Asxl1 (NM_001039939) Mouse Tagged ORF Clone

CAT#: MG215850

Asxl1 (tGFP-tagged) - Mouse additional sex combs like 1 (Drosophila) (Asxl1), (10ug)

Product Images

Circular map for MG215850

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Asxl1
• Synonyms: mKIAA0978
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215850 representing NM_001039939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACAAACAGAAGAGGAAGAAGGAGCGCACGTGGGCCGAGGCCGCGCGCCTGGTGTTAGAAAACT
  ACTCAGATGCTCCAATGACACCAAAACAGATTCTGCAGGTCATAGAGGCAGAAGGACTGAAGGAAATGAG
  AAGTGGGACATCCCCTCTTGCGTGCCTCAATGCCATGCTACATTCCAACTCAAGAGGAGGAGAAGGGCTG
  TTTTATAAATTACCTGGCCGCATTAGTCTTTTCACACTCAAGAAAGATGCAGTGCAGTGGTCTAGAAATG
  CAGCTACAGTGGATGGAGACGAGCCAGAGGACTCCGCTGATGTGGAAAGCTGTGGGTCTAATGAAGCCAG
  CACTGTGAGTGGTGAAAATGATGTATCTCTGGATGAAACATCTTCAAATGCATCCTGCTCTACAGAGTCT
  CAGAGCCGACCCCTCTCCAATCCCAGGGACAGCCACAGGGCTTCCTCACAGGCAAACAAACAGAAGAAAA
  GGACTGGGGTTATGCTACCTCGTGTTGTCCTGACTCCTCTGAAGGTAAACGGGGCCCACGTGGAACCTGC
  GTCAGGATTCTCAGGCCGCCACGCAGATGGCGAGAGTGGCAGTCCATCGAGCAGCAGCAGCGGTTCTCTG
  GCCTTGGGCAACAGTGCCATTCGAGGCCAGGCCGAGGTCACTCGGGACCCTGCCCCCCTCTTAAGAGGCT
  TCCGGAAGCCAGCCACAGGGCAAATGAAGCGCAACAGAGGGGAAGAGGTAGATTTTGAGACGCCTGGGTC
  CATTCTTGTTAACACCAACCTCCGTGCTCTGATAAACTCTCGGACCTTCCATGCCCTGCCACTACACTTC
  CAGCAGCAACTCCTCCTCCTCCTGCCTGAAGTGGACAGACAGGTGGGGACAGATGGCCTGCTGCGCCTCA
  GCGGCAGTGCACTCAATAATGAGTTTTTCACCCATGCAGCTCAGAGCTGGCGAGAACGCCTTGCTGATGG
  TGAATTCACTCATGAGATGCAAGTCAGGCTAAGACAGGAAATGGAAAAGGAGAAGAAGGTGGAACAATGG
  AAGGAAAAGTTCTTTGAAGATTACTACGGACAGAAATTGGGTTTGACCAAAGAAGAATCACTGCAGCAGA
  AAGAGGTCCAGGAGGAGGCCAAAGTCAAGAGTGGTTTATGTGTCTCTGGAGAGTCTGTGCGGCCGCAGCG
  TGGGCCCAACACCCGTCAACGGGACGGACATTTTAAGAAACGTTCTCGGCCAGATCTCCGAACCAGATCC
  AGAAGGAATATATACAAAAAACAGGAGCCAGAACAAGCAGGGGTTGCTAAAGATGCAAGTGCTGCACCAG
  ACGTCTCACTCTCTAAAGATACTAAAACCGACTTAGCAGGGGTGAACAGTACCCCTGGGCCAGATGTGTC
  CTCAGCAACATCTGGACAGGAGGGTCCCAAGTGTCCCAGTGAACCTGTGGCTTCCCAGATCCAAGCAGAA
  AGGGACAACTTGGCATGTGCCTCTGCATCTCCAGACAGAATCCCTACCTTACCTCAGGACACTGTGGATC
  AAGAGACAAAGGATCAGAAGAGAAAATCCTTTGAGCAGGAAGCCTCTGCATCCTTTCCCGAAAAGAAACC
  CCGGCTTGAAGATCGTCAGTCCTTTCGTAACACAATTGAAAGTGTTCACACCGAAAAGCCACAGCCCACT
  AAAGAGGAGCCCAAAGTCCCGCCCATCCGGATTCAACTTTCACGTATCAAACCACCCTGGGTGGCTAAAG
  GTCGGCCCACTTACCAGATATGCCCCCGGATCGTCCCCATCACGGAGTCCTCCTGCCGGGGTTGGACTGG
  TGCCAGGACCCTCGCAGACATTAAAGCCCGTGCTTTGCAGGCCCGAGGGGCGAGAGGTTACCACTGCAAT
  CGAGAGACGGCCACCACTGCCATCGGAGGGGGGGGTGGCCCGGGTGGAGGTGGCAGTGGGGCCATCGATG
  AGGGAGGTGGCAGAGACAGCAGCAGTGGTGATGGTAGTGAGGCCTGTGGCCACCCTGAGCCCAGGGGAGC
  CCCAAGCACCTCTGGAGAGAGTGCGTCAGATCTACAGCGAACACAACTACTGCCGCCTTGTCCTCTGAAT
  GGAGAGCACACTCCAGCTGAAGCTGCCATGCCCAGAGCCAGAAGAGAAGACTCAGCTTCTCTCAGAAAGG
  AAGAGAGCTGCCTGTTGAAGAGGGTCCCAGGTGTGCTTACAAGTGGGCTGGAAGATGCCTCTCAACCCCC
  TATTGCTCCCACTGGAGACCAGCCGTGTCAGGCTTTGCCCCCTCTGTCCTCCCAAACTCCAGTGGCCGAG
  ATGTTAACAGAGCAGCCTAAGTTGCTTCTAGATGATAGAACTGAGTGTGAATCTAGTAGAGAAGATCAAG
  GACCCACCATTCCCTCAGAGAGTAGTTCTGGACGGTTTCCATTGGGAGATCTATTAGGAGGAGGAAGTGA
  CCAGGCCTTTGATAATATGAAGGAGCCTGTAAGTATGACACCTACTTTTATATCTGAATTGTCATTAGCT
  AACTACCTACAGGATAGGCCTGATGATGATGGATTAGGGCTTGGTGCCACAGGCCTACTCATAAGGGAAA
  GTAGTAGACAAGAAGCTTTGACTGAGGCTTTTGCATCTGGCAGTCCTACCTCCTGGGTACCCATTCTGTC
  AAATTATGAGGTAATAAAAACATCTGATCCAGAATCCAGAGAAAACATACCATGTCCGGAGCCCCAGGAT
  GAAAAAGAGTGGGAGAGAGCTGTTCCTCTCATTGCAGCAACAGAAAGTGTGCCCCAACCTGAGAGCTGCA
  TTTCACATTGGACACCTCCTCCAGCAGCTGTGGGCAGCACTGGCAGTGACAGTGAGCAAGTGGACCTTGA
  AAGACTGGAAATGAATGGCATCTCTGAAGCACCAAGTCCTCACAGTGAATCCACAGATACAGCCTCTGAC
  TCCGAAGGCCATCTCTCTGAGGACAGCAGTGAGGTTGATGCAAGTGAAGTCACAGTGGTAAAAGGGTCAT
  TAGGTGGGGATGAAAAGCAAGACTGGGACCCATCTGCCTCACTGTCCAAGGTGAACAATGACCTAAGTGT
  GCTTACAAGGACAGGAGGGGTGGCTGCTTCTCAGAGCTGGGTGTCTAGAGTATGTTCAGTCCCACACAAG
  ATCCCAGACTCTCTGTTGCTGTCCAGTACTGAGTGCCAGCCGAGGTCTGTGTGCCCACTGAGGCCTGGCT
  CTTCAGTGGAGGTTACCAACCCACTTGTGATGCACCTGCTGCATGGTAATTTGCCCTTGGAGAAGGTTCT
  TCCTCCAGGTCACAGAAGCAGCCGACTAGAGTCATCACAGCTGCCACTTAGAGAACAGAGCCAGGATAGA
  GGCACTCTACAAGGTACAGGGGAAAACAATCGCCTAGCTGCCAGAATCAACCCTGGTTCTGCACAAACAT
  TGAAAGAGTCTATTCTGGCCCAGAGCTATGGAGCAAGTGCTGGTCTTGTCAGGGCAATGGCCTCCAAGGC
  TCCTGCAATGTCCCAGAAGATTGCGAAGATGGTTACAAGTTTAGACTCACAGCATCCAGAGACAGAACTG
  ACACCTTCCTCTGGCAATCTGGAAGAAATAGATTCCAAAGAGCATCTCTCTTCCTTCCTTTGTGAAGAGC
  AGAAAGAAGGCCATTCCCTGTCTCAAGGCAGTGATCCAGGTGCGGCCCCAGGCCAATGTCTAGGAGATCA
  CACTACCTCCAAAGTGCCATGTTTCTCCTCCACAAATGTGAGCCTCTCCTTTGGATCTGAGCAGACAGAT
  GGGACCCTGAGTGATCAGAACAATGCTGGTGGTCATGAAAAGAAACTATTTGGTCCCGGGAATACAGTTA
  CCACCCTTCAGTGCCCCAGGTCTGAAGAGCAGACACCACTACCTGCTGAGGTCCCTCCAGTGTTTCCCAG
  TAGGAAGATAGAACCAAGCAAAAACTCTGTGTCTGGTGGTGTGCAAACTACAAGGGAAAACAGGATGCCC
  AAACCACCTCCTGTCTCTGCTGACAGCATCAAGACAGAGCAGACATTTTTGAGGGATCCTATTAAGGCAG
  ATGCAGAGAACAGAAAAGCTGCAGGGTACAGTTCTCTGGAACTAGTGGGTCACTTGCAAGGGATGCCTTT
  TGTTGTGGATCTGCCTTTCTGGAAGTTACCCAGAGAGCCAGGGAAAGGGTTCAGTCAACCCCTGGAGCCT
  TCTTCCATCCCTTCCCAACTCAACATCAAGCAGGCCTTGTATGGGAAGTTGTCTAAACTTCAGCTCAGTC
  CCACCAGCTTTAATTACTCCTCTAGCTCTGCTACCTTTCCCAAAGGCCTTGCTGGTGGTGTGGTGCAGCT
  GAGCCACAAAGCCAGCTTTGGTACAGGCCACACTGCATCACTGTCCTTACAAATGTTCGCTGACAGCAGT
  GCAGTAGAAAGCATCTCTCTCCAATGTGCATGCAGCCTGAAAGCCATGATCATGTGCCAAGGCTGCGGAG
  CATTCTGCCATGATGACTGCATTGGACCTTCAAAGCTCTGTGTATTGTGCCTTGTGGTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215850 representing NM_001039939
  Red=Cloning site Green=Tags(s)
  
  MKDKQKRKKERTWAEAARLVLENYSDAPMTPKQILQVIEAEGLKEMRSGTSPLACLNAMLHSNSRGGEGL
  FYKLPGRISLFTLKKDAVQWSRNAATVDGDEPEDSADVESCGSNEASTVSGENDVSLDETSSNASCSTES
  QSRPLSNPRDSHRASSQANKQKKRTGVMLPRVVLTPLKVNGAHVEPASGFSGRHADGESGSPSSSSSGSL
  ALGNSAIRGQAEVTRDPAPLLRGFRKPATGQMKRNRGEEVDFETPGSILVNTNLRALINSRTFHALPLHF
  QQQLLLLLPEVDRQVGTDGLLRLSGSALNNEFFTHAAQSWRERLADGEFTHEMQVRLRQEMEKEKKVEQW
  KEKFFEDYYGQKLGLTKEESLQQKEVQEEAKVKSGLCVSGESVRPQRGPNTRQRDGHFKKRSRPDLRTRS
  RRNIYKKQEPEQAGVAKDASAAPDVSLSKDTKTDLAGVNSTPGPDVSSATSGQEGPKCPSEPVASQIQAE
  RDNLACASASPDRIPTLPQDTVDQETKDQKRKSFEQEASASFPEKKPRLEDRQSFRNTIESVHTEKPQPT
  KEEPKVPPIRIQLSRIKPPWVAKGRPTYQICPRIVPITESSCRGWTGARTLADIKARALQARGARGYHCN
  RETATTAIGGGGGPGGGGSGAIDEGGGRDSSSGDGSEACGHPEPRGAPSTSGESASDLQRTQLLPPCPLN
  GEHTPAEAAMPRARREDSASLRKEESCLLKRVPGVLTSGLEDASQPPIAPTGDQPCQALPPLSSQTPVAE
  MLTEQPKLLLDDRTECESSREDQGPTIPSESSSGRFPLGDLLGGGSDQAFDNMKEPVSMTPTFISELSLA
  NYLQDRPDDDGLGLGATGLLIRESSRQEALTEAFASGSPTSWVPILSNYEVIKTSDPESRENIPCPEPQD
  EKEWERAVPLIAATESVPQPESCISHWTPPPAAVGSTGSDSEQVDLERLEMNGISEAPSPHSESTDTASD
  SEGHLSEDSSEVDASEVTVVKGSLGGDEKQDWDPSASLSKVNNDLSVLTRTGGVAASQSWVSRVCSVPHK
  IPDSLLLSSTECQPRSVCPLRPGSSVEVTNPLVMHLLHGNLPLEKVLPPGHRSSRLESSQLPLREQSQDR
  GTLQGTGENNRLAARINPGSAQTLKESILAQSYGASAGLVRAMASKAPAMSQKIAKMVTSLDSQHPETEL
  TPSSGNLEEIDSKEHLSSFLCEEQKEGHSLSQGSDPGAAPGQCLGDHTTSKVPCFSSTNVSLSFGSEQTD
  GTLSDQNNAGGHEKKLFGPGNTVTTLQCPRSEEQTPLPAEVPPVFPSRKIEPSKNSVSGGVQTTRENRMP
  KPPPVSADSIKTEQTFLRDPIKADAENRKAAGYSSLELVGHLQGMPFVVDLPFWKLPREPGKGFSQPLEP
  SSIPSQLNIKQALYGKLSKLQLSPTSFNYSSSSATFPKGLAGGVVQLSHKASFGTGHTASLSLQMFADSS
  AVESISLQCACSLKAMIMCQGCGAFCHDDCIGPSKLCVLCLVVR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039939
• ORF Size: 4542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039939.2
• RefSeq Size: 6674 bp
• RefSeq ORF: 4545 bp
• Locus ID: 228790
• UniProt ID: P59598
• Cytogenetics: 2 H1
• Gene Summary: Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG). Acts as a coactivator of RARA and RXRA through association with NCOA1. Acts as a corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1).[UniProtKB/Swiss-Prot Function]