Scel (NM_022886) Mouse Tagged ORF Clone

CAT#: MG215358

Scel (tGFP-tagged) - Mouse sciellin (Scel), (10ug)

Product Images

Circular map for MG215358

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Scel
• Synonyms: 9230114I02Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215358 representing NM_022886
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATTTTTCCTCTAGAAAAAAGTCTCCAACTGGAAATGATCTGAAGAGCGCCCGGGGAGCTGAGC
  TGAGACAACAGGGTTTTCAGGACGTGAACAAAAGAAGGGCTTTCTTACAGGACAACAGCTGGATAAAGAA
  GCCCCCAGAGGAAGAACAAGACGGAAATTATGGCAGGGTGGTGCTCAACCGGCACAACTCCCATGATGCC
  TTGGACAGGAAATTAATAGAGAGAGATGAGCCAAAAGCCACAATTAGCCGGTACAGATCTGAGGACATGC
  TGGACAGAACACTGTCCTCATTTAGAACGCCACAATCAACCAAGACGCCAGCTGTCAGCTCCTTCAATGC
  AAACACTACCGCCACAGCTTCAACCCCCGCTACCACTCCTGTGAAGAAAAAGAGACAGTCCTGGTTCCCA
  CCACCCCCTCCTGGTCACAATGCCTCTCCAAGCACAGCAGCCAGCAGAAGGGACCCAGCTCTGCACCCTC
  CACTCCCCCCAAAGCCCTGTTCTCCCATTGCTTCTCCTAAACCATTGGGGCGGACTAATAGGCAGATACA
  TGCAGCTACAGCGGGGGCATGTGGAGAAACAGAACGACATGCTGAAAGAAATATAAGGACTGAGGATCTC
  GATGACATCATCAGGGTGGCGGCTGCACTTCAGAAAACTGACAAGGGTGAAGAATTGGATAATCTCATCA
  GAATGAACAAAAGCTTGAACAGAAACCAAGGTCTCGATGGCCTCTTCCGAGCAAACCTGAAGGCACAGCA
  ATTGGACAAAAGAGCCCAGAGTCTTGAAAGCCTGATCTATATGAACACACAGACGGACAGAGATGGCAAA
  GGAAATCAAGCCTTCGGGAGCCTTAAGAAAATCAACCAAAGGGCTGACCCCGACAGAAGAAGCCAAGATC
  TCAGATCAGTTATCAGAACACATGCCACGGCTGAGAGAATCGGCAGAAGAAAACAAGACCTCGATGGACT
  GATTAAAGTGAATCCTGACACAAATAAAAATATGAAGAGGGGCAAGAGCCTTGACAATCTTATCAAAGTC
  ACCCCGGAAGTAAACAGAAGCAACAAAGGGGGTCCAAGTCTTGACAATTTCACCAAAGGAGTTCCTGCAA
  GGAGCAGAGCTAACCAAAGGGACCAAGACCTGGACAGTCTTATCAAGGTGACCCCCTCAGCAAACAGAAG
  CAGCCAGCACAGTCTTGATGAACTTATTAATACAAGCCCCCAAACTATCAAAACTACTGCTAGACACCAA
  GATCTCGATAAGTTCATCAAAGTAAATCCTGATGTTCTCACAAACAACCAAAGAAACCATGATGTAGACA
  GCACCATCAGAGGGAATCCTACAGGGACCAGATGCGAGCAAAGCGAAGAGTTGGACAATCTTATCAAAGT
  GAAGCCTTCTGCTCTCAGAAACACAAATGGAGGCCAGGACCTAGAAAGCTTAACTGAAGTAAATTCTCAT
  GTGGCTGAGAAGAATGGGAGGATAGATGGCCAAGCCAATGGATTGACTAACAGTCTGTTCAAGGAGTCCA
  CAAGAGCCTCGGTCTATTCTTATGAAGCCAGAAACAGTCTCAGCTCCAATTCTGGAAACAAGAATGGTGG
  ACCCAAGGACACTGTTGTTTATACAAGGACATATGTGGAGAACAGTAAATCACCAAAGGATGGTTATCAG
  GAAAATATCTCAGGAAAATATATACAAACCGTTTATTCAACTTCAGATAGGTCTGTCATTGAAAGAGACA
  TGTGCACTTACTGCCGCAAGCCCTTGGGGGTGGAAACCAAGATGATTTTAGATGAATTACAGATCTGTTG
  CCATTCCACCTGCTTTAAGTGTGAAATATGCAAACGGCCTCTGGAAAATTTACAGGCAGGAGACAGTATT
  TGGATCTATAGGCAGACGATACACTGTGAGCCCTGCTACTCTAAAGTGATGGCAAAATGGATTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215358 representing NM_022886
  Red=Cloning site Green=Tags(s)
  
  MSNFSSRKKSPTGNDLKSARGAELRQQGFQDVNKRRAFLQDNSWIKKPPEEEQDGNYGRVVLNRHNSHDA
  LDRKLIERDEPKATISRYRSEDMLDRTLSSFRTPQSTKTPAVSSFNANTTATASTPATTPVKKKRQSWFP
  PPPPGHNASPSTAASRRDPALHPPLPPKPCSPIASPKPLGRTNRQIHAATAGACGETERHAERNIRTEDL
  DDIIRVAAALQKTDKGEELDNLIRMNKSLNRNQGLDGLFRANLKAQQLDKRAQSLESLIYMNTQTDRDGK
  GNQAFGSLKKINQRADPDRRSQDLRSVIRTHATAERIGRRKQDLDGLIKVNPDTNKNMKRGKSLDNLIKV
  TPEVNRSNKGGPSLDNFTKGVPARSRANQRDQDLDSLIKVTPSANRSSQHSLDELINTSPQTIKTTARHQ
  DLDKFIKVNPDVLTNNQRNHDVDSTIRGNPTGTRCEQSEELDNLIKVKPSALRNTNGGQDLESLTEVNSH
  VAEKNGRIDGQANGLTNSLFKESTRASVYSYEARNSLSSNSGNKNGGPKDTVVYTRTYVENSKSPKDGYQ
  ENISGKYIQTVYSTSDRSVIERDMCTYCRKPLGVETKMILDELQICCHSTCFKCEICKRPLENLQAGDSI
  WIYRQTIHCEPCYSKVMAKWIQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022886
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022886.2, NP_075024.2
• RefSeq Size: 3053 bp
• RefSeq ORF: 1959 bp
• Locus ID: 64929
• UniProt ID: Q9EQG3
• Cytogenetics: 14 E2.3
• Gene Summary: May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope (By similarity).[UniProtKB/Swiss-Prot Function]