Dpy19l2 (NM_001166207) Mouse Tagged ORF Clone

CAT#: MG214684

Dpy19l2 (tGFP-tagged) - Mouse dpy-19-like 2 (C. elegans) (Dpy19l2), (10ug)

Product Images

Circular map for MG214684

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dpy19l2
• Synonyms: 4932443J21Rik; Gm18376
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214684 representing NM_001166207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGCCGACAAGGAGTAAGCTCAGGGAAGGGAGCTCCGACAGGCCACAATCCTCCTGCACGGGGC
  AGGCGAGGAGGAGGTGGAGCGCAGCGACCATGGAGCCCCAGCAGGAGCGAAGCGCGCCCCAGGAGAGAAC
  GAAGTGGTCGTTGCTGCAGCATTTCCTCCTAGGCGGCAGGAAACTGCCCTCGGGTGCTCGCAACTACGCG
  GCGCGGAGGATCCAAAGTCTGAACGCACAAAATTACTTTCAGCTGGAAGAGGTCGCCAAGCTCCTTCTTC
  TTAACCGCTTCCAATTCCTCTTCACTCTCCTGGACCACTTTCGCGAAAAGGTGCAGGCACTGCAGATGCA
  TCGGTTCTCCCACAGGACCCTCTTTGGCCTAGCTATCTTTGTGGGGATTTTGCACTGGTTACACTTAATA
  ACACTTTTTGAAAATGATCATCATTTTTCTCACCTTTCATCTTTGGAACGGGAGATGACTTTTCGCACAG
  AAATGGGACTTTATTATTCCTACTTCAAGACCATTATCGAAGCACCTTCGTTTCTGGAAGGACTGTGGAT
  GATCATGAATGACAGGCTCACTGAGTATCCCCTGGTAATCAACACAGTGAAACGCTTCCATCTTTACCCA
  GAGGTAGTCATAGCCTACTGGTATCGCACAATCATAGGAATAATGAATTTATTTGGGATAGAAACTAAGA
  CATGTTGGAACGTCACCAGAATGGAGCCTCTTAATGAAGTTCAGAGCTGTGAAGGATTGGGAGACCCAGC
  TTGCTTTTACATCGGAGTAATTTTTATTTTAAATGGACTAATGATGGGCTTGTTCTTCATATACAGTACA
  TACCTGAGTGGGAGTCAGTTAGGAGGTCTGATTACAGTAGCCTGCTACTTTTTCAATCATGGAGAGGCCA
  CCCGTGTGATGTGGACACCGCCTCTCCGGGAAAGTTTCTCCTACCCATTCCTTGTACTTCAGATGTACAT
  CTTAACTATAATCCTCAGGACCTCCACCGTTCACAAAAAACACTACATGGCTCTCTGCTTCTCCAACGTC
  GCTTTTATGCTTCCGTGGCAGTTTGCTCAGTTTATACTTTTTACACAGATAGCCTCATTATTTCCCATGT
  ATGTTGTGGGATACATTGAGCCAAGCAAATTTCAGAAGATCATTTATGTCAATATGAGTTCGGTTGCCCT
  ATGCTTCATTTTGATGTTTGGGAATTCAATGTACTTAAGTTCATATTATTCCTCATGTTTGTTAGTGACA
  TGGGCAATAATGCAAAAGAAAAGCAAAATCCAAAAATTGGGAGGCACTGAACTTCAGTTTTGGCTAATCC
  AAGGCTGTTTTTGGTGGTGTGGAACAATTATTTTGAAATTTCTGACATCTAAAATCTGTGGTGTTTCAGA
  CCATATTCGCCTGAGTGACCTTATAGCAGCCAGAATACTAAGGTATACAGATTTTGATACCTTAATATAC
  ACCTGTGCTCCCGAATTTGACTTCATGGAACAAGCGACTCCGCTGAGATATATAAAAACATTATTGCTTC
  CACTTATCCTAGTGATTACTTATCTTATCTTTAAAAAGATTGTTCGTGATATCATGTGTGTCTTATATAC
  AAACACTTATGTAAGAAAACAGCTTCTTGACAACGCTGAGTTGATTTTCCACACCTTGCAGTTGCTGGCA
  TTCACAGGTCTTGCCATCTTAATCATGCGGTTGAAGCTGTTTCTGACCCCCCACATGTGCATTATGGCTT
  CCTTGATCTGCTCACAACGGCTCTTTGGCTGGCTTTTTTGCAGAATTCATTTTGAGAATGTTGTCTTCGG
  CATTCTGACAATGATGTCAATACAAGGTTGTGCAAATCTCCATAACCAATGGAGCATAATGGGAGAATTT
  ACTAACTTGCCTCAGGAAGAGCTCATACATTGGATCAAACACAGCACCAGGCCAGATGCCGTCTTTGCAG
  GTGCCATGCCTACAATGGCAAGCATCAAGCTGTCTACACTGCGTCCAATTGTGAACCACCCACACTATGA
  GGACGCAGACTTGAGGGCTCGGACAAAAATAGTTTATTCTGTGTATAGTCGAAAATCTGCAGTAGAAGTG
  AGAAATAATCTGTTGAAATTACATGTGAATTATTATGTTCTAGAAGAGGCATGGTGTGTCGTGAGAACAA
  AACCTGGTTGCAGCATGCTTGAAATATGGGATGTGGAAGACCCGTCCAACGCAGCTAACCCTCCCCTCTG
  CAGTATCCTGCTCAAGGATTCTCGGCCGTACTTTACCACAGTGTTTCAGAATAGTATGTACAGAGTACTG
  AAGATTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214684 representing NM_001166207
  Red=Cloning site Green=Tags(s)
  
  MVGPTRSKLREGSSDRPQSSCTGQARRRWSAATMEPQQERSAPQERTKWSLLQHFLLGGRKLPSGARNYA
  ARRIQSLNAQNYFQLEEVAKLLLLNRFQFLFTLLDHFREKVQALQMHRFSHRTLFGLAIFVGILHWLHLI
  TLFENDHHFSHLSSLEREMTFRTEMGLYYSYFKTIIEAPSFLEGLWMIMNDRLTEYPLVINTVKRFHLYP
  EVVIAYWYRTIIGIMNLFGIETKTCWNVTRMEPLNEVQSCEGLGDPACFYIGVIFILNGLMMGLFFIYST
  YLSGSQLGGLITVACYFFNHGEATRVMWTPPLRESFSYPFLVLQMYILTIILRTSTVHKKHYMALCFSNV
  AFMLPWQFAQFILFTQIASLFPMYVVGYIEPSKFQKIIYVNMSSVALCFILMFGNSMYLSSYYSSCLLVT
  WAIMQKKSKIQKLGGTELQFWLIQGCFWWCGTIILKFLTSKICGVSDHIRLSDLIAARILRYTDFDTLIY
  TCAPEFDFMEQATPLRYIKTLLLPLILVITYLIFKKIVRDIMCVLYTNTYVRKQLLDNAELIFHTLQLLA
  FTGLAILIMRLKLFLTPHMCIMASLICSQRLFGWLFCRIHFENVVFGILTMMSIQGCANLHNQWSIMGEF
  TNLPQEELIHWIKHSTRPDAVFAGAMPTMASIKLSTLRPIVNHPHYEDADLRARTKIVYSVYSRKSAVEV
  RNNLLKLHVNYYVLEEAWCVVRTKPGCSMLEIWDVEDPSNAANPPLCSILLKDSRPYFTTVFQNSMYRVL
  KIN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166207
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166207.1, NP_001159679.1
• RefSeq Size: 3441 bp
• RefSeq ORF: 2322 bp
• Locus ID: 320752
• UniProt ID: P0CW70
• Cytogenetics: 9 A4
• Gene Summary: Probable C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins. Required during spermatogenesis for sperm head elongation and acrosome formation (By similarity).[UniProtKB/Swiss-Prot Function]