Prrt2 (NM_001102563) Mouse Tagged ORF Clone

CAT#: MG214094

Prrt2 (tGFP-tagged) - Mouse proline-rich transmembrane protein 2 (Prrt2), (10ug)

Product Images

Circular map for MG214094

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prrt2
• Synonyms: 1500031I19Rik; AI195361; DSPB3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214094 representing NM_001102563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCAGCAGCTCTCAGGTCTCTGAGATGAAGGGGGTGGAAGACAGTTCCAAAACCCAGACAGAAG
  GTCCCAGGCATTCTGAAGAAGGACTAGGTCCTGTCCAGGTTGTAGCAGAGATCCCAGACCAGCCAGAGGC
  CCTTCAGCCGGGCCCAGGCATCACTGCAGCCCCTGTGGACTCAGGGCCTAAGGCAGAGCTGGCACCAGAA
  ACCACAGAGACCCCAGTTGAAACCCCAGAAACAGTCCAGGCCACAGACCTCAGTTTAAACCCAGAAGAAG
  GCTCCAAGGCCAGCCCCAGCCCCAGCCCCAGCGAGGCACGCCAAGAGCCAGCATCCAAACCAGATGTGAA
  CAGAGAGACTGCAGCAGAGGAAGGGTCTGAGCCGCAGTCTACAGCCCCTCCTGAGCCAACCTCGGAGCCT
  GCTTTTCAGATAAACACCCAGTCAGACCCTCAGCCAACTTCCCAGCCTCCTCCTAAACCACCCCTTCAGG
  CAGAGCCCCCCACCCAAGAGGACCCTACCACAGAGGTCCTGACAGAAAGTACAGGGGAAAAACAAGAAAA
  CGGAGCAGTGGTTCCCCTTCAGGCTGGTGACGGGGAAGAGGGCCCAGCCCCCCAACCTCACTCACCACCC
  TCAACTAAAACACCCCCAGCCAATGGGGCTCCCCCCCGTGTGCTGCAGAAACTCGTTGAGGAAGACAGAA
  TAGGAAGGGCACACGGTGGGCATCCAGGATCTCCTCGAGGTAGCCTAAGCCGTCATCCCAGCTCCCAGCT
  GGCAGGGCCTGGGGTGGAAGGGGGCGAAGGCACCCAGAAACCTCGGGACTATATCATCCTTGCCATCCTG
  TCCTGCTTCTGCCCCATGTGGCCTGTCAACATTGTGGCCTTCGCTTATGCCGTCATGTCCCGGAACAGCC
  TGCAACAGGGGGACGTGGATGGGGCTCAACGTCTGGGCCGAGTAGCCAAGCTCTTAAGCATCGTGGCGCT
  GGTTGGGGGGGTCCTCATCATCATCGCCTCCTGCGTCATCAACTTAGGCGTGTATAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214094 representing NM_001102563
  Red=Cloning site Green=Tags(s)
  
  MAASSSQVSEMKGVEDSSKTQTEGPRHSEEGLGPVQVVAEIPDQPEALQPGPGITAAPVDSGPKAELAPE
  TTETPVETPETVQATDLSLNPEEGSKASPSPSPSEARQEPASKPDVNRETAAEEGSEPQSTAPPEPTSEP
  AFQINTQSDPQPTSQPPPKPPLQAEPPTQEDPTTEVLTESTGEKQENGAVVPLQAGDGEEGPAPQPHSPP
  STKTPPANGAPPRVLQKLVEEDRIGRAHGGHPGSPRGSLSRHPSSQLAGPGVEGGEGTQKPRDYIILAIL
  SCFCPMWPVNIVAFAYAVMSRNSLQQGDVDGAQRLGRVAKLLSIVALVGGVLIIIASCVINLGVYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102563
• ORF Size: 1038 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102563.1, NP_001096033.1
• RefSeq Size: 2510 bp
• RefSeq ORF: 1041 bp
• Locus ID: 69017
• UniProt ID: E9PUL5
• Cytogenetics: 7 F3
• Gene Summary: As a component of the outer core of AMPAR complex, may be involved in synaptic transmission in the central nervous system. In hippocampal neurons, in presynaptic terminals, plays an important role in the final steps of neurotransmitter release, possibly by regulating Ca(2+)-sensing (PubMed:27052163). In the cerebellum, may inhibit SNARE complex formation and downregulate short-term facilitation (PubMed:29056747).[UniProtKB/Swiss-Prot Function]