Strip2 (NM_177204) Mouse Tagged ORF Clone

CAT#: MG213983

Strip2 (tGFP-tagged) - Mouse family with sequence similarity 40 member B (Fam40b) transcript variant 1, (10ug)

Product Images

Circular map for MG213983

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Strip2
• Synonyms: D330017J20Rik; Fam40b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG213983 representing NM_177204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCCCGCGGCACCGGGGCCCGCCGGCTCTCCTGCTAATGACAACGGCAACGGGAACGGCAATG
  GGAACGGGAACGGCAACGGCGGCAAAGGGAAGCCGGCCGTGCCCAAGGGCAGGGAAACGTTCCGCAACCA
  GCGGCGGGAGTCGGAGGGCTCTGTGGACTGCCCTACTCTGGAGTTTGAATATGGAGATTCAGACGGGCAT
  GCAGCAGAATTGTCAGAATTATATAGCTACACGGAGAACCTGGAATTTACCACTAACAGGAAGTGCTTTG
  AAGAAGATTTCAGGACTCAAGTGCAAGACACTAAGGAATGGCTGGAGTTGGAAGAAGATGCCCAGAAGAC
  CTATGTGATGGGACTTCTGGACCGACTGGAGGTGGTCAGTAGGGAGAAACGGCTTAAGGTGGCCCGGGCT
  GTTCTCTATCTAGCTCAAGGCACTTTCGGGGAATGTGATTCCGAGGTCGATGTGCTACACTGGTCCAGAT
  ACAATTGCTTCCTGCTCTACCAGATGGGGACCTTCTCGGCCTTCCTGGAGCTACTCCACATGGAAATCGA
  CAACAGCCAGGCCTCGAGTAGTGCCCTTCGGAAACCAGCTGTCTCCATTGCTGACAGCACTGAGCTCAGG
  GTACTGCTGAGTGTCATGTACCTAATGGTGGAAAATATCCGCCTGGAGCGAGAAATAGACCCTTGTGGGT
  GGAGGACAGCCCGAGAGACCTTCCGAACTGAACTGAGTTTCTCCACACATAATGAAGAGCCGTTTGCACT
  CCTGCTGTTTTCCATGGTTACCAAATTCTGCAGTGGCCTGGCCCCCCACTTTCCAATAAAGAAGGTTCTG
  CTTCTGCTCTGGAAGGTGGTCATGTTTACCCTTGGTGGATTTGAGCACCTTCAGGCTCTCAAAATACAGA
  AGCGTGCAGAGTTGGGCCTGCCTCCACTAGCTGAAGACAGTATCCAGGTTGTAAAGAGCATGCGTGCTGC
  CTCCCCGCCTTCTTACACTCTTGATCTGGGGGAGTCTCAGCTGGCTCCTCCACCCTCCAAGCTGCGGGGA
  CGCCGTGGTTCTCGAAGGCAACTCCTCACCAAGCAAGACAGCCTGGACATCTACAATGAAAGGGATCTCT
  TCAAGACTGAGGAACCAGCCACAGAGGAGGAAGAAGAATCTGCGGCTGATGGAGAACGAACCTTGGATGG
  AGAGTTAGACCTGCTAGAACAGGATCCTCTGGTGCCACCTCCACCCTCACAGACACCTCTCTCTACTGAC
  CGGGTAGCCTTTCCCAAGGGCCTGCCCTGGGCCCCGAAGGTTAGACAGAAGGATATTGAACACTTCTTGG
  AGATGAGCAGAAACAAGTTCATTGGATTCACTCTGGGGCAGGACACAGATACCTTGGTTGGATTACCTCG
  ACCCATCCATGAGAGTGTGAAGACTCTAAAGCAGCACAAGTATATCTCCATTGCAGATATACAGATAAAG
  AATGAAGAGGAGCTGGAGAAATGTCCCTTGTCTTTGGGAGAAGAGGTGGTACCAGAAACACCGAGTGAAA
  TCCTCTACCAGGGCATGCTGTACAGCCTCCCCCAGTACATGATTGCACTGCTTAAGATTCTGCTGGCTGC
  AGCTCCCACCTCCAAGGCTAAGACAGACTCTATCAATATCCTGGCAGATGTCCTGCCTGAAGAGATGCCT
  GTAACTGTTCTCCAGAGCATGAAGCTAGGCATTGACGTGAACAGACACAAGGAAATCATTGTAAAGAGTA
  TCTCTGCCCTGCTCCTTCTACTTCTCAAACACTTCAAACTCAACCATATCTACCAGTTTGAATATGTATC
  ACAACATTTAGTGTTTGCCAACTGCATCCCCTTGATCCTGAAGTTCTTCAATCAAAACATCCTGTCCTAT
  ATCACTGCCAAAAACAGCATTTCAGTGCTGGATTACCCGTGCTGCACCATCCAGGATTTGCCAGAGCTTA
  CTACCGAGAGTCTGGAAGCCGGAGACAATAGCCAGTTCTGCTGGAGGAACCTCTTTTCCTGCATCAACCT
  CCTGAGGCTGCTCAATAAGCTGACCAAATGGAAGCATTCCAGGACCATGATGTTAGTGGTGTTTAAGTCA
  GCTCCAATCTTAAAGCGAGCTCTCAAAGTCAAACAGGCCATGCTACAACTTTATGTCCTGAAGCTACTAA
  AGATACAGACCAAGTACCTTGGGCGCCAGTGGAGAAAAAGCAACATGAAGACCATGTCAGCCATTTACCA
  GAAAGTGCGCCACCGCATGAATGATGACTGGGCTTATGGGAATGACATCGATGCCAGGCCATGGGACTTC
  CAAGCAGAAGAATGTACCCTGAGGGCCAACATTGAGGCTTTTAACAGCCGCCGATATGATAAGCCTCAGG
  ACTCAGAATTCTCACCTGTGGATAACTGCTTGCAGAGCGTGCTGGGGCAGAGGCTAGATCTGCCTGAAGA
  TTTCCATTATTCCTATGAGCTCTGGCTGGAGAGAGAGGTGTTCTCACAGCCCATCTGCTGGGAGGAGCTG
  CTCCAGAATCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG213983 representing NM_177204
  Red=Cloning site Green=Tags(s)
  
  MDDPAAPGPAGSPANDNGNGNGNGNGNGNGGKGKPAVPKGRETFRNQRRESEGSVDCPTLEFEYGDSDGH
  AAELSELYSYTENLEFTTNRKCFEEDFRTQVQDTKEWLELEEDAQKTYVMGLLDRLEVVSREKRLKVARA
  VLYLAQGTFGECDSEVDVLHWSRYNCFLLYQMGTFSAFLELLHMEIDNSQASSSALRKPAVSIADSTELR
  VLLSVMYLMVENIRLEREIDPCGWRTARETFRTELSFSTHNEEPFALLLFSMVTKFCSGLAPHFPIKKVL
  LLLWKVVMFTLGGFEHLQALKIQKRAELGLPPLAEDSIQVVKSMRAASPPSYTLDLGESQLAPPPSKLRG
  RRGSRRQLLTKQDSLDIYNERDLFKTEEPATEEEEESAADGERTLDGELDLLEQDPLVPPPPSQTPLSTD
  RVAFPKGLPWAPKVRQKDIEHFLEMSRNKFIGFTLGQDTDTLVGLPRPIHESVKTLKQHKYISIADIQIK
  NEEELEKCPLSLGEEVVPETPSEILYQGMLYSLPQYMIALLKILLAAAPTSKAKTDSINILADVLPEEMP
  VTVLQSMKLGIDVNRHKEIIVKSISALLLLLLKHFKLNHIYQFEYVSQHLVFANCIPLILKFFNQNILSY
  ITAKNSISVLDYPCCTIQDLPELTTESLEAGDNSQFCWRNLFSCINLLRLLNKLTKWKHSRTMMLVVFKS
  APILKRALKVKQAMLQLYVLKLLKIQTKYLGRQWRKSNMKTMSAIYQKVRHRMNDDWAYGNDIDARPWDF
  QAEECTLRANIEAFNSRRYDKPQDSEFSPVDNCLQSVLGQRLDLPEDFHYSYELWLEREVFSQPICWEEL
  LQNH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177204
• ORF Size: 2532 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177204.3, NP_796178.2
• RefSeq Size: 5370 bp
• RefSeq ORF: 2535 bp
• Locus ID: 320609
• UniProt ID: Q8C9H6
• Cytogenetics: 6 A3.3
• Gene Summary: Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity).[UniProtKB/Swiss-Prot Function]