Gm4305 (NM_001166639) Mouse Tagged ORF Clone

CAT#: MG214815

Gm4305 (tGFP-tagged) - Mouse predicted gene 4305 (Gm4305), (10ug). Note: ORF is codon optimized

Product Images

Circular map for MG214815

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gm4305
• Synonyms: 100043235
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214815 representing NM_001166639, codon optimized.
  Due to the complexity of NM_001166639, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACTATGGCTGATAAGATGGACATGTCTCTCGAGGACATTATTAAGCTGAACAAGATGCAGCAGG
  GCCGCAGGGACCGGCCAGATAGCAGGGTGAAACGCGGTACTGGCCCTAAAAGGTATAGGCCTGCTTTCAC
  ACATGGCGGTCGAAATAGACTGGCACCTTATTGCAGACCAAAACAGCTGCCTGACAAGTGGCAACACGAC
  CTGTTTATCGGTGGTTTTAGGGGCCAGAACCACGTGGATACCGGCGGAAAGCTCTTTCTTTCCAACCTGC
  ACTTCGGTGTATCCGATGCTGACATACAGTTGCTGTTCGCCGAGTTCGGTACTCTGAAGAAAAGTGCCGT
  CCACTATGACAGATGTGGTCGGAGTCTGGGCACCGCCCACGTCCACTTCGAGCGAAAGGCTGACGCCCTG
  AAGGCAATGAGGGAATACAATGGGGCGCCACTGGATGGCCGCCCTATGAACATACACCTGGTGACCAGCC
  AGATCGACCGCCAAGGGCGCCCCGCCCTTAATTCAGATAAAGGCGGTATGACCAGGAACCCTGGGTCTGG
  GGTGCTGAGTGGAGGAGGGACTAAGCGATGGACACTGGGGGGGAGCCAGGGCTCCGGCAGAGGGACTTCC
  CGCAACAGCAAGTTGCAGCAGCAACAGCAGCAGCAACAGCAGCAGGAAGAACAGAAACACCAGAAGCAGC
  AGCAGCAGAAGCAGCAACAACAGCAGCAGAAACAGGGGCAGAATCATCAACACCAGCAACAACAGAAAGA
  GCAGCAACAGCAGCAGCAGCAGAAAGAGCTTCAGCAGTTGAGCGTCGAAGAGCTGGATGCTCAACTCGAT
  TACTACCAGGATATGATGGACACAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214815 representing NM_001166639
  Red=Cloning site Green=Tags(s)
  
  MTTMADKMDMSLEDIIKLNKMQQGRRDRPDSRVKRGTGPKRYRPAFTHGGRNRLAPYCRPKQLPDKWQHD
  LFIGGFRGQNHVDTGGKLFLSNLHFGVSDADIQLLFAEFGTLKKSAVHYDRCGRSLGTAHVHFERKADAL
  KAMREYNGAPLDGRPMNIHLVTSQIDRQGRPALNSDKGGMTRNPGSGVLSGGGTKRWTLGGSQGSGRGTS
  RNSKLQQQQQQQQQQEEQKHQKQQQQKQQQQQQKQGQNHQHQQQQKEQQQQQQQKELQQLSVEELDAQLD
  YYQDMMDTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166639
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166639.1, NP_001160111.1
• RefSeq Size: 910 bp
• RefSeq ORF: 870 bp
• Locus ID: 100043235
• Cytogenetics: 10