4932411N23Rik (NM_177705) Mouse Tagged ORF Clone

CAT#: MG214668

4932411N23Rik (tGFP-tagged) - Mouse RIKEN cDNA 4932411N23 gene (4932411N23Rik), (10ug)

Product Images

Circular map for MG214668

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 4932411N23Rik
• Synonyms: RP23-267A6.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214668 representing NM_177705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCTGTCTTACTTAGTGAGGACTCAGACAGGAACCTCATGCTTTGCCTTCAGAATGAAGCTAATG
  AAATGGAAAATCCTACTGAACCTCTGAATAAGAGGCCTTGTTTGTCCTCTGAAGTTGATTCTACTTCGTA
  TATGTCAGCTCTTGAATTCCCACTACAAGAAATGGAGCAAAGTTTTGGTTTGGTCACAACCACAGCCACA
  GAGGTGGCTGAGGAAGAGTTTGGTGGGGAAAATATGATGCAATTCGAGGTTTTAAAAGATGACAAACAAT
  ATGAAGTTTTCCCCTTGCCTAACAAGGAAGAGTCAGTAGTTAGCCAAGCATGGTTCACAAGTAAGGAAGC
  TAAGGATGCTCTAACTCATCAAGGACAGAAATGGAAGCAGGGGATGTGGTCCAAGGAAGAAACAGCTATT
  CTGATGAACAACATTGAAAGGTATATGAAGGACCATGGAGTAGAGAATCCTGCAGAAATCATTTTTAAGA
  TGGCAAAAGGTAAAAGAAAAGATTTCTACAGGTCTGTATCATTGGGTCTGAATAGACCTTTGTTTTCAGT
  TTATAGAAGAGTGGTCCGAATGTATGATGACAGAAACCATGTGGGTAAGTACAGCCCTGAGGAAATAGAG
  AAGCTCAAGGAGCTCTGGCAGAAGCATGGCAATGACTGGATAACAATAGGGGCCGCCATGGGAAGAAGTC
  CATCTTCGGTCAAAGACCGGTGCCGACTGATGAAGGATACTTGCAACACGGGGAAATGGACCGAAGAAGA
  AGAACAGCTTCTTGGCGATGTGGTTCATGAATTGACATGCACTGAGGTGGATGAGAAAGTTACCCATGGT
  GTGTGTTGGGCAACAGTGGCTCAACGAGTGGGTACTCGCTCAGCAAAGCAGTGTCGTGCTAAATGGCTCA
  ACTACTTGAACTGGAAACAGACTGGTGGTATTGAGTGGACAAGGAAAGATGAAGTCACTCTCATCCAAAG
  GCTAGTAGAGCTTGATGTATCCGATGAAAGTGAAATTCGCTGGGATGAATTAGCTAAAGGATGGGAAAGT
  GTTCGTTCACCACAATGGCTGCGAAATAAATGGTGGATCATCAAAAGGCAAATTACAAATCATAAAGATT
  TTGCATTCCCAGTCCTAGTAAGATGTCTTCAACAAGAATATGAAAGCCAAAATGCCAGCCTCAGGTTTTG
  GGAGAATAAATCAGGATCTGAAGTACCAGATAGCAACCCTGATATCATTTTTCAACAAGTCCCCTTTGGA
  GTCACAAGTATAGAAAATAATAATGCATCCATCTGTACTGACCCCATGACAGGATTGCAAATTTCATTCC
  AGCTCATCCCCAGACCCACAACAGAATTACCTGATGCTGTTGTTGACTCTGAAACCATAACCCTGCCCAC
  TGGACCACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214668 representing NM_177705
  Red=Cloning site Green=Tags(s)
  
  MSSVLLSEDSDRNLMLCLQNEANEMENPTEPLNKRPCLSSEVDSTSYMSALEFPLQEMEQSFGLVTTTAT
  EVAEEEFGGENMMQFEVLKDDKQYEVFPLPNKEESVVSQAWFTSKEAKDALTHQGQKWKQGMWSKEETAI
  LMNNIERYMKDHGVENPAEIIFKMAKGKRKDFYRSVSLGLNRPLFSVYRRVVRMYDDRNHVGKYSPEEIE
  KLKELWQKHGNDWITIGAAMGRSPSSVKDRCRLMKDTCNTGKWTEEEEQLLGDVVHELTCTEVDEKVTHG
  VCWATVAQRVGTRSAKQCRAKWLNYLNWKQTGGIEWTRKDEVTLIQRLVELDVSDESEIRWDELAKGWES
  VRSPQWLRNKWWIIKRQITNHKDFAFPVLVRCLQQEYESQNASLRFWENKSGSEVPDSNPDIIFQQVPFG
  VTSIENNNASICTDPMTGLQISFQLIPRPTTELPDAVVDSETITLPTGPQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177705
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177705.3, NP_808373.1
• RefSeq Size: 3634 bp
• RefSeq ORF: 1413 bp
• Locus ID: 237029
• Cytogenetics: X E3