Armt1 (NM_024261) Mouse Tagged ORF Clone

CAT#: MG214194

1700052N19Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700052N19 gene (1700052N19Rik), (10ug)

Product Images

Circular map for MG214194

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Armt1
• Synonyms: 1700052N19Rik; AW320013; AW536799
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214194 representing NM_024261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGTCCCCGGCGTTTCTTTCCGCTAAGGACGAGGGGTCCTTTGCATATCTTACGATTAAGGACA
  GAACACCGCAGATCTTAACCAAGGTTATTGATACATTGCATCGACATAAAAGTGAATTTTTTGAAAAACA
  TGGAGAGGAAGGCATTGAAGCTGAAAAGAAAGCCATCTCTCTTCTTTCTAAATTACGGAATGAACTGCAG
  ACAGACAAACCAATCACCCCCTTGGTGGACAAGTGTGTTGACACCCACATATGGAATCAATACCTGGAGT
  ATCAGCGGAGCCTTTTAAATGAAGGCGATGGGGAGCCACGCTGGTTCTTCTCACCCTGGTTGTTTGTAGA
  GTGCTACATGTATCGGAGAATTCATGAAGCCATCATGCAGAGTCCGCCAATCCATGACTTCGACGTGTTT
  AAGGAAAGCAAAGAGGAGAACTTCTTCGAGTCGCAGGGCTCCATCGATGCTCTGTGTTCACACCTGCTGC
  AGCTGAAGCCCGTCAAAGGCCTCAGGGAAGAGCAGATCCAGGATGAGTTCTTTAAACTCCTGCAGATTTC
  CCTCTGGGGGAATAAGTGTGATCTGTCTCTCTCAGGTGGAGAAAGTAGTTCTCAGAAGGCCAATATAATA
  AATTGTTTGCAAGACCTAAAGCCATTCATTTTGATAAATGACACAGAATCTCTCTGGGCATTGCTCAGTA
  AGTTAAAGAAAACAGTAGAAACGCCCGTAGTTAGAGTAGACATTGTTCTGGATAATTCTGGCTTTGAACT
  GATTACGGATTTAGTATTGGCGGACTTTTTATTTTCCTCGGAGTTAGCTACTGAGATTCATTTCCATGGG
  AAAAGCATCCCATGGTTTGTGTCTGACGTTACTGAGCATGACTTTAATTGGATAGTTGAGCACATGAAGA
  GCAGCAATCTGGAGTCCATGTCCACCTGTGGGGCCTGCTGGGAGGCCTATGCTAGGATGGGGAGGTGGGC
  TTACCACGACCATGCATTTTGGACTCTGCCTCATCCATATTGTGTGATGCCCCAGGTCGCCCCTGACTTA
  TACGCTGAACTGCAAAAGGCACATCTTATTTTATTCAAGGGAGATTTGAATTATAGGAAGCTGATGGGTG
  ACAGAAAATGGAAGTTCACCTTTCCGTTCCATCAGGCTCTGAGTGGCTTCCACCCTGCACCTCTCTGTAG
  CATAAGGACATTAAAGTGCGAGCTTCAGGTTGGTCTGCAGCCTGGGCAGGCGGAGCAGCTCACGGCCTCT
  GATCCCCACTGGCTGACCACTGGCAGGTATGGAATATTGCAGTTTGATGGGCCACTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214194 representing NM_024261
  Red=Cloning site Green=Tags(s)
  
  MAESPAFLSAKDEGSFAYLTIKDRTPQILTKVIDTLHRHKSEFFEKHGEEGIEAEKKAISLLSKLRNELQ
  TDKPITPLVDKCVDTHIWNQYLEYQRSLLNEGDGEPRWFFSPWLFVECYMYRRIHEAIMQSPPIHDFDVF
  KESKEENFFESQGSIDALCSHLLQLKPVKGLREEQIQDEFFKLLQISLWGNKCDLSLSGGESSSQKANII
  NCLQDLKPFILINDTESLWALLSKLKKTVETPVVRVDIVLDNSGFELITDLVLADFLFSSELATEIHFHG
  KSIPWFVSDVTEHDFNWIVEHMKSSNLESMSTCGACWEAYARMGRWAYHDHAFWTLPHPYCVMPQVAPDL
  YAELQKAHLILFKGDLNYRKLMGDRKWKFTFPFHQALSGFHPAPLCSIRTLKCELQVGLQPGQAEQLTAS
  DPHWLTTGRYGILQFDGPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024261
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024261.2, NP_077223.2
• RefSeq Size: 2318 bp
• RefSeq ORF: 1320 bp
• Locus ID: 73419
• UniProt ID: A6H630
• Cytogenetics: 10 A1
• Gene Summary: Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate (By similarity). Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism (By similarity). Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues (By similarity). Possibly methylates PCNA, suggesting it is involved in the DNA damage response (By similarity).[UniProtKB/Swiss-Prot Function]