Snx10 (NM_028035) Mouse Tagged ORF Clone

CAT#: MG215662

Snx10 (tGFP-tagged) - Mouse sorting nexin 10 (Snx10) transcript variant 1, (10ug)

Product Images

Circular map for MG215662

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snx10
• Synonyms: 2410004M09Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215662 representing NM_028035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCAGAACAGCAGAAAGAGGAGTTCGTGAGTGTCTGGGTCCGGGACCCCAGGATTCAGAAGGAGG
  ACTTTTGGCACTCTTATATCGACTATGAGATCTGCATTCACACAAATAGCATGTGTTTTACAATGAAAAC
  ATCTTGTGTACGAAGAAGGTATAGAGAGTTCGTGTGGCTGAGGCAAAGACTCCAAAGCAACGCGTTGCTG
  GTACAATTACCAGAACTTCCGTCTAAAAACCTGTTTTTCAACATGAACAATCGCCAGCATGTCGACCAAC
  GCCGCCAGGGCTTGGAAGATTTCCTCAGAAAAGTCCTGCAGAATGCACTCTTGCTTTCCGATAGCAGCCT
  CCACCTCTTCCTGCAGAGCCATCTGAACTCCGAGGACATTGAAGCATGTGTTTCTGGGCAGACCAAGTAC
  TCGGTGGAAGAAGCCATTCACAAGTTTGCTTTAATGAATAGACGGTTTCCCGAAGAAGAAGAAGAAGGGA
  AGAAAGACGCCGACGTAGAGTATGATTCAGAAAGTTCATCCTCTGGGCTTGGACACAGTAGTGATGACAG
  CAGTTCTCATGGATGTAAAACGAGCCCAGCTCTGCAGGAGTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215662 representing NM_028035
  Red=Cloning site Green=Tags(s)
  
  MFPEQQKEEFVSVWVRDPRIQKEDFWHSYIDYEICIHTNSMCFTMKTSCVRRRYREFVWLRQRLQSNALL
  VQLPELPSKNLFFNMNNRQHVDQRRQGLEDFLRKVLQNALLLSDSSLHLFLQSHLNSEDIEACVSGQTKY
  SVEEAIHKFALMNRRFPEEEEEGKKDADVEYDSESSSSGLGHSSDDSSSHGCKTSPALQES
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028035
• ORF Size: 603 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028035.4, NP_082311.3
• RefSeq Size: 2567 bp
• RefSeq ORF: 606 bp
• Locus ID: 71982
• UniProt ID: Q9CWT3
• Cytogenetics: 6 B3
• Gene Summary: Probable phosphoinositide-binding protein involved in protein sorting and membrane trafficking in endosomes. Plays a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium. Required for the localization to the cilium of V-ATPase subunit ATP6V1D and ATP6V0D1, and RAB8A. Involved in osteoclast differentiation and therefore bone resorption.[UniProtKB/Swiss-Prot Function]