T2 (NM_001161832) Mouse Tagged ORF Clone

CAT#: MG215272

T2 (tGFP-tagged) - Mouse brachyury 2 (T2), (10ug)

Product Images

Circular map for MG215272

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: T2
• Synonyms: brachyury2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215272 representing NM_001161832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGATCTTGAGAGACCATTTTAAAACGCCAAAGAAACACAGGGGGTGTCACACCCAAGTGGAGG
  ACAAAGAGAAGGCAGCACAGCAGACCTTGCCGGAACAGTCGCAGCCCGAAGCTTGGTCAGCATCCCCGCC
  CAAGGCTTCAACCAGGTTCCCTTCCAGGTCCAACGCCTACCTGATTATAGGGTTGCAAAACGAGCTGACC
  AAATGTGGGATCTTGAAGAACATGAGCGACGACGAGGATTTCTGGAAACTGGTTCAGGAGGAAGCTCTTG
  GAGTGGGAACTAAAGAAAAGCTCCAGAAGATAAAACTTAAGTTGATGAATCCCAGGCCCTGGCCACCAGC
  TGCTTCCAGGAGAAGGGAGACCGAGATGCTGGTCAGCAGTGATCAAGACAGTGGGGCACGCCAGGGAACA
  CAGGACCATCCAGGGAGAGAACAGGAAGTTGTGGAGGATGGCCCGGAGAAGGAGAGGATAAAACAGGGCC
  CGCACCAACCTGGCTTGGATCCACCCAAGCATCGAGGGGACACAGCGAGTCTTCTCGCTCGGCTCACGGA
  ATGGAGAAAAAGATGCCAACAGCATGGAGCTGCCCAGAGGCTGCATCGAGGACCTGAGGAGAGCAAGACT
  ATGTGTAAGACAAGGACGGATTCCCAAAGCAAGATGTATCTTAGCCGGTTGTATCGGATGTATTCCACCT
  CCCTTGCTAACATGGAATTCTCCAGAAAACTGCTGGAAAGAGATGGCCGCTTTGCAGACATGGATGCTGA
  GCACAGGGCCCGAAGCCTGCTGGATTACATGGTCCCAAGAGAACGTACACAGGCAGATGTTCCACCAAGA
  GAACGTACACAGGCAGATGTTCCACCAAGGGAACTAGAGGAGGAGGACTCCCCAACCCCTGAGCAGCAGC
  CTCAAGCAGGACCTGCCCTCCGATTTCTGAAGTGCCAGAGCCCAGAAAGTCCACAGAGACGTCCCCACCT
  GAAGACAGGAAGACACCAAGTGACCCTGTGTGGCACGAGCAAGCTCTCCGAACACGAGAGGGGCACACTT
  GCTCCTGCCAGGAGGACCACAGATCCCAGACCTACAGCACCCCTGACCCTTGCACACGTGATCCAGACCC
  ATCCTGTGGTGGAGGCCAAGACAGCAAGTCGATACTGGGTGAACAATGTGGATGAAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215272 representing NM_001161832
  Red=Cloning site Green=Tags(s)
  
  MAQILRDHFKTPKKHRGCHTQVEDKEKAAQQTLPEQSQPEAWSASPPKASTRFPSRSNAYLIIGLQNELT
  KCGILKNMSDDEDFWKLVQEEALGVGTKEKLQKIKLKLMNPRPWPPAASRRRETEMLVSSDQDSGARQGT
  QDHPGREQEVVEDGPEKERIKQGPHQPGLDPPKHRGDTASLLARLTEWRKRCQQHGAAQRLHRGPEESKT
  MCKTRTDSQSKMYLSRLYRMYSTSLANMEFSRKLLERDGRFADMDAEHRARSLLDYMVPRERTQADVPPR
  ERTQADVPPRELEEEDSPTPEQQPQAGPALRFLKCQSPESPQRRPHLKTGRHQVTLCGTSKLSEHERGTL
  APARRTTDPRPTAPLTLAHVIQTHPVVEAKTASRYWVNNVDEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161832
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161832.1, NP_001155304.1
• RefSeq Size: 1755 bp
• RefSeq ORF: 1182 bp
• Locus ID: 21331
• Cytogenetics: 17 4.92 cM