Gene Summary: Exonuclease with a preference for double-stranded DNA with mismatched 3' termini. May play a role in DNA repair.[UniProtKB/Swiss-Prot Function]