Prdm15 (NM_144789) Mouse Tagged ORF Clone

CAT#: MG213804

Prdm15 (tGFP-tagged) - Mouse PR domain containing 15 (Prdm15), (10ug)

Product Images

Circular map for MG213804

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prdm15
• Synonyms: C21orf83; E130018M06Rik; ORF62; Zfp298
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG213804 representing NM_144789
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCCACCTACTATCTGGGAAAAGGGTGGACAGGTGGGGGCCAGGTGGAGCCTGCGTGCTCCTGAGG
  TATCAGCAATGGCTGAAGATGGTAGTGAGGAGATCATGTTTATCTGGTGTGAAGACTGCAGCCAGTACCA
  TGACTCGGAGTGTCCTGAACTGGGACCTGTGGTCATGGTCAAGGACTCCTTTGTGCTGAGCAGGGCAAGG
  TCCTCCCTCCCCTCTAACCTGGAGATCAGGCGCCTGGATGACGGGGCTGAAGGCGTGTTTGCAGTGACCC
  AGCTAGTCAAGCGCACACAATTTGGTCCGTTTGAGTCCAGGAGAGTCGCCAAGTGGGAGAAAGAGTCGGC
  ATTTCCTCTGAAGGTATTTCAGAAGGACGGGCATCCTGTGTGCTTCGACACTTCCAATGAGGATGACTGC
  AACTGGATGATGCTAGTGCGACCAGCACTGGAGCCTGGGCACCAGAACCTGACAGCCTACCAACATGGCA
  GTGACGTGTATTTCACCACCTCAAAGGACATTCCTGCAGGCACTGAACTGCGTGTGTGGTATGCTGCCTT
  TTATGCCAAGAAGATGGACAAGCCCATGCTAAAGCAGGCCTGCTCCAGTGTTCAGGCTGCAGGCACCCCT
  GAGCCCAGTGTCTCTGTGGAACCTGAGCGTGGCCAATGGGTGTGTAAGGTGTGCTCCAACACCTTCCTGG
  AGCTTCAGCTCCTGAACGAGCATCTCTTGGGCCATCTGGAGCAAGCCAAAAGCCTCCCTGCAGGTGGCCA
  GCAGCATGAGGCAGCTTCTGAGAAGGAGCCTGATGCACCCAGGATGGAGCCTCCCACAGCAGCCGAGAGC
  AAGAGCATCCAGAGTGTCATGGTCACCAAAGAGCCAAAGAAAAAGCCTCGAAGAGGGAGGAAACCCAAAG
  CATCCAAGGTTGAGCAGCCTCTGGTCATTATCAAAGACAAGGAGCCTTCAGAGCATGTGGCAGAGATCAT
  AACTGAGATCCCCCCAGATGAGCCTGTGAGTGCAACACCAGATGAACGGATCATGGAGTTAGTCTTGGGG
  AAGCTGGCCGCCCCCACAAATGAAGCCAGCTCAGTGCCAAAGTTCCCACATCATCCAAGCAGCACCATTG
  CCCTCAAGAGGGGCTTGGTTCTGTCCAGCAGACATGGCGTCCGGCGGAAGCTCGTCCGTCAGCTGGGTGA
  GCATAAACGCATCCATCAGTGTGGCACCTGCAGCAAGGTCTTCCAGAACAGCAGCAACCTGAGCCGGCAC
  GTGCGCTCGCACGGTGAGTGCGCGCACGGGGACAAGCTGTTTAAATGTGAGGAATGTTCGAAACTGTTCA
  GCCGTAAGGAAAGTCTAAAGCAGCATGTTTCCTACAAGCACAGCCGGAATGAGGTGGACGGTGAGTACCG
  CTACCGCTGTGGCAGCTGTGGGAAGACATTCCGAATGGAGAGTGCACTGGAGTTCCACAACTGTAGGACA
  GATGACAAGACGTTCCAATGTGAGATGTGTTTCAGATTCTTCTCCACCAACAGCAACCTCTCTAAGCACA
  AGAAGAAACATGGGGACAAGAAGTTTGCCTGTGAGGTCTGCAGTAAGATGTTCTACCGCAAGGATGTTAT
  GCTGGACCACCAGAGGCGACACTTGGATGGTGTGCGGCGGGTGAAGAGAGAAGACTTGGAGGCCAGCGGG
  GAAAGCCTGGTTCGTTACAAGAAGGAACCTTCTGGATGCCCAGTGTGTGGCAAGGTGTTCTCCTGTCGGA
  GCAACATGAACAAGCACCTGCTGACCCACGGTGACAAGAAGTACACCTGCGAGATCTGTGGGCGCAAGTT
  CTTCCGAGTGGACGTACTCAGGGACCACATCCATGTCCACTTCAAGGACATCGCGCTAATGGATGACCAC
  CAGCGGGAGGAGTTCATTGGCAAAATTGGGATCTCCTCAGAGGAGAATGATGACAACTCTGATGAGAGCG
  CAGACTCTGAGCCACACAAGTACAGCTGCAAGAGGTGCCAGCTCACTTTCGGCCGTGGGAAGGAATACCT
  GAAGCACATCATGGAGGTGCACAAGGAGAAGGGCCACGGCTGCAGCATCTGCCACCGACGCTTCGCACTC
  AAGGCCACCTACCATGCCCACATGGTCATCCACCGGGAGAACCTGCCTGACCCCAACGTGCAGAAGTACA
  TTCACCCCTGTGAGATCTGTGGGAGGATATTCAACAGCATTGGGAACTTGGAGCGCCACAAACTCATCCA
  CACAGGTGTGAAAAGCCACGCCTGTGAGCAGTGTGGGAAGTCCTTTGCCAGGAAGGACATGCTGAAAGAG
  CACATGCGTGTGCATGACAACATCCGGGAGTACCTGTGCGCCGAGTGTGGGAAAGGTATGAAGACCAAGC
  ATGCACTGCGCCACCATATGAAGCTGCACAAAGGCATCAAGGAGTATGAGTGCAAGGAATGTCACCGCAA
  GTTTGCACAGAAGGTCAACATGCTCAAGCACTACAAACGGCACACAGGAATTAAAGACTTCATGTGTGAA
  TTGTGTGGGAAGACATTCAGTGAGAGGAACACAATGGAGACACACAAGCTCATCCACACAGTAGGCAAGC
  AGTGGACTTGTTCCGTGTGTGACAAGAAGTACGTCACAGAGTACATGCTTCAGAAACACGTCCAGCTCAC
  TCATGACAAGGTGGAAGCACAGAGTTGCCAACTATGTGGCACCAAAGTGTCAACCCGGGCCTCCATGAGC
  AGACATATGCGACGCAAGCACCCCGAGGTCCTCGCTGTTCGGATTGACGATCTGGACCACCTCCCCGAGA
  CCACCACCATCGATGCATCTTCCATCGGCATTGTCCAGCCTGCGCTGGGCCTGGAGCAGGAAGAACTGGC
  AGAAGGTAAGCATGGAAAGGCTGCCAAGCGCAGCCACAAGAGAAAGCAGAAGCCAGAGGAAGAGGCAGGG
  GCTCCAGTGCCTGAGGATACCACCTTCAGTGAATACCCCGAAAAGGAGCCAGAGTTCACAGGCAGTGTGG
  GTGACGAGACAAATTCTGCTGTACAGAGTATCCAACAGGTGGTGGTGACCCTAGGGGACCCCAATGTGAC
  TGCTCCATCAAGCTCAGTTGGTCTAACCAACATCACCGTGACCCCCATCACCACTGCAGCTGGAACTCAG
  TTTACCAATTTACAGCCAGTGGCTGTTGGACATCTTACCAACCCTGATCGCCAGCTACAGCTAGATAACT
  CCATCCTGACTGTGACCTTTGATACTGTCAGTGGCTCCGCCATGTTGCACAACCGGCAAAATGATGTCCA
  GATCCACCCACAGCCAGAAGCCACAAACCCTCAGTCAGTGGCCCACTTTATCAACCTTACCACACTGGTA
  AACTCCATCACACCCTTGGGGAACCAACTCAGTGAGCAGCACCCACTCACCTGGCGAGCAGTGCCCCAGA
  CAGATGTGCTGCAGCCGCCACAGGCCCCTGCAGCCCCGCAGCAGGCGGTGCAACCCCAGGTACAGAATGA
  GCAGCAGCAGATGTACAGCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG213804 representing NM_144789
  Red=Cloning site Green=Tags(s)
  
  MCPPTIWEKGGQVGARWSLRAPEVSAMAEDGSEEIMFIWCEDCSQYHDSECPELGPVVMVKDSFVLSRAR
  SSLPSNLEIRRLDDGAEGVFAVTQLVKRTQFGPFESRRVAKWEKESAFPLKVFQKDGHPVCFDTSNEDDC
  NWMMLVRPALEPGHQNLTAYQHGSDVYFTTSKDIPAGTELRVWYAAFYAKKMDKPMLKQACSSVQAAGTP
  EPSVSVEPERGQWVCKVCSNTFLELQLLNEHLLGHLEQAKSLPAGGQQHEAASEKEPDAPRMEPPTAAES
  KSIQSVMVTKEPKKKPRRGRKPKASKVEQPLVIIKDKEPSEHVAEIITEIPPDEPVSATPDERIMELVLG
  KLAAPTNEASSVPKFPHHPSSTIALKRGLVLSSRHGVRRKLVRQLGEHKRIHQCGTCSKVFQNSSNLSRH
  VRSHGECAHGDKLFKCEECSKLFSRKESLKQHVSYKHSRNEVDGEYRYRCGSCGKTFRMESALEFHNCRT
  DDKTFQCEMCFRFFSTNSNLSKHKKKHGDKKFACEVCSKMFYRKDVMLDHQRRHLDGVRRVKREDLEASG
  ESLVRYKKEPSGCPVCGKVFSCRSNMNKHLLTHGDKKYTCEICGRKFFRVDVLRDHIHVHFKDIALMDDH
  QREEFIGKIGISSEENDDNSDESADSEPHKYSCKRCQLTFGRGKEYLKHIMEVHKEKGHGCSICHRRFAL
  KATYHAHMVIHRENLPDPNVQKYIHPCEICGRIFNSIGNLERHKLIHTGVKSHACEQCGKSFARKDMLKE
  HMRVHDNIREYLCAECGKGMKTKHALRHHMKLHKGIKEYECKECHRKFAQKVNMLKHYKRHTGIKDFMCE
  LCGKTFSERNTMETHKLIHTVGKQWTCSVCDKKYVTEYMLQKHVQLTHDKVEAQSCQLCGTKVSTRASMS
  RHMRRKHPEVLAVRIDDLDHLPETTTIDASSIGIVQPALGLEQEELAEGKHGKAAKRSHKRKQKPEEEAG
  APVPEDTTFSEYPEKEPEFTGSVGDETNSAVQSIQQVVVTLGDPNVTAPSSSVGLTNITVTPITTAAGTQ
  FTNLQPVAVGHLTNPDRQLQLDNSILTVTFDTVSGSAMLHNRQNDVQIHPQPEATNPQSVAHFINLTTLV
  NSITPLGNQLSEQHPLTWRAVPQTDVLQPPQAPAAPQQAVQPQVQNEQQQMYSY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144789
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144789.2, NP_659038.2
• RefSeq Size: 6346 bp
• RefSeq ORF: 3525 bp
• Locus ID: 114604
• UniProt ID: E9Q8T2
• Cytogenetics: 16 C4
• Gene Summary: Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Plays also a role in induced pluripotent stem cells (iPSCs) reprogramming. Involved in early embryo development.[UniProtKB/Swiss-Prot Function]