Alpi (NM_001081082) Mouse Tagged ORF Clone

CAT#: MG215311

Alpi (tGFP-tagged) - Mouse alkaline phosphatase intestinal (Alpi), (10ug)

Product Images

Circular map for MG215311

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alpi
• Synonyms: 2010001C14Rik; Akp6; gIAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215311 representing NM_001081082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGAGACTGGGTGCTGCTGTTGTTTTTGGGTCTCAGGATCCATCTGTCCTTTGGTATCATCCCAG
  CTGAAGAGGAGAACCCGGCCTTCTGGAACAAGAAGGCAGCTGAAGCCCTGGATGCTGCCAAGAAGCTGCA
  GCCCATTCAGACATCAGCTAAGAACCTCATCATCTTCCTGGGAGACGGGATGGGGGTACCCACAGTGACA
  GCCACCAGGATCCTAAAGGGGCAGTTGGAAGGCCATCTAGGACCGGAGACACCCCTAGCCATGGACCTCT
  TCCCATACATGGCTCTGTCCAAGACATACAACGTGGACAGACAGGTCCCAGACAGCGCAGGCACAGCAAC
  CGCCTATCTCTGTGGGGTCAAGGCCAACTACAAGACCATCGGCCTGAGTGCAGCTGCTCGATTAGACCAG
  TGCAACACCACATTTGGCAATGAGGTCTTCTCAGTGATGTACCGTGCCAAGAAAGCAGGGAAGTCTGTGG
  GAGTGGTGACCACGACCAGGGTGCAGCATGCCTCTCCAGCCGGCACCTACGCACACACGGTGAACCGTAA
  TTGGTACTCAGATGCAGAGATGCCTGCCTCTGCACTGCAGGACGGCTGCAAGGACATCGCCACTCAACTC
  ATCTCCAACATGGACATTGATGTGATCCTCGGTGGAGGCCGAAAGTTCATGTTTCCCAAGGGGACTCCAG
  ACCCTGAGTACCCAAGTGACAGTAACCAGTCTGGAACCAGGCTGGATGATCAGAACCTGGTGCAAACGTG
  GCTGTCAAAGCATCAGGGAGCCCGGTATGTTTGGAACCGATCAGAGCTCATTCAGGCATCCCAGGACCCA
  GCAGTAACTCACCTCATGGGCCTCTTTGAGCCAACAGAAATGAAATATGATGCCAACCGAAACCCCTCAG
  TAGACCCTTCCCTGGCGGAGATGACAGAGGTGGCTGTGCGCATGCTCAGCAGGAATCCACAAGGCTTCTA
  CCTCTTTGTAGAAGGGGGTCGTATTGACCAAGGCCACCATGCGGGCACAGCTTACCTGGCACTGACTGAG
  GCTGTCATGTTCGACTCTGCCATCGAAAAGGCCAGCCAGCTTACCAATGAGAAGGACACGCTAATACTCA
  TCACTGCTGACCACTCCCATGTCTTCGCATTTGGTGGCTACACACTTAGGGGGACCTCCATCTTTGGTCT
  GGCCCCACTCAAAGCCCTAGATGACAAATCCTACACCTCCATTCTCTATGGCAATGGTCCAGGTTATGAG
  CTGAAATCAGGCAACCGGCCCAATGTCACCGAAGCTCAGAGTGTTGATCCCAACTACAAGCAGCAGGCGG
  CTGTACCCCTGTCGTCAGAGACCCACGGCGGGGAGGACGTGGCCATATTTGCGCGCGGCCCTCAGGCACA
  CCTGGTGCACGGAGTTCAGGAGCAGAACTACATCGCGCACGTCATGGCATTCGCAGGCTGCTTAGAGCCC
  TACACCGACTGCGGCCTGGCGCCCCCTGCCGGCCAGAGCCCCGTCATCACCCCGGGCCAGGCCACCACCA
  CAAACAACGCGGCCGGCCAGGCCACCACCACAAACAACGCCGCCGGCCAGGCCACTGTCCTGCTGTCCTT
  GCAACTGCTGGTCAGCATGCTGCTGCTAGTAGGGACAGCCATGGTGGTGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215311 representing NM_001081082
  Red=Cloning site Green=Tags(s)
  
  MQGDWVLLLFLGLRIHLSFGIIPAEEENPAFWNKKAAEALDAAKKLQPIQTSAKNLIIFLGDGMGVPTVT
  ATRILKGQLEGHLGPETPLAMDLFPYMALSKTYNVDRQVPDSAGTATAYLCGVKANYKTIGLSAAARLDQ
  CNTTFGNEVFSVMYRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDAEMPASALQDGCKDIATQL
  ISNMDIDVILGGGRKFMFPKGTPDPEYPSDSNQSGTRLDDQNLVQTWLSKHQGARYVWNRSELIQASQDP
  AVTHLMGLFEPTEMKYDANRNPSVDPSLAEMTEVAVRMLSRNPQGFYLFVEGGRIDQGHHAGTAYLALTE
  AVMFDSAIEKASQLTNEKDTLILITADHSHVFAFGGYTLRGTSIFGLAPLKALDDKSYTSILYGNGPGYE
  LKSGNRPNVTEAQSVDPNYKQQAAVPLSSETHGGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEP
  YTDCGLAPPAGQSPVITPGQATTTNNAAGQATTTNNAAGQATVLLSLQLLVSMLLLVGTAMVVS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081082
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081082.2, NP_001074551.1
• RefSeq Size: 2335 bp
• RefSeq ORF: 1665 bp
• Locus ID: 76768
• Cytogenetics: 1 C5