Gm136 (NM_001033255) Mouse Tagged ORF Clone

CAT#: MG214286

Gm136 (tGFP-tagged) - Mouse predicted gene 136 (Gm136), (10ug)

Product Images

Circular map for MG214286

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gm136
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214286 representing NM_001033255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAGAAATCCAAATTACACAGATTTTGTTTGCTGTGCCGTTTGCAATAAAATAATTCCACCAGCCC
  CTTTCGGGGAAACCTTCAAACGGATCTATGACTACAAGCCATTTAAGACACGCTTCTATACTCACAAAGA
  TATCTTGGATATTGGGGCAAGTATTCTGAACAAAGAAGAAGAGTTTCGAGAGACTGTGTTCAAGGAACAA
  ATCAAAAAAGCAGAAGCCAAAGTGTGGGAAAAGGCTGAACTGCTCCAAAAACAAGCCGTGGATCAAGCAG
  TTGAAGACGCAGAAGCCAGACACAAATTTGAAATTCGGGTTCTGGAAGAACAACATCAAAAAGACTTAAA
  GGCTCTAGAAGACAAAACCAAGGTGAATATGATTCAACAGATGAAGGAAGAGCTGAACAGAGAGCACACA
  GCTGCAGAACAGCGTATGGTCCATCGGATCCAGAGGATTATGATGGAGTGTCACCAGGAGAAGATGGAGG
  CTGTGAAGAAAGCCAGGGAAGAAGAGAGAAGGATTGCTCAGAAAGCTATCGAAGAAGAGAAAAGCAAGGT
  CCTGGAGGAATTTGTAACCACTGGCGTAACCGTCATTAAAGACAAGAAGACAAGCCTGGGCCAGCTGATC
  AAAGCAAAGGAGCACGAGATGACCATCTACTACGGCATGGCTCAGAGGCAGAAGCAAGAAGAAGTACAAG
  AAGTGCTTCAAGAGGCGGAAAAAACACACCAGGCCACTCTTGACAATATGATGGGCAAACTTGTCAATAC
  CCAGGGGGAGCTGCTCTCTGTGGCCAAGCAACTGGGAATCATGACAAACTGGAAAGATTTCCTGGAGGAG
  GAACTGCAGGAGACCAGGGCCGCGTTTCAGAAGTATATCAACTACACCTTCCCCAGGCTCTCCCCAGGAC
  ACGCCGACTTCATTCTGCCCGAAAGAAAGAAAACACCTTCTATCCTTGCTAAGGAAAATGAGCCGAGGAC
  TGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214286 representing NM_001033255
  Red=Cloning site Green=Tags(s)
  
  MIRNPNYTDFVCCAVCNKIIPPAPFGETFKRIYDYKPFKTRFYTHKDILDIGASILNKEEEFRETVFKEQ
  IKKAEAKVWEKAELLQKQAVDQAVEDAEARHKFEIRVLEEQHQKDLKALEDKTKVNMIQQMKEELNREHT
  AAEQRMVHRIQRIMMECHQEKMEAVKKAREEERRIAQKAIEEEKSKVLEEFVTTGVTVIKDKKTSLGQLI
  KAKEHEMTIYYGMAQRQKQEEVQEVLQEAEKTHQATLDNMMGKLVNTQGELLSVAKQLGIMTNWKDFLEE
  ELQETRAAFQKYINYTFPRLSPGHADFILPERKKTPSILAKENEPRTD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033255
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033255.2, NP_001028427.1
• RefSeq Size: 1303 bp
• RefSeq ORF: 987 bp
• Locus ID: 214568
• UniProt ID: Q3V037
• Cytogenetics: 4 A5