Pcdh7 (NM_018764) Mouse Tagged ORF Clone

CAT#: MG214198

Pcdh7 (tGFP-tagged) - Mouse protocadherin 7 (Pcdh7) transcript variant 2, (10ug)

Product Images

Circular map for MG214198

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pcdh7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214198 representing NM_018764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGATGCGGACCACGGGATGGGCGCGCGGCTGGTGCTTGGGCTGTTGTCTCCTTCTGCCGCTCT
  GCTTCAGCCTGGCCGCCGCCAAGCAGCTGCTCCGGTACCGGCTGGCCGAGGAGGGCCCCGCCGACGTGCG
  GATCGGCAATGTGGCCTCGGACCTGGGCATCGTGACCGGCTCCGGCGAGGTGACTTTCAGCCTTGAGTCT
  GGTTCTGAGTATCTGAAGATTGACAACCTCACCGGCGAGCTGAGCACCAGTGAGCGGCGCATCGACCGAG
  AAAAGCTGCCCCAATGTCAGATGATCTTCGACGAGAACGAATGTTTCCTGGACTTCGAGGTGTCGGTGAT
  AGGGCCCTCACAGAGCTGGGTGGACCTGTTTGAGGGTCGGGTCATCGTGCTGGACATCAACGATAACACG
  CCCACCTTCCCGTCGCCGGTGCTCACGCTCACGGTGGAGGAGAACCGGCCTGTAGGCACGCTCTACCTGC
  TGCCCACGGCCACCGATCGTGACTTTGGTCGCAATGGCATCGAACGCTACGAGCTGCTCCAGGAGCCCGG
  GGGTGGCGGCGGCAGCGGCGAGGGAAGGCGCTTGGGGCCGGCGGACAGCGCCCCCTACCCAGGGGGCGGC
  GGGAACAGCGCGAGCGGCGGCGGCTCTGGAGGCTCCAAGCGGCGGCTGGACGCGCCTGAGGGTGGCGGCG
  GGACGAGTCCTAGTGGCCGAAGCAGTGTGTTTGAGCTTCAGGTGGCAGACACTCCAGACGGCGAGAAACA
  ACCGCAGCTGATCGTGAAGGGGGCGCTGGACCGGGAGCAGAGAGATTCCTATGAGCTGACCCTCCGAGTG
  CGCGATGGGGGCGACCCACCTCGGTCTTCGCAGGCCATCTTGCGGGTGCTCATCACCGACGTGAATGACA
  ACAGCCCCCGCTTCGAGAAGAGCGTGTATGAGGCTGACCTGGCTGAGAACAGCGCTCCGGGCACCCCCAT
  CCTACAGTTGCGCGCCACCGATTTGGATGTAGGGGTCAATGGACAGATCGAGTATGTATTTGGGGCTGCC
  ACCGAGTCGGTAAGACGGCTACTGCGCCTGGATGAAACGTCGGGCTGGCTCAGTGTCTTACACCGGATCG
  ACCGCGAGGAAGTGAACCAGTTGAGATTCACAGTAATGGCCCGTGACCGCGGGCAGCCCCCCAAGACTGA
  TAAGGCCACCGTAGTCCTCAACATCAAAGACGAGAACGACAACGTTCCCTCCATCGAAATTCGCAAGATA
  GGGCGCATCCCACTTAAGGACGGGGTGGCCAACGTAGCCGAAGATGTCCTGGTGGACACCCCCATCGCCC
  TGGTTCAGGTGTCCGACCGTGACCAAGGCGAGAACGGGGTAGTCACCTGTACCGTAGTGGGAGACGTTCC
  TTTCCAGCTTAAGCCAGCCAGCGACACAGAAGGCGATCAAAACAAGAAAAAGTACTTCCTGCACACATCA
  GCCCCACTAGACTATGAGACCACCCGGGAGTTCAACGTGGTCATAGTAGCTGTGGACTCTGGCAGTCCCA
  GCCTCTCCAGCAACAATTCCTTGGTGGTCAAGGTGGGAGACACTAATGACAACCCTCCTGTCTTTGGCCA
  GTCTGTGGTTGAGGTTTACTTTCCAGAGAACAACATTCCCGGGGAGAGGGTAGCCACAGTGCTGGCGACA
  GATGCTGACAGCGGGAAGAATGCAGAGATCGCCTATTCACTGGACTCCTCAGTGATGGGGACTTTTGCCA
  TCGATCCCGATTCTGGAGACATCCTGGTCAATACAGTACTGGACCGGGAGCAGACTGACAGGTATGAGTT
  TAAAGTTAATGCCAAAGACAAAGGCATCCCTGTGCTGCAAGGCAGCACCACGGTGATTGTACAGGTGGCT
  GATAAAAACGACAACGACCCTAAGTTTATGCAAGATGTCTTTACCTTTTATGTGAAGGAAAACTTACAAC
  CCAACAGCCCTGTGGGGATGGTCACCGTGATGGATGCTGACAAGGGACGGAATGCAGAGATGAGCCTGTA
  CATAGAGGAGAACAGTAACATTTTTTCTATTGAAAATGACACAGGGACCATTTACTCCACAATGTCTTTT
  GACAGGGAACATCAGACTACGTACACTTTCAGAGTGAAGGCTGTGGATGGGGGAGATCCTCCCAGGTCGG
  CCACAGCCACAGTCTCTCTCTTTGTGATGGATGAAAATGACAACGCTCCCACAGTTACCCTTCCCAGAAA
  CATTTCCTACACGTTGCTGCCACCTTCAAGTAATGTCAGGACAGTAGTAGCTACAGTGTTAGCGACAGAC
  AGTGATGATGGCATCAATGCGGACTTGAACTACAGCATTGTGGGAGGGAATCCTTTCAAGCTGTTTGAGA
  TTGATTCCACCAGTGGTGTGGTTTCCTTAGTGGGGAAACTCACCCAGAAGCATTATGGCTTGCACAGGCT
  GGTTGTGCAAGTGAATGACAGTGGCCAGCCTTCCCAGTCCACTACGACTTTGGTGCATGTATTTGTCAAT
  GAAAGTGTTTCCAATGCAACTGTAATTGACTCTCAGATAGTCCGAAGTTTGCATACCCCACTCACCCAGG
  ATATAGCTGGTGACCCAAGCTATGAAATTAGCAAACAGAGACTTAGTATTGTCATTGGGGTGGTTGCTGG
  CATTATGACAGTGATTCTAATCATTTTAATTGTCATGATGGCAAGATACTGCAGGTCCAAAAATAAAAAT
  GGCTACGAAGCTGGCAAAAAAGACCATGAAGACTTTTTTACACCCCAGCAGCATGACAAATCTAAAAAGC
  CTAAAAAGGACAAGAAAAACAAAAAATCTAAGCAGCCACTCTACAGCAGTATCGTCACTGTCGAAGCTTC
  TAAACCAAATGGACAGAGGTATGATAGTGTCAATGAGAAGCTGTCAGACAGCCCAAGCATGGGCCGATAC
  CGATCTGTTAATGGTGGGCCTGGCAGTCCCGACCTGGCAAGGCATTACAAATCCAGTTCCCCATTGCCTA
  CTGTCCAGCTTCACCCCCAGTCACCAACTGCAGGGAAAAAACATCAAGCTGTACAAGATCTACCACCAGC
  CAACACATTTGTGGGAGCAGGAGACAACATTTCAATTGGATCAGATCACTGCTCCGAGTACAGCTGTCAA
  ACAAGTAACAAGTACAGCAAACAGATGCGTGTACATCCATACATTACGGTGTTTGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214198 representing NM_018764
  Red=Cloning site Green=Tags(s)
  
  MLRMRTTGWARGWCLGCCLLLPLCFSLAAAKQLLRYRLAEEGPADVRIGNVASDLGIVTGSGEVTFSLES
  GSEYLKIDNLTGELSTSERRIDREKLPQCQMIFDENECFLDFEVSVIGPSQSWVDLFEGRVIVLDINDNT
  PTFPSPVLTLTVEENRPVGTLYLLPTATDRDFGRNGIERYELLQEPGGGGGSGEGRRLGPADSAPYPGGG
  GNSASGGGSGGSKRRLDAPEGGGGTSPSGRSSVFELQVADTPDGEKQPQLIVKGALDREQRDSYELTLRV
  RDGGDPPRSSQAILRVLITDVNDNSPRFEKSVYEADLAENSAPGTPILQLRATDLDVGVNGQIEYVFGAA
  TESVRRLLRLDETSGWLSVLHRIDREEVNQLRFTVMARDRGQPPKTDKATVVLNIKDENDNVPSIEIRKI
  GRIPLKDGVANVAEDVLVDTPIALVQVSDRDQGENGVVTCTVVGDVPFQLKPASDTEGDQNKKKYFLHTS
  APLDYETTREFNVVIVAVDSGSPSLSSNNSLVVKVGDTNDNPPVFGQSVVEVYFPENNIPGERVATVLAT
  DADSGKNAEIAYSLDSSVMGTFAIDPDSGDILVNTVLDREQTDRYEFKVNAKDKGIPVLQGSTTVIVQVA
  DKNDNDPKFMQDVFTFYVKENLQPNSPVGMVTVMDADKGRNAEMSLYIEENSNIFSIENDTGTIYSTMSF
  DREHQTTYTFRVKAVDGGDPPRSATATVSLFVMDENDNAPTVTLPRNISYTLLPPSSNVRTVVATVLATD
  SDDGINADLNYSIVGGNPFKLFEIDSTSGVVSLVGKLTQKHYGLHRLVVQVNDSGQPSQSTTTLVHVFVN
  ESVSNATVIDSQIVRSLHTPLTQDIAGDPSYEISKQRLSIVIGVVAGIMTVILIILIVMMARYCRSKNKN
  GYEAGKKDHEDFFTPQQHDKSKKPKKDKKNKKSKQPLYSSIVTVEASKPNGQRYDSVNEKLSDSPSMGRY
  RSVNGGPGSPDLARHYKSSSPLPTVQLHPQSPTAGKKHQAVQDLPPANTFVGAGDNISIGSDHCSEYSCQ
  TSNKYSKQMRVHPYITVFG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018764
• ORF Size: 3207 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018764.2, NP_061234.2
• RefSeq Size: 5610 bp
• RefSeq ORF: 3210 bp
• Locus ID: 54216
• Cytogenetics: 5 C1