Akap11 (NM_001164503) Mouse Tagged ORF Clone

CAT#: MG214493

Akap11 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 11 (Akap11), (10ug)

Product Images

Circular map for MG214493

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Akap11
• Synonyms: 6330501D17Rik; AKAP220; Gm80; mKIAA0629; PRKA11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214493 representing NM_001164503
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTTTCCAGCCCCTCAGGAGTAGTCACCTAAAGAGCAAAGCGTCTGTCCGAAAAAGCTTCAGTG
  AAGATGTGTTCCGGTCTGTAAAGTCTTTATTACAGAGCGAGAAGGAGCTATGCAGTGTATCAGGAGGAGA
  GTGTTTAAACCAGGATGAGCACCCCCAGTTAACTGAGGTCACGTTTCTGGGCTTTAATGAAGAAACAGAT
  GCTGCTCATATACAGGATCTAGCTGCAGTTTCATTGGAACTTCCAGATCTTCTGAATTCGCTCCATTTTT
  GCAGTCTAAGTGAAAATGAAATCATTTGTATGAAGGATACCAGTAAATCGTCCAATGTAAGCAGTAGTCC
  TCTAAATCAGAGTCATCATTCGGGAATGCTTTGTGTCATGAGAGTGTCACCTACATTACCGGGACTCAGA
  ATTGATTTTATCTTTAGTCTCCTAAGTAAATATGCTGCTGGCATAAGACACACCCTGGACATGCACGCGC
  ATCCGCAGCACCACCTTGAGACCACTGATGAAGATGATGATGATACCAACCAGTCTGTGTCTTCCATAGA
  GGATGACTTTGTCACTGCTTTTGAGCAGTTAGAGGAGGAAGAAAATGCAAAGCTATATAATGATGAAATA
  AATATTGCTACACTAAGGAGCCGGTGTGATGCTGCTTCTCAGACTACTTCAGGCCACCATTTAGAAAGCC
  ATGATTTAAAAGTGCTGGTTAGTTATGGATCACCGAAGTCACTGGCTAAGCCCTCACCTTCAGTAAATGT
  ACTGGGACGCAAGGAAGCTGCTTCTGTGAAAACATCTGTTACAACATCGGTTTCAGAGCCCTGGACCCAG
  AGGAGTCTCTACAGGTGCCCCAGTGCCTCCGATAAAGACAAGGACACACAGGAGACATTCTTTCCTTCTC
  CTGTCTACTCCTCAGAGTCTGAGTGCTCAAGCCCAAGTCCTGTGATTTTTTTGGATGAAGAGGGATATCA
  AAAAAGTTTAAAAGCGAAACTTGAGCTGCCTGAAATTCCTGTGACAAAAGATGATGTAGAAGATTCAGAC
  TCAGAAGTGAGTGAATTTTTTGATAGCTTTGATCAGTTTGATGAACTAGAGCAAACTTTGGAGACTTACT
  TGCTCATGGAAGATCCTATCATAGGGAAGTCATCACAGAAGATAGGGCACAAATATGAAAAATCTTGTAT
  GAATCCTCAGAAATTCAAGTTTGAGCGTCCAGCTCTTCCAGCTAATGTTAGAAAGCCAACTCCTCGCAAA
  CCTGAGTCTCCGTATGGTCACCTGTCTGATGCTCCAGATTCCCCTCGTCCGGTGATGACATCAGAGGATA
  GTGGCTTGTTCAGCCCTATTCGAACCTCAGCTTTTAGTCCTCCTGGAAGCTGTACTCCTGCTGAATGGTT
  TTGCCAAACAGATACTAGTGAAAATAGGAATCATGAAAATTACTGCACTTATGAGGATTATGCAAATAAT
  CTTTCGTGTGAAGTACTAGGCTCAGTTCTTCATACTCAACATGCAAACACAATGTCAGATATTAATAGTA
  TTAAAAGGGGAAGAAATCATACGGTAACTTTTAAGTATGGAGACTCTGATCAAAAAAGTAAATGTAAAAA
  ATCTTCAGTGATTACAGGTAGCGTTCAGAAGTTTGCAGCAGATCTTGTGGGGAAAAGTTTGGGCAGTGCG
  TTTAAAGACTTACAGAAAGGAGTTTCTTCATGTACCAATACCTTATGCCACTTAGCTGTCAAATTGACTT
  CGTCTGTATTTCAGATGGCATTTAATGAACTTAGAAGGCAGTGTGCATTTTCACTAAAAGAACGAGCCAT
  TGGTAGCCTGGCCAGTCTTTTGGTGAGTGATGCCTTATCAAATGCCTTGAAAGACTTACAGTATGTAAAA
  AAGCAGATGTTTACAAACACAGTTGCTAGGTTTGCTGCGGACCTTGCTGAAGAACTTGTTTTTGAAGGTA
  TCATGGAAGTGTGTCAGTTTTCATGTCCTCAAACACCTGCATCTTCACAGTGTCAGTCATTTGACTTTGA
  AGACAAAGTCGTGAAGTCCTATGCCAAAGATTTGTCTGAATCTGTAATACAAGAAGCATTCATAGAACTG
  TCACAGACTAATGTGACGTTTACAACAAAGGCAGCAGTTAATGTTTCTATGGGTAATGTCAAGTATGTGA
  GTGCAGAAAGTGTATCACCTACGCAGACCTTTACATTTTCCTCATCCTTCAGCGGTCAAGCAGTTATGAT
  GACAAAACCAATGCAGGAGCATAAGAAGGAATACACAGTGCAGCAGGCTTTGTTTTGTACCTCAGGAATT
  GTCACTTCTATACCAGTGCCCTTGGCCGGTAGTGCCCTTCTCCCGTATCATATGTCATCTACTTTGTATC
  CTTCCAAGTGTCTTTCATCTGAGCCTAGTAAAGCAAGTGGTGGCTCTACCCAAGAACACATTGCCATAGA
  ACGCAGTGCAGAGGAAGTAGATTGTCTCAGAAGTACCTGTCTACCTTCAGAACTCAATCCATGCAACCAG
  AATGACTTCAAACCAACTAATGGTGATATTGACAGGCAGAGTCCTTCAAAGTTAATGAGTGGCCCTGTAA
  TTATTAGCAACTTTTCCGCAGCAATGGTACATACAATAGTAAATGAAACCTTAGAGTCCATGACGTCCTT
  CAAAGCTACAAAAACTATTGATACAAATGCAGATTATTTAACTAAAACAATAAAGGGAAAAGCCTGTTCT
  CCTTCCCTTTGTGACCAAGCAGCACCACAGGAGAACAAAGCTAGCAGTAAGGACATGTTTGCTGAGCAGT
  TATCAAAGTCTATTATTAAACATTCCCTGGATAAAAGCAAGTCAATGCTTCCAAATAGAGATAAAAAACC
  CGGTAGTAAGGAACACGTGCTTGTTCTTGGAGAAGAATCACAGATGACCTTGGGGGAAACTCCCAAATTC
  CTTGACTTTTCAGATAACTCACCTCATCGTTCACTCCTGGTTGGAAACTATTGTGTCGCAGAATGTAAAG
  ATTCTGTGGGTTTTGGATTTTCTCTAGAGGCACTGCCACCTTGTTCAATGATGACAAATCAGAAATCTGA
  CTTAAAGGAAGTTGTGAAGGACAAAGAAGTGACAAGGCACAATTTGAATAATACTGCACTTGAGCCCATG
  CCTTTTGGACAGGAAAGCTCTTTCCGTCACTCACAGACTTTCTCATCTGCAGTGCTTACCTGTGTAGATG
  GTTTGCATGAGGAAGATAAGCAGAAAATCCGAGACAGAAATGTAATACCTGATACTCCCCCATCGACTCC
  TTTAGTGCCATCCCAGACTAGTTCTGAATGGGACATCAAAACATTAAGCAAACAACTCAAGGGGGAGTTA
  GCCAAAGAATTTGCACCCGCAACACCACCTTCAACGCCTCACAACTCATCTGTTGGTAGTTTGTCTGAGA
  ATGAACAAACTACTATAGAAAAGGAAGAATTTATGTTGAAGCTCATGCGCTCTCTTTCAGAAGAAGTTGA
  GAGCAGTGAAGGTGAAGAACACCCAGGAATGCATGTAAAGGCAGAGCACCCGGGGAAAAAAGTCCAGTTT
  GCAGAAGCATTCGCCACACACATCATTTCTCTTGCAACTGAAGCGGCAGCCTCCCACTTAGATCATGAGA
  CTACTCAAGAATTCAAGGTTCAAAACCCTCACTTAAATGTACCAAGTCAAAGAAATGTGCTGCCAGCTTT
  AAGTCATTCCGATGAAAGCATACAGACGTGCACTTTTGCAAGTGATATGGCAGCTGATGTCATTGCAGAA
  GCCGAGAAGATTGCAAACGCTAGAAGCTGTATGCTTTTCAGGCATGAGAGGAACATTTGTCATGTTGAAG
  GTGGCCGAGGTAAAGCAGAAGAGAAACTGGATGTGGAAGATGTTGCACATCCAAGAGAAGTCGATACTTG
  TGTTCTTTCATTACCAAGTGGCATGCCAGGTCTGACATACAAGTATCCCAGTTGTGAAAGTGTGACTGAT
  GAGTATGCAGGGCATGTTATTCAGGTGCTCCAACAGCAAGGTGGCAGTGGAGAGCTAATCATGGAGCAGT
  ATGCCAGCAGACTGGCCTACCGGTCTGTCACTGCAGCAGCTCGGGAAGCAGCTAAGACAGTGAAAATGAA
  GTGCGGTTCGAAACTCTTCCCTCTGCACGGCTGTCATGGGAAAACAAACAAGGAACTGTTGGTGTTCTCA
  AGTAAAGAGCGTCACCAAGAAGTCGATAGACAAAGAAAAAGAAATGGCAGCCATCTTTGCAAATACCAGA
  CATGTGAAAGGACACAGGATCCGTGTAGAAATGAACTTTCTGAACTGTACAGTTTTTCAGCCTCTCTTGC
  TAGCAGCATAACAAGAGATGCAAAGAAACAGCTCACAGCACCTAAAGTTGACTTGCCAAAATCCTCAACA
  GATGGTTGTTTTTTTGAAAAATCTGAATGTGTTGACAGCAGTGAGAATGTTACTGGACCAGAACGTTCAA
  AGTCTTGTCAACCTTTGCAAAGCCATGGATTTTGCCAGAATACAGGCTATCTAAGTGGATATAGTTGTGC
  AGAGAATGCTCAAGCTATAGAACAGTATGCTAGAAAAGTAGTGGGTGACACTTTAGAGCTAAGTTTAGGA
  CCCACAGTTTTTCATAATTCAGAGACCACAGCATCAGCAGATAGAATTACTTACGCAGAAAAATTGTCAC
  CGCTTATAAATGAAGCTTGCAGATACTGTGACCTTAAGGAATTCCATGGTTGCACCAGAAATTCTGCTCA
  GCTCTTTTCAAAGCAGAGTCCGTGTGCTAGTGCTAAGCCAAGTTCACGTTCAAAACTTAGTAGCATTCGT
  CAGAAATCAAGAATTTTTCATCTTGATGTACCTCAGATTCATGTTAATCTTGATAAAAGGGCAGTGCTTG
  CTGAGAAGATAGTTGCTGAAGCTATTGAGAAAGCAGAGAGAGAGTTGAGCAACACGAGTTTGGCAGCTGA
  TAGTGGAATCGGACAAGATGGCATTAGCTTTGCTGAGAGCCTTACTACAGAAATCATGACAACAGCTGTG
  ACAAATGCTGGGCATGCTGTTAGCAGTTCAAGAGAAATAGAAGATTTTCAGTCAACTGAGTCACTTGGCA
  GCCAGCAGATGAATCTCAGTGTTGGTGAAGACAGCACAGGTAGCTGGTCCAACTTAAGTTTTGAGGATGA
  CCATCAAGATGAAAGCAGCAGTTTCCATCATCTCAGTGAAAGTAATGGTAACAGCAGTAGCTGGAGCAGT
  CTTGGTTTAGAAGGAGATTTGTATGAGGACAATTTATCCTTTCCAACATCAGACAGTGATGGATCGGATG
  ATGGAGATGACGAGCAGGAGGATGGTGTGGAAGATTTGCAGCAAAATGGAAAGACTCTGCTAATTATGAA
  CATTGACATGGAGCCTGGAGCTGTAGACCCGCAATTAAGGATTATTCTTCAGTGGCTCATTGCCTCTGAG
  GCTGAAGTTGCAGAACTTTATTTTCAGGACTCTGCAAAGAAGGAGTTTATATTACTTTCAAAGCAGTTAC
  AAGAGAAAGGATGGAAAGTGGGAGATGTCCTGCAGGCTGTGCTTAAATACTACGAAGTGGTGGAAAAGCC
  CTCAAGGGAGGAAAGATGCAAGTCGCTGTTTGATTGGCTTTTAGAAAATGCCCAGAACAGACTCCAGACC
  AGTATGCTTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214493 representing NM_001164503
  Red=Cloning site Green=Tags(s)
  
  MAAFQPLRSSHLKSKASVRKSFSEDVFRSVKSLLQSEKELCSVSGGECLNQDEHPQLTEVTFLGFNEETD
  AAHIQDLAAVSLELPDLLNSLHFCSLSENEIICMKDTSKSSNVSSSPLNQSHHSGMLCVMRVSPTLPGLR
  IDFIFSLLSKYAAGIRHTLDMHAHPQHHLETTDEDDDDTNQSVSSIEDDFVTAFEQLEEEENAKLYNDEI
  NIATLRSRCDAASQTTSGHHLESHDLKVLVSYGSPKSLAKPSPSVNVLGRKEAASVKTSVTTSVSEPWTQ
  RSLYRCPSASDKDKDTQETFFPSPVYSSESECSSPSPVIFLDEEGYQKSLKAKLELPEIPVTKDDVEDSD
  SEVSEFFDSFDQFDELEQTLETYLLMEDPIIGKSSQKIGHKYEKSCMNPQKFKFERPALPANVRKPTPRK
  PESPYGHLSDAPDSPRPVMTSEDSGLFSPIRTSAFSPPGSCTPAEWFCQTDTSENRNHENYCTYEDYANN
  LSCEVLGSVLHTQHANTMSDINSIKRGRNHTVTFKYGDSDQKSKCKKSSVITGSVQKFAADLVGKSLGSA
  FKDLQKGVSSCTNTLCHLAVKLTSSVFQMAFNELRRQCAFSLKERAIGSLASLLVSDALSNALKDLQYVK
  KQMFTNTVARFAADLAEELVFEGIMEVCQFSCPQTPASSQCQSFDFEDKVVKSYAKDLSESVIQEAFIEL
  SQTNVTFTTKAAVNVSMGNVKYVSAESVSPTQTFTFSSSFSGQAVMMTKPMQEHKKEYTVQQALFCTSGI
  VTSIPVPLAGSALLPYHMSSTLYPSKCLSSEPSKASGGSTQEHIAIERSAEEVDCLRSTCLPSELNPCNQ
  NDFKPTNGDIDRQSPSKLMSGPVIISNFSAAMVHTIVNETLESMTSFKATKTIDTNADYLTKTIKGKACS
  PSLCDQAAPQENKASSKDMFAEQLSKSIIKHSLDKSKSMLPNRDKKPGSKEHVLVLGEESQMTLGETPKF
  LDFSDNSPHRSLLVGNYCVAECKDSVGFGFSLEALPPCSMMTNQKSDLKEVVKDKEVTRHNLNNTALEPM
  PFGQESSFRHSQTFSSAVLTCVDGLHEEDKQKIRDRNVIPDTPPSTPLVPSQTSSEWDIKTLSKQLKGEL
  AKEFAPATPPSTPHNSSVGSLSENEQTTIEKEEFMLKLMRSLSEEVESSEGEEHPGMHVKAEHPGKKVQF
  AEAFATHIISLATEAAASHLDHETTQEFKVQNPHLNVPSQRNVLPALSHSDESIQTCTFASDMAADVIAE
  AEKIANARSCMLFRHERNICHVEGGRGKAEEKLDVEDVAHPREVDTCVLSLPSGMPGLTYKYPSCESVTD
  EYAGHVIQVLQQQGGSGELIMEQYASRLAYRSVTAAAREAAKTVKMKCGSKLFPLHGCHGKTNKELLVFS
  SKERHQEVDRQRKRNGSHLCKYQTCERTQDPCRNELSELYSFSASLASSITRDAKKQLTAPKVDLPKSST
  DGCFFEKSECVDSSENVTGPERSKSCQPLQSHGFCQNTGYLSGYSCAENAQAIEQYARKVVGDTLELSLG
  PTVFHNSETTASADRITYAEKLSPLINEACRYCDLKEFHGCTRNSAQLFSKQSPCASAKPSSRSKLSSIR
  QKSRIFHLDVPQIHVNLDKRAVLAEKIVAEAIEKAERELSNTSLAADSGIGQDGISFAESLTTEIMTTAV
  TNAGHAVSSSREIEDFQSTESLGSQQMNLSVGEDSTGSWSNLSFEDDHQDESSSFHHLSESNGNSSSWSS
  LGLEGDLYEDNLSFPTSDSDGSDDGDDEQEDGVEDLQQNGKTLLIMNIDMEPGAVDPQLRIILQWLIASE
  AEVAELYFQDSAKKEFILLSKQLQEKGWKVGDVLQAVLKYYEVVEKPSREERCKSLFDWLLENAQNRLQT
  SMLV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164503
• ORF Size: 5682 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164503.1, NP_001157975.1
• RefSeq Size: 9416 bp
• RefSeq ORF: 5685 bp
• Locus ID: 219181
• Cytogenetics: 14 D3