Tcp11x2 (NM_027952) Mouse Tagged ORF Clone

CAT#: MG214962

1700008I05Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700008I05 gene (1700008I05Rik), (10ug)

Product Images

Circular map for MG214962

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tcp11x2
• Synonyms: 1700008I05Rik; C77164; TCP11; Tcp11l3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214962 representing NM_027952
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAGAGTGAGAAAATTATATCCAAAAAGGACTCCAGTCCATCTGAAAATGGATCCCCAAAACCAG
  AAACATCAAGCAAGAACCAGGAAGATAAGACCAGTTCAGAGGTCCACTCTGCAGAGAACCAGCTTGCAGC
  AATTTCTAATGAAGATACAGGAAAAGTTAATGAAGTTTCCAAATTGAATGTTGCTTCTGAAATTGCCATA
  GAAAAGAATGATTTACCTGAAGACAGTCCAGAAAGCCAATTCGTGGAGACAATGTACAATGCATTTTGGG
  ACCACTTGAGAGATCAACTGTCACAAACCCCTCCTGACTTTTCTTGTGTTCTTGAACTTATAAAAAACAT
  AAAACAGATTTTAATGGCACTACTATTACCACGTCAGAATCTTCTAAGAAATGAGATTGAAGAGGTACTG
  GATTTGGATCTCCTCAAACAAGAAACAGAGCACGGGGCTCTGGATATTCTGCATCTCTCCCACTATATTC
  TCAACTTGATGGCCCTCATGTGCGCACCAATTAGAGATGAAGCTGTAAAGGAATTAGAAAACATAAAAGA
  TCCAGTACAGTTACTGAGGGGTATCTTCCAAGTTTTAGGCCTGATGAAAATGGATATGGTGAACTATACT
  ATTAAGAACATTCGACCATATTTACAGGAACATTCTATACAATATGAACGAGCTAAATTTCAAGAACTCC
  TTGACAAACAGCCTGATCGACTTGATTGCACCACAAAATGGATGACTAGAGCTGCCACAGAGATTATTAC
  ATCACTTTCAAGTTTATCTGTCACTTCATGTATCTCTTCTGGCGTAATAAGTTCATTTCCTACTCCAAAG
  CCTGACCCAGATCCACCCAGTCTTACAACAGTGCTATACCAAGGGTACCTAAATCTTGTTCTCAGGGACC
  ATGGGAATGAAGAATTCCCTGAGACTCTGCTGATGGATAAAGCACGACTGCAGACTATGAGATCCAAATT
  TCGCCACTTGACTATTCTAGCCTCAGTTTTACTGGTGGCAAGAAGTTTCTCTTGTGGTGTTTTATTCAGC
  TCACCAGAATTTGTGGATAAATTGAAGTGCATAACAAAGGCTCTAACTGAAGATTTTAGCTCCAAGCCAG
  AAGAATCTATGTTAAGTGTGAGTGAACAAGTGTCTCAGGAAATCCATCAAGGTCTCAAAGACACAGGCCT
  AACTGCTCTGAGCAGTGAAAACAGGGCATCACTTGTAGGACAACTGCGGAACATTGCCAAGAAAGAGAAT
  TGCATTCGTATCATCATTGAACAACGGATGCATTTATTTCTTAAATGCTGTTTGATTCGTGGCATGCAAG
  AATCTCTTCTAGACTTCCCTGGATGCTTTCTTTTCATTGAGCCAGAGTTAGCAGAACTAGGATGGAAGTT
  TGTTAATCTGATGCACCACAACCAGCATGTGTTTAGCCCGTACTATGCTGAAATCCTAAAAAACATCATC
  TCAACACAAAATGATGAAGACGAAGAAAAGCCAATCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214962 representing NM_027952
  Red=Cloning site Green=Tags(s)
  
  MPKSEKIISKKDSSPSENGSPKPETSSKNQEDKTSSEVHSAENQLAAISNEDTGKVNEVSKLNVASEIAI
  EKNDLPEDSPESQFVETMYNAFWDHLRDQLSQTPPDFSCVLELIKNIKQILMALLLPRQNLLRNEIEEVL
  DLDLLKQETEHGALDILHLSHYILNLMALMCAPIRDEAVKELENIKDPVQLLRGIFQVLGLMKMDMVNYT
  IKNIRPYLQEHSIQYERAKFQELLDKQPDRLDCTTKWMTRAATEIITSLSSLSVTSCISSGVISSFPTPK
  PDPDPPSLTTVLYQGYLNLVLRDHGNEEFPETLLMDKARLQTMRSKFRHLTILASVLLVARSFSCGVLFS
  SPEFVDKLKCITKALTEDFSSKPEESMLSVSEQVSQEIHQGLKDTGLTALSSENRASLVGQLRNIAKKEN
  CIRIIIEQRMHLFLKCCLIRGMQESLLDFPGCFLFIEPELAELGWKFVNLMHHNQHVFSPYYAEILKNII
  STQNDEDEEKPIK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027952
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027952.3, NP_082228.2
• RefSeq Size: 1776 bp
• RefSeq ORF: 1512 bp
• Locus ID: 71841
• Cytogenetics: X F1